G protein coupling datasets deposited in GproteinDb

In addition to the subtype-level couplings, GproteinDb includes family-level couplings from the Guide to Pharmacology database (1). Biosensors are described in the Biosensors page.

Lab Biosensor Parameter Year Ref #Rec #wt
Gs Gi/o Gq/11 G12/13 Year
GsS GsL Golf Gi1 Gi2 Gi3 GoA GoB Gz Ggust Gq G11 G14 G15 G12 G13
Bouvier EMTA / GEMTA log(Emax/EC50) 2022 (2) 100 12 2022
Inoue NanoBiT-G log(Emax/EC50) 2019 (3) 8 8 2024
Inoue TGFα-shedding log(Emax/EC50) 2019 (3) 150 1* 2022 *Only Gq
is wt/used
Lambert RGB-GDP Efficacy - Unp 14 6 2024 Adhesions
Lambert RGB-GDP Econst., Efficacy - Unp 8 4 2024
Lambert RGB-GDP Econst., Efficacy 2019 (4) 16 4 2024
Lambert RGB-GDP Econst., Efficacy 2020 (5) 3 4 2024
Lambert RGB-GDP Econst. 2021 (6) 49 5 2024 Orphans
Martemyanov FReeβγ-Nluc Activation rate (s-1) 2023 (7) 117 5 2024
Roth TRUPATH log(Emax/EC50) 2020 (8) 4 15 2022


1. Harding, S.D., Armstrong, J.F., Faccenda, E., Southan, C., Alexander, S.P.H., Davenport, A.P., Pawson, A.J., Spedding, M., Davies, J.A. and Nc, I. (2022) The IUPHAR/BPS guide to PHARMACOLOGY in 2022: curating pharmacology for COVID-19, malaria and antibacterials. Nucleic Acids Res., 50, D1282-D1294.

2. Avet, C., Mancini, A., Breton, B., Le Gouill, C., Hauser, A.S., Normand, C., Kobayashi, H., Gross, F., Hogue, M., Lukasheva, V. et al. (2022) Effector membrane translocation biosensors reveal G protein and betaarrestin coupling profiles of 100 therapeutically relevant GPCRs. Elife, 11, 2020.2004.2020.052027.

3. Inoue, A., Raimondi, F., Kadji, F.M.N., Singh, G., Kishi, T., Uwamizu, A., Ono, Y., Shinjo, Y., Ishida, S., Arang, N. et al. (2019) Illuminating G-Protein-Coupling Selectivity of GPCRs. Cell, 177, 1933-1947 e1925.

4. Okashah, N., Wan, Q., Ghosh, S., Sandhu, M., Inoue, A., Vaidehi, N. and Lambert, N.A. (2019) Variable G protein determinants of GPCR coupling selectivity. Proceedings of the National Academy of Sciences, 116, 12054-12059.

5. Okashah, N., Wright, S.C., Kawakami, K., Mathiasen, S., Zhou, J., Lu, S., Javitch, J.A., Inoue, A., Bouvier, M. and Lambert, N.A. (2020) Agonist-induced formation of unproductive receptor-G12 complexes. Proceedings of the National Academy of Sciences of the United States of America, 117, 21723-21730.

6. Lu, S., Jang, W., Inoue, A. and Lambert, N.A. (2021) Constitutive G protein coupling profiles of understudied orphan GPCRs. PLoS One, 16, e0247743.

7. Masuho, I., Kise, R., Gainza, P., Von Moo, E., Li, X., Tany, R., Wakasugi-Masuho, H., Correia, B.E. and Martemyanov, K.A. (2023) Rules and mechanisms governing G protein coupling selectivity of GPCRs. Cell reports, 42, 113173.

8. Olsen, R.H.J., DiBerto, J.F., English, J.G., Glaudin, A.M., Krumm, B.E., Slocum, S.T., Che, T., Gavin, A.C., McCorvy, J.D., Roth, B.L. and Strachan, R.T. (2020) TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat. Chem. Biol., 16, 841-849.