5ht1d_human
NAME
7E32
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
5ht1d_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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S
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P
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L
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N
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Q
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S
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A
|
E
|
G
|
10 |
L
|
P
|
Q
|
E
|
A
|
S
|
N
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R
|
S
|
L
|
20 |
N
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A
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T
|
E
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T
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S
|
E
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A
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W
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D
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30 |
TM1 | |||||||||
P
|
R
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T
|
L
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Q
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A
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L
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K
|
I
|
S
|
40 |
L
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A
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V
|
V
|
L
|
S
|
V
|
I
|
T
|
L
|
50 |
A
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T
|
V
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L
|
S
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N
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A
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F
|
V
|
L
|
60 |
ICL1 | |||||||||
T
|
T
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I
|
L
|
L
|
T
|
R
|
K
|
L
|
H
|
70 |
TM2 | |||||||||
T
|
P
|
A
|
N
|
Y
|
L
|
I
|
G
|
S
|
L
|
80 |
A
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T
|
T
|
D
|
L
|
L
|
V
|
S
|
I
|
L
|
90 |
V
|
M
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P
|
I
|
S
|
I
|
A
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Y
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T
|
I
|
100 |
ECL1 | TM3 | ||||||||
T
|
H
|
T
|
W
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N
|
F
|
G
|
Q
|
I
|
L
|
110 |
C
|
D
|
I
|
W
|
L
|
S
|
S
|
D
|
I
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T
|
120 |
C
|
C
|
T
|
A
|
S
|
I
|
L
|
H
|
L
|
C
|
130 |
V
|
I
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A
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L
|
D
|
R
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Y
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W
|
A
|
I
|
140 |
ICL2 | |||||||||
T
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D
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A
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L
|
E
|
Y
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S
|
K
|
R
|
R
|
150 |
TM4 | |||||||||
T
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A
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G
|
H
|
A
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A
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T
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M
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I
|
A
|
160 |
I
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V
|
W
|
A
|
I
|
S
|
I
|
C
|
I
|
S
|
170 |
I
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P
|
P
|
L
|
F
|
W
|
R
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Q
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A
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K
|
180 |
ECL2 | |||||||||
A
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Q
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E
|
E
|
M
|
S
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D
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C
|
L
|
V
|
190 |
TM5 | |||||||||
N
|
T
|
S
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Q
|
I
|
S
|
Y
|
T
|
I
|
Y
|
200 |
S
|
T
|
C
|
G
|
A
|
F
|
Y
|
I
|
P
|
S
|
210 |
V
|
L
|
L
|
I
|
I
|
L
|
Y
|
G
|
R
|
I
|
220 |
Y
|
R
|
A
|
A
|
R
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N
|
R
|
I
|
L
|
N
|
230 |
ICL3 | |||||||||
P
|
P
|
S
|
L
|
Y
|
G
|
K
|
R
|
F
|
T
|
240 |
T
|
A
|
H
|
L
|
I
|
T
|
G
|
S
|
A
|
G
|
250 |
S
|
S
|
L
|
C
|
S
|
L
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N
|
S
|
S
|
L
|
260 |
H
|
E
|
G
|
H
|
S
|
H
|
S
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A
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G
|
S
|
270 |
P
|
L
|
F
|
F
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N
|
H
|
V
|
K
|
I
|
K
|
280 |
L
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A
|
D
|
S
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A
|
L
|
E
|
R
|
K
|
R
|
290 |
TM6 | |||||||||
I
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S
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A
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A
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R
|
E
|
R
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K
|
A
|
T
|
300 |
K
|
I
|
L
|
G
|
I
|
I
|
L
|
G
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A
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F
|
310 |
I
|
I
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C
|
W
|
L
|
P
|
F
|
F
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V
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V
|
320 |
S
|
L
|
V
|
L
|
P
|
I
|
C
|
R
|
D
|
S
|
330 |
ECL3 | TM7 | ||||||||
C
|
W
|
I
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H
|
P
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A
|
L
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F
|
D
|
F
|
340 |
F
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T
|
W
|
L
|
G
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Y
|
L
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N
|
S
|
L
|
350 |
I
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N
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P
|
I
|
I
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Y
|
T
|
V
|
F
|
N
|
360 |
H8 | |||||||||
E
|
E
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F
|
R
|
Q
|
A
|
F
|
Q
|
K
|
I
|
370 |
C-term | ||||||
V
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P
|
F
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R
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K
|
A
|
S
|
|
|||||||||
N-term | |||||||||
|
S
|
P
|
L
|
N
|
Q
|
S
|
A
|
E
|
G
|
10 |
L
|
P
|
Q
|
E
|
A
|
S
|
N
|
R
|
S
|
L
|
20 |
N
|
A
|
T
|
E
|
T
|
S
|
E
|
A
|
W
|
D
|
30 |
TM1 | |||||||||
P
|
R
|
T
|
L
|
Q
|
A
|
L
|
K
|
I
|
S
|
40 |
L
|
A
|
V
|
V
|
L
|
S
|
V
|
I
|
T
|
L
|
50 |
A
|
T
|
V
|
L
|
S
|
N
|
A
|
F
|
V
|
L
|
60 |
ICL1 | |||||||||
T
|
T
|
I
|
L
|
L
|
T
|
R
|
K
|
L
|
H
|
70 |
TM2 | |||||||||
T
|
P
|
A
|
N
|
Y
|
L
|
I
|
G
|
S
|
L
|
80 |
A
|
T
|
T
|
D
|
L
|
L
|
V
|
S
|
I
|
L
|
90 |
V
|
M
|
P
|
I
|
S
|
I
|
A
|
Y
|
T
|
I
|
100 |
ECL1 | TM3 | ||||||||
T
|
H
|
T
|
W
|
N
|
F
|
G
|
Q
|
I
|
L
|
110 |
C
|
D
|
I
|
W
|
L
|
S
|
S
|
D
|
I
|
T
|
120 |
C
|
C
|
T
|
A
|
S
|
I
|
L
|
H
|
L
|
C
|
130 |
V
|
I
|
A
|
L
|
D
|
R
|
Y
|
W
|
A
|
I
|
140 |
ICL2 | |||||||||
T
|
D
|
A
|
L
|
E
|
Y
|
S
|
K
|
R
|
R
|
150 |
TM4 | |||||||||
T
|
A
|
G
|
H
|
A
|
A
|
T
|
M
|
I
|
A
|
160 |
I
|
V
|
W
|
A
|
I
|
S
|
I
|
C
|
I
|
S
|
170 |
I
|
P
|
P
|
L
|
F
|
W
|
R
|
Q
|
A
|
K
|
180 |
ECL2 | |||||||||
A
|
Q
|
E
|
E
|
M
|
S
|
D
|
C
|
L
|
V
|
190 |
TM5 | |||||||||
N
|
T
|
S
|
Q
|
I
|
S
|
Y
|
T
|
I
|
Y
|
200 |
S
|
T
|
C
|
G
|
A
|
F
|
Y
|
I
|
P
|
S
|
210 |
V
|
L
|
L
|
I
|
I
|
L
|
Y
|
G
|
R
|
I
|
220 |
Y
|
R
|
A
|
A
|
R
|
N
|
R
|
I
|
L
|
N
|
230 |
ICL3 | |||||||||
P
|
P
|
S
|
L
|
Y
|
G
|
K
|
R
|
F
|
T
|
240 |
T
|
A
|
H
|
L
|
I
|
T
|
G
|
S
|
A
|
G
|
250 |
S
|
S
|
L
|
C
|
S
|
L
|
N
|
S
|
S
|
L
|
260 |
H
|
E
|
G
|
H
|
S
|
H
|
S
|
A
|
G
|
S
|
270 |
P
|
L
|
F
|
F
|
N
|
H
|
V
|
K
|
I
|
K
|
280 |
L
|
A
|
D
|
S
|
A
|
L
|
E
|
R
|
K
|
R
|
290 |
TM6 | |||||||||
I
|
S
|
A
|
A
|
R
|
E
|
R
|
K
|
A
|
T
|
300 |
K
|
I
|
L
|
G
|
I
|
I
|
L
|
G
|
A
|
F
|
310 |
I
|
I
|
C
|
W
|
L
|
P
|
F
|
F
|
V
|
V
|
320 |
S
|
L
|
V
|
L
|
P
|
I
|
C
|
R
|
D
|
S
|
330 |
ECL3 | TM7 | ||||||||
C
|
W
|
I
|
H
|
P
|
A
|
L
|
F
|
D
|
F
|
340 |
F
|
T
|
W
|
L
|
G
|
Y
|
L
|
N
|
S
|
L
|
350 |
I
|
N
|
P
|
I
|
I
|
Y
|
T
|
V
|
F
|
N
|
360 |
H8 | |||||||||
E
|
E
|
F
|
R
|
Q
|
A
|
F
|
Q
|
K
|
I
|
370 |
C-term | ||||||
V
|
P
|
F
|
R
|
K
|
A
|
S
|
377 |
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|