calrl_human
NAME
6UUN
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
calrl_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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E
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K
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K
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C
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T
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L
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Y
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F
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L
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10 |
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V
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L
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L
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P
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F
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F
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M
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I
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L
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V
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20 |
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|||||||||
T
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A
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E
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L
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E
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E
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S
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P
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E
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D
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30 |
S
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I
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Q
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L
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G
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V
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T
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R
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N
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K
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40 |
I
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M
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T
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A
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Q
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Y
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E
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C
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Y
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Q
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50 |
K
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I
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M
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Q
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D
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P
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I
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Q
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Q
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A
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60 |
E
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G
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V
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Y
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C
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N
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R
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T
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W
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D
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70 |
G
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W
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L
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C
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W
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N
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D
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V
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A
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A
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80 |
G
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T
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E
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S
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M
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Q
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L
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C
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P
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D
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90 |
Y
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F
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Q
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D
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F
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D
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P
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S
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E
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K
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100 |
V
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T
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K
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I
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C
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D
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Q
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D
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G
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N
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110 |
W
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F
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R
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H
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P
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A
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S
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N
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R
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T
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120 |
W
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T
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N
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Y
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T
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Q
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C
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N
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V
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N
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130 |
TM1 | |||||||||
T
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H
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E
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K
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V
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K
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T
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A
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L
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N
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140 |
L
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F
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Y
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L
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T
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I
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I
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G
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H
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G
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150 |
L
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S
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I
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A
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S
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L
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L
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I
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S
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L
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160 |
ICL1 | |||||||||
G
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I
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F
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F
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Y
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F
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K
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S
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L
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S
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170 |
TM2 | |||||||||
C
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Q
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R
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I
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T
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L
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H
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K
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N
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L
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180 |
F
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F
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S
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F
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V
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C
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N
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S
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V
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V
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190 |
T
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I
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I
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H
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L
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T
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A
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V
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A
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N
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200 |
ECL1 | |||||||||
N
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Q
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A
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L
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V
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A
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T
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N
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P
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V
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210 |
TM3 | |||||||||
S
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C
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K
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V
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S
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Q
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F
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I
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H
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L
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220 |
Y
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L
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M
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G
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C
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N
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Y
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F
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W
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M
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230 |
L
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C
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E
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G
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I
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Y
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L
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H
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T
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L
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240 |
ICL2 | |||||||||
I
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V
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V
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A
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V
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F
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A
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E
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K
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Q
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250 |
TM4 | |||||||||
H
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L
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M
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W
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Y
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Y
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F
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L
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G
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W
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260 |
G
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F
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P
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L
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I
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P
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A
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C
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I
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H
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270 |
A
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I
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A
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R
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S
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L
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Y
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Y
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N
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D
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280 |
ECL2 | TM5 | ||||||||
N
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C
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W
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I
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S
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S
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D
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T
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H
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L
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290 |
L
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Y
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I
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I
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H
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G
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P
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I
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C
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A
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300 |
A
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L
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L
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V
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N
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L
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F
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F
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L
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L
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310 |
N
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I
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V
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R
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V
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L
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I
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T
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K
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L
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320 |
ICL3 | |||||||||
K
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V
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T
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H
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Q
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A
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E
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S
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N
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L
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330 |
TM6 | |||||||||
Y
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M
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K
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A
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V
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R
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A
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T
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L
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I
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340 |
L
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V
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P
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L
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L
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G
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I
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E
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F
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V
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350 |
ECL3 | |||||||||
L
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I
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P
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W
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R
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P
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E
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G
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K
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I
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360 |
TM7 | |||||||||
A
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E
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E
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V
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Y
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D
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Y
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I
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M
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H
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370 |
I
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L
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M
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H
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F
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Q
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G
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L
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L
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V
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380 |
H8 | |||||||||
S
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T
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I
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F
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C
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F
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F
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N
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G
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E
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390 |
V
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Q
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A
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I
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L
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R
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R
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N
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W
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N
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400 |
Q
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Y
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K
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I
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Q
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F
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G
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N
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S
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F
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410 |
C-term | |||||||||
S
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N
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S
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E
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A
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L
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R
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S
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A
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S
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420 |
Y
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T
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V
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S
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T
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I
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S
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D
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G
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P
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430 |
G
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Y
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S
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H
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D
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C
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P
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S
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E
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H
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440 |
L
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N
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G
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K
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S
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I
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H
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D
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I
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E
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450 |
N
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V
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L
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L
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K
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P
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E
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N
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L
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Y
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460 |
N
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Initiating | ||||||||||
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N-term | |||||||||
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A
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E
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L
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E
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E
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S
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P
|
E
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D
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30 |
S
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I
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Q
|
L
|
G
|
V
|
T
|
R
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N
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K
|
40 |
I
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M
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T
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A
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Q
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Y
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E
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C
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Y
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Q
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50 |
K
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I
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M
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Q
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D
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P
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I
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Q
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Q
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A
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60 |
E
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G
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V
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Y
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C
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N
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R
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T
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W
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D
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70 |
G
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W
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L
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C
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W
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N
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D
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V
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A
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A
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80 |
G
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T
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E
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S
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M
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Q
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L
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C
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P
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D
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90 |
Y
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F
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Q
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D
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F
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D
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P
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S
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E
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K
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100 |
V
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T
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K
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I
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C
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D
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Q
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D
|
G
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N
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110 |
W
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F
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R
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H
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P
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A
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S
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N
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R
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T
|
120 |
W
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T
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N
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Y
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T
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Q
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C
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N
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V
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N
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130 |
TM1 | |||||||||
T
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H
|
E
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K
|
V
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K
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T
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A
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L
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N
|
140 |
L
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F
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Y
|
L
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T
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I
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I
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G
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H
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G
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150 |
L
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S
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I
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A
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S
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L
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L
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I
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S
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L
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160 |
ICL1 | |||||||||
G
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I
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F
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F
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Y
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F
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K
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S
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L
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S
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170 |
TM2 | |||||||||
C
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Q
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R
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I
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T
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L
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H
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K
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N
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L
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180 |
F
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F
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S
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F
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V
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C
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N
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S
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V
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V
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190 |
T
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I
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I
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H
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L
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T
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A
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V
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A
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N
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200 |
ECL1 | |||||||||
N
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Q
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A
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L
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V
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A
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T
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N
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P
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V
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210 |
TM3 | |||||||||
S
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C
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K
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V
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S
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Q
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F
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I
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H
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L
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220 |
Y
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L
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M
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G
|
C
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N
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Y
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F
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W
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M
|
230 |
L
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C
|
E
|
G
|
I
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Y
|
L
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H
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T
|
L
|
240 |
ICL2 | |||||||||
I
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V
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V
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A
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V
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F
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A
|
E
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K
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Q
|
250 |
TM4 | |||||||||
H
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L
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M
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W
|
Y
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Y
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F
|
L
|
G
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W
|
260 |
G
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F
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P
|
L
|
I
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P
|
A
|
C
|
I
|
H
|
270 |
A
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I
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A
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R
|
S
|
L
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Y
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Y
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N
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D
|
280 |
ECL2 | TM5 | ||||||||
N
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C
|
W
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I
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S
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S
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D
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T
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H
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L
|
290 |
L
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Y
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I
|
I
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H
|
G
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P
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I
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C
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A
|
300 |
A
|
L
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L
|
V
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N
|
L
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F
|
F
|
L
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L
|
310 |
N
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I
|
V
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R
|
V
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L
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I
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T
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K
|
L
|
320 |
ICL3 | |||||||||
K
|
V
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T
|
H
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Q
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A
|
E
|
S
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N
|
L
|
330 |
TM6 | |||||||||
Y
|
M
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K
|
A
|
V
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R
|
A
|
T
|
L
|
I
|
340 |
L
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V
|
P
|
L
|
L
|
G
|
I
|
E
|
F
|
V
|
350 |
ECL3 | |||||||||
L
|
I
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P
|
W
|
R
|
P
|
E
|
G
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K
|
I
|
360 |
TM7 | |||||||||
A
|
E
|
E
|
V
|
Y
|
D
|
Y
|
I
|
M
|
H
|
370 |
I
|
L
|
M
|
H
|
F
|
Q
|
G
|
L
|
L
|
V
|
380 |
H8 | |||||||||
S
|
T
|
I
|
F
|
C
|
F
|
F
|
N
|
G
|
E
|
390 |
V
|
Q
|
A
|
I
|
L
|
R
|
R
|
N
|
W
|
N
|
400 |
Q
|
Y
|
K
|
I
|
Q
|
F
|
G
|
N
|
S
|
F
|
410 |
C-term | |||||||||
S
|
N
|
S
|
E
|
A
|
L
|
R
|
S
|
A
|
S
|
420 |
Y
|
T
|
V
|
S
|
T
|
I
|
S
|
D
|
G
|
P
|
430 |
G
|
Y
|
S
|
H
|
D
|
C
|
P
|
S
|
E
|
H
|
440 |
L
|
N
|
G
|
K
|
S
|
I
|
H
|
D
|
I
|
E
|
450 |
N
|
V
|
L
|
L
|
K
|
P
|
E
|
N
|
L
|
Y
|
460 |
N
|
461 |
|
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|
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Expression | ||||||||||
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SCHEMATIC
Perspective:
Wild-type
Construct
|
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|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: auto
Name: Expression tag
Sequence: None
Deletions:
Positions: C-term_1 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: auto
Name: Initiating methionine
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|