5ht2a_human
NAME
7RAN
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
5ht2a_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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D
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I
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L
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C
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E
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E
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N
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T
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S
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10 |
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|
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L
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S
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S
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T
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T
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N
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S
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L
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M
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Q
|
20 |
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|
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L
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N
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D
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D
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T
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R
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L
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Y
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S
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N
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30 |
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|
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|
|
|
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D
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F
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N
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S
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G
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E
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A
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N
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T
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S
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40 |
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|
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D
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A
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F
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N
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W
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T
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V
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D
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S
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E
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50 |
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N
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R
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T
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N
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L
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S
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C
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E
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G
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C
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60 |
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|||||
L
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S
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P
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S
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C
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L
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S
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L
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L
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H
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70 |
TM1 | |||||||||
L
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Q
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E
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K
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N
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W
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S
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A
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L
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L
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80 |
T
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A
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V
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V
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I
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I
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L
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T
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I
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A
|
90 |
G
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N
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I
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L
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V
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I
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M
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A
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V
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S
|
100 |
ICL1 | TM2 | ||||||||
L
|
E
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K
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K
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L
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Q
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N
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A
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T
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N
|
110 |
Y
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F
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L
|
M
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S
|
L
|
A
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I
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A
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D
|
120 |
M
|
L
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L
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G
|
F
|
L
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V
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M
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P
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V
|
130 |
S
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M
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L
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T
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I
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L
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Y
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G
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Y
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R
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140 |
ECL1 | TM3 | ||||||||
W
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P
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L
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P
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S
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K
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L
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C
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A
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V
|
150 |
W
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I
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Y
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L
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D
|
V
|
L
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F
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S
|
T
|
160 |
A
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S
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I
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M
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H
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L
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C
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A
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I
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S
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170 |
L
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D
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R
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Y
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V
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A
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I
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Q
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N
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P
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180 |
ICL2 | TM4 | ||||||||
I
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H
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H
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S
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R
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F
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N
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S
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R
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T
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190 |
K
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A
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F
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L
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K
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I
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I
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A
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V
|
W
|
200 |
T
|
I
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S
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V
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G
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I
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S
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M
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P
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I
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210 |
P
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V
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F
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G
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L
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Q
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D
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D
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S
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K
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220 |
ECL2 | |||||||||
V
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F
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K
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E
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G
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S
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C
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L
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L
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A
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230 |
TM5 | |||||||||
D
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D
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N
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F
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V
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L
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I
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G
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S
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F
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240 |
V
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S
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F
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F
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I
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P
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L
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T
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I
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M
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250 |
V
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I
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T
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Y
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F
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L
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T
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I
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K
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S
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260 |
L
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Q
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K
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E
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A
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T
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L
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C
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V
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S
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270 |
ICL3 | |||||||||
D
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L
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G
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T
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R
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A
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K
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L
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A
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S
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280 |
F
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S
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F
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L
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P
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Q
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S
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S
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L
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S
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290 |
S
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E
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K
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L
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F
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Q
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R
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S
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I
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H
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300 |
R
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E
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P
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G
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S
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Y
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T
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G
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R
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R
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310 |
TM6 | |||||||||
T
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M
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Q
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S
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I
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S
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N
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E
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Q
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K
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320 |
A
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C
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K
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V
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L
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G
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I
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V
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F
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F
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330 |
L
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F
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V
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V
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M
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W
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C
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P
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F
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F
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340 |
I
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T
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N
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I
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M
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A
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V
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I
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C
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K
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350 |
ECL3 | TM7 | ||||||||
E
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S
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C
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N
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E
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D
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V
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I
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G
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A
|
360 |
L
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L
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N
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V
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F
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V
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W
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I
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G
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Y
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370 |
L
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S
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S
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A
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V
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N
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P
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L
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V
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Y
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380 |
H8 | |||||||||
T
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L
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F
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N
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K
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T
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Y
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R
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S
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A
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390 |
F
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S
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R
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Y
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I
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Q
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C
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Q
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Y
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K
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400 |
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||||
C-term | |||||||||
E
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N
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K
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K
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P
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L
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Q
|
L
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I
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L
|
410 |
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|
|
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V
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N
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T
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I
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P
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A
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L
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A
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Y
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K
|
420 |
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|
|
|
|
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S
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S
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Q
|
L
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Q
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M
|
G
|
Q
|
K
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K
|
430 |
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|
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|
|
|
|
|
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N
|
S
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K
|
Q
|
D
|
A
|
K
|
T
|
T
|
D
|
440 |
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|
|
|
|
|
|
|
|
|
N
|
D
|
C
|
S
|
M
|
V
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A
|
L
|
G
|
K
|
450 |
|
|
|
|
|
|
|
|
|
|
Q
|
H
|
S
|
E
|
E
|
A
|
S
|
K
|
D
|
N
|
460 |
|
|
|
|
|
|
|
|
|
|
S
|
D
|
G
|
V
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N
|
E
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K
|
V
|
S
|
C
|
470 |
|
V
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|||||||||
N-term | TM1 | ||||||||
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L
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S
|
L
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L
|
H
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L
|
Q
|
E
|
K
|
74 |
N
|
W
|
S
|
A
|
L
|
L
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T
|
A
|
V
|
V
|
84 |
I
|
I
|
L
|
T
|
I
|
A
|
G
|
N
|
I
|
L
|
94 |
V
|
I
|
M
|
A
|
V
|
S
|
L
|
E
|
K
|
K
|
104 |
ICL1 | TM2 | ||||||||
L
|
Q
|
N
|
A
|
T
|
N
|
Y
|
F
|
L
|
M
|
114 |
S
|
L
|
A
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I
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A
|
D
|
M
|
L
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L
|
G
|
124 |
F
|
L
|
V
|
M
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P
|
V
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S
|
M
|
L
|
T
|
134 |
ECL1 | |||||||||
I
|
L
|
Y
|
G
|
Y
|
R
|
W
|
P
|
L
|
P
|
144 |
TM3 | |||||||||
S
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K
|
L
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C
|
A
|
V
|
W
|
I
|
Y
|
L
|
154 |
D
|
V
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L
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F
|
S
|
T
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A
|
S
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I
|
M
|
164 |
H
|
L
|
C
|
A
|
I
|
S
|
L
|
D
|
R
|
Y
|
174 |
ICL2 | |||||||||
V
|
A
|
I
|
Q
|
N
|
P
|
I
|
H
|
H
|
S
|
184 |
TM4 | |||||||||
R
|
F
|
N
|
S
|
R
|
T
|
K
|
A
|
F
|
L
|
194 |
K
|
I
|
I
|
A
|
V
|
W
|
T
|
I
|
S
|
V
|
204 |
G
|
I
|
S
|
M
|
P
|
I
|
P
|
V
|
F
|
G
|
214 |
ECL2 | |||||||||
L
|
Q
|
D
|
D
|
S
|
K
|
V
|
F
|
K
|
E
|
224 |
TM5 | |||||||||
G
|
S
|
C
|
L
|
L
|
A
|
D
|
D
|
N
|
F
|
234 |
V
|
L
|
I
|
G
|
S
|
F
|
V
|
S
|
F
|
F
|
244 |
I
|
P
|
L
|
T
|
I
|
M
|
V
|
I
|
T
|
Y
|
254 |
F
|
L
|
T
|
I
|
K
|
S
|
L
|
Q
|
K
|
E
|
264 |
A
|
T
|
L
|
C
|
V
|
S
|
D
|
L
|
G
|
T
|
274 |
ICL3 | |||||||||
R
|
A
|
K
|
L
|
A
|
S
|
F
|
S
|
F
|
L
|
284 |
P
|
Q
|
S
|
S
|
L
|
S
|
S
|
E
|
K
|
L
|
294 |
F
|
Q
|
R
|
S
|
I
|
H
|
R
|
E
|
P
|
G
|
304 |
TM6 | |||||||||
S
|
Y
|
T
|
G
|
R
|
R
|
T
|
M
|
Q
|
S
|
314 |
I
|
S
|
N
|
E
|
Q
|
K
|
A
|
C
|
K
|
V
|
324 |
L
|
G
|
I
|
V
|
F
|
F
|
L
|
F
|
V
|
V
|
334 |
M
|
W
|
C
|
P
|
F
|
F
|
I
|
T
|
N
|
I
|
344 |
ECL3 | |||||||||
M
|
A
|
V
|
I
|
C
|
K
|
E
|
S
|
C
|
N
|
354 |
TM7 | |||||||||
E
|
D
|
V
|
I
|
G
|
A
|
L
|
L
|
N
|
V
|
364 |
F
|
V
|
W
|
I
|
G
|
Y
|
L
|
S
|
S
|
A
|
374 |
V
|
N
|
P
|
L
|
V
|
Y
|
T
|
L
|
F
|
N
|
384 |
H8 | |||||||||
K
|
T
|
Y
|
R
|
S
|
A
|
F
|
S
|
R
|
Y
|
394 |
|
|||||||||||||||||||||
C-term | |||||||||||||||||||||
I
|
Q
|
C
|
Q
|
Y
|
K
|
E
|
N
|
K
|
K
|
|
|||||||||||
404 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
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DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|