GproteinDb

GproteinDb (G protein database) offers:

  • Reference data (see Data box)
  • Analysis tools (browse menu)
  • Visualization (e.g., snakeplots)
  • Experiment design (Coupling determinant mutations)
  • Data deposition (for G protein couplings and interface mutations)


G proteins are the main mediators of GPCR signaling1. Their activation is followed by recruitment of arrestins that arrest (stop) signaling or scaffold additional signaling proteins. There are 16 human G protein subtypes from 4 families that can be involved in biased signaling.

Data

  • Sequences
  • 16 Human G proteins
  • 317 Species orthologs
  • Couplings
  • 3,730 G protein couplings
  • Structures
  • 590 G proteins
  • 460 G protein complexes
  • 539 Generic residues
  • Structure models
  • 3,058 G protein complexes
  • 322 Refined complexes
  • Structure interactions
  • 13,281 G protein interface
  • Mutations
  • 54 Interface mutations

Latest release

March 9, 2023
  • 195 new structures added (up until December 2022)
  • New automated structure segment annotation based on DSSP and custom rules
  • New interaction calculation process using RDKit for higher precision and accuracy
  • Van der Waals interactions added
  • Class C activation state reverted to author based annotation instead of degree active calculation
  • New API calls for ligand data
  • Hydrogen atoms removed from AlphaFold models
  • GPCR - G protein interface interactions figures are now downloadable
  • Structure annotation fixes
  • Minor bug and performance fixes

All releases

Related resources

GPCRdb, GproteinDb, ArrestinDb and Biased Signaling Atlas cover related aspects of signal transduction while serving dedicated research communities and use cases. Together, they have served 7,257/52,724 users in the last month/year (Google Analytics)

Publications

The G protein database, GproteinDb.
Pandy-Szekeres G, Esguerra M, Hauser AS, Caroli J, Munk C, Pilger S, Keseru GM, Kooistra AJ, Gloriam DE
Nucleic acids research, 2022, 50:D518-D525