par2_human
NAME
5NDZ
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
par2_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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R
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S
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P
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S
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A
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A
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W
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L
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L
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10 |
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G
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A
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A
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I
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L
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L
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A
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A
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S
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L
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20 |
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S
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C
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S
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G
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T
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I
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Q
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G
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T
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N
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30 |
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R
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S
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S
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K
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G
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R
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S
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L
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I
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G
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40 |
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K
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V
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D
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G
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T
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S
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H
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V
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T
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G
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50 |
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||
K
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G
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V
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T
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V
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E
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T
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V
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F
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S
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60 |
V
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D
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E
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F
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S
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A
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S
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V
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L
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T
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70 |
TM1 | |||||||||
G
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K
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L
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T
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T
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V
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F
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L
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P
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I
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80 |
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|||||||||
V
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Y
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T
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I
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V
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F
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V
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V
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G
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L
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90 |
P
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S
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N
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G
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M
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A
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L
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W
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V
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F
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100 |
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|||||||||
ICL1 | TM2 | ||||||||
L
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F
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R
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T
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K
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K
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K
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H
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P
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A
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110 |
V
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I
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Y
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M
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A
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N
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L
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A
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L
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A
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120 |
D
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L
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L
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S
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V
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I
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W
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F
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P
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L
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130 |
K
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I
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A
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Y
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H
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I
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H
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G
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N
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N
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140 |
ECL1 | TM3 | ||||||||
W
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I
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Y
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G
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E
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A
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L
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C
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N
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V
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150 |
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|||||||||
L
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I
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G
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F
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F
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Y
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G
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N
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M
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Y
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160 |
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|||||||||
C
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S
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I
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L
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F
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M
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T
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C
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L
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S
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170 |
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||||||||
V
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Q
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R
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Y
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W
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V
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I
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V
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N
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P
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180 |
ICL2 | TM4 | ||||||||
M
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G
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H
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S
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R
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K
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K
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A
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N
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I
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190 |
A
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I
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G
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I
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S
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L
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A
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I
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W
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L
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200 |
L
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I
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L
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L
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V
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T
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I
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P
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L
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Y
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210 |
ECL2 | |||||||||
V
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V
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K
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Q
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T
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I
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F
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I
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P
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A
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220 |
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|||||||||
L
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N
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I
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T
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T
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C
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H
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D
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V
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L
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230 |
TM5 | |||||||||
P
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E
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Q
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L
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L
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V
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G
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D
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M
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F
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240 |
N
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Y
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F
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L
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S
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L
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A
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I
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G
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V
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250 |
F
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L
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F
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P
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A
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F
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L
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T
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A
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S
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260 |
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||||||||
A
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Y
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V
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L
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M
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I
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R
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M
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L
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R
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270 |
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ICL3 | TM6 | ||||||||
S
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S
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A
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M
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D
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E
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N
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S
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E
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K
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280 |
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|||||||||
K
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R
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K
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R
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A
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I
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K
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L
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I
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V
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290 |
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|||||||||
T
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V
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L
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A
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M
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Y
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L
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I
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C
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F
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300 |
T
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P
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S
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N
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L
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L
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L
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V
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V
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H
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310 |
Y
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F
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L
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I
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K
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S
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Q
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G
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Q
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S
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320 |
ECL3 | TM7 | ||||||||
H
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V
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Y
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A
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L
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Y
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I
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V
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A
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L
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330 |
C
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L
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S
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T
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L
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N
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S
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C
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I
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D
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340 |
H8 | |||||||||
P
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F
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V
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Y
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Y
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F
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V
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S
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H
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D
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350 |
F
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R
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D
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H
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A
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K
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N
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A
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L
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L
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360 |
C-term | |||||||||
C
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R
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S
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V
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R
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T
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V
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K
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Q
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M
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370 |
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Q
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V
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S
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L
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T
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S
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K
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K
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H
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S
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380 |
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R
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K
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S
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S
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S
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Y
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S
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S
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S
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S
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390 |
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T
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T
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V
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K
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T
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S
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Y
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GP64 envelo | ||||||||||
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T4 Lysozyme | ||||||||||
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N-term | |||||||||
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F
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S
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V
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D
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E
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F
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S
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A
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S
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67 |
TM1 | |||||||||
V
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L
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T
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G
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K
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L
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T
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T
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V
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F
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77 |
L
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P
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I
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V
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Y
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T
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I
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V
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F
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V
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87 |
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|||||||||
V
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A
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L
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P
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S
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N
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G
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M
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A
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L
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97 |
ICL1 | |||||||||
W
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V
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F
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L
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F
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R
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T
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K
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K
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K
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107 |
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TM2 | |||||||||
A
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P
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A
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V
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I
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Y
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M
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A
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N
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L
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117 |
A
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L
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A
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D
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L
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L
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S
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V
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I
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W
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127 |
F
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P
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L
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K
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I
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A
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Y
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H
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I
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H
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137 |
ECL1 | TM3 | ||||||||
G
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N
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N
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W
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I
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Y
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G
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E
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A
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L
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147 |
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|||||||||
C
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N
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V
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L
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I
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G
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F
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F
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Y
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A
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157 |
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|||||||||
N
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M
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Y
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C
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S
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I
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L
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F
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L
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T
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167 |
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||||||||
C
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L
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S
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V
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Q
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R
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A
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W
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E
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I
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177 |
ICL2 | |||||||||
V
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N
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P
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M
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G
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H
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S
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R
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K
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K
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187 |
TM4 | |||||||||
A
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N
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I
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A
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I
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G
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I
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S
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L
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A
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197 |
I
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W
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L
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L
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I
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L
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L
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V
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T
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I
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207 |
ECL2 | |||||||||
P
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L
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Y
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V
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V
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K
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Q
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T
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I
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F
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217 |
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|||||||||
I
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P
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A
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L
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Q
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I
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T
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T
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C
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H
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227 |
TM5 | |||||||||
D
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V
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L
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P
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E
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Q
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L
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L
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V
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G
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237 |
D
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M
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F
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N
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Y
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F
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L
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S
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L
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A
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247 |
I
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G
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V
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F
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L
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F
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P
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A
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F
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L
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257 |
T
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A
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S
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A
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Y
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V
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L
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M
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I
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R
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267 |
|
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A
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L
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BRIL (b562R | ||||||||||
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|||||||
TM6 | |||||||
E
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N
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S
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E
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K
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K
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R
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K
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283 |
|
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||||||||
R
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A
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I
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K
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L
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A
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V
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T
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V
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A
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293 |
A
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M
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Y
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L
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I
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C
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F
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T
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P
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S
|
303 |
N
|
L
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L
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L
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V
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V
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H
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Y
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F
|
L
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313 |
ECL3 | |||||||||
I
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K
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S
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Q
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G
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Q
|
S
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H
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V
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Y
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323 |
TM7 | |||||||||
A
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L
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Y
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I
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V
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A
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L
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C
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L
|
S
|
333 |
T
|
L
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N
|
S
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C
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I
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D
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P
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F
|
V
|
343 |
H8 | |||||||||
Y
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Y
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F
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V
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S
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H
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D
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F
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R
|
D
|
353 |
H
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A
|
K
|
N
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A
|
L
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L
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C
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R
|
S
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363 |
C-term | |||||||||
V
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R
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T
|
V
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K
|
Q
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M
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Q
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V
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S
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373 |
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L
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T
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S
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K
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His(10) | |||||||||
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SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
89 | | G | A |
108 | | H | A |
157 | | G | A |
166 | | M | L |
174 | | Y | A |
176 | | V | E |
222 | | N | Q |
268 | | M | A |
289 | | I | A |
293 | | L | A |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: signal
Name: GP64 envelope protein
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: fusion
Name: T4 Lysozyme (T4L)
Sequence: None
Deletions:
Positions: N-term_1 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: fusion
Name: BRIL (b562RIL)
Sequence: None
Deletions:
Positions: Within Receptor_2 270:276
Presence in Crystal:YES
Remarks: None
Protein_type: tag
Name: His(10)
Sequence: None
Deletions:
Positions: C-term_3 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Sf9 | Insect |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | LMNG | 1.0 %w/v |
additive | CHS | 0.1 %w/v |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Imidazole to improve purity of histidine-tagged proteins | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 40 mg/ml
Temperature: 20°C
ph : 5.5-6.2
Remarks:
Chemical lists
Type | Item | Concentration |
---|---|---|
Detergent | ||
detergent | LMNG | 0.004 % w/v |
Lipid | ||
lipid | CHS | 0.002 %w/v |
LCP lipid additive | ||
cholesterol | 10.0 % | |
Buffer | ||
sodium citrate/citrate acid pH 5.5–6.2 | 0.1 M | |
Additional | ||
ammonium phosphate dibasic | 0.2 M | |
PEG 400 | 38–43 %(v/v) |