agrg1_human
NAME
7SF8
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
agrg1_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
|
T
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P
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Q
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S
|
L
|
L
|
Q
|
T
|
T
|
10 |
|
|
|
|
|
|
|
|
|
|
L
|
F
|
L
|
L
|
S
|
L
|
L
|
F
|
L
|
V
|
20 |
|
|
|
|
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|
|
|
|
|
Q
|
G
|
A
|
H
|
G
|
R
|
G
|
H
|
R
|
E
|
30 |
|
|
|
|
|
|
|
|
|
|
D
|
F
|
R
|
F
|
C
|
S
|
Q
|
R
|
N
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Q
|
40 |
|
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|
|
|
T
|
H
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R
|
S
|
S
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L
|
H
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Y
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K
|
P
|
50 |
|
|
|
|
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|
|
|
|
T
|
P
|
D
|
L
|
R
|
I
|
S
|
I
|
E
|
N
|
60 |
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|
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|
|
|
|
|
|
S
|
E
|
E
|
A
|
L
|
T
|
V
|
H
|
A
|
P
|
70 |
|
|
|
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|
|
|
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F
|
P
|
A
|
A
|
H
|
P
|
A
|
S
|
R
|
S
|
80 |
|
|
|
|
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|
|
|
|
|
F
|
P
|
D
|
P
|
R
|
G
|
L
|
Y
|
H
|
F
|
90 |
|
|
|
|
|
|
|
|
|
|
C
|
L
|
Y
|
W
|
N
|
R
|
H
|
A
|
G
|
R
|
100 |
|
|
|
|
|
|
|
|
|
|
L
|
H
|
L
|
L
|
Y
|
G
|
K
|
R
|
D
|
F
|
110 |
|
|
|
|
|
|
|
|
|
|
L
|
L
|
S
|
D
|
K
|
A
|
S
|
S
|
L
|
L
|
120 |
|
|
|
|
|
|
|
|
|
|
C
|
F
|
Q
|
H
|
Q
|
E
|
E
|
S
|
L
|
A
|
130 |
|
|
|
|
|
|
|
|
|
|
Q
|
G
|
P
|
P
|
L
|
L
|
A
|
T
|
S
|
V
|
140 |
|
|
|
|
|
|
|
|
|
|
T
|
S
|
W
|
W
|
S
|
P
|
Q
|
N
|
I
|
S
|
150 |
|
|
|
|
|
|
|
|
|
|
L
|
P
|
S
|
A
|
A
|
S
|
F
|
T
|
F
|
S
|
160 |
|
|
|
|
|
|
|
|
|
|
F
|
H
|
S
|
P
|
P
|
H
|
T
|
A
|
A
|
H
|
170 |
|
|
|
|
|
|
|
|
|
|
N
|
A
|
S
|
V
|
D
|
M
|
C
|
E
|
L
|
K
|
180 |
|
|
|
|
|
|
|
|
|
|
R
|
D
|
L
|
Q
|
L
|
L
|
S
|
Q
|
F
|
L
|
190 |
|
|
|
|
|
|
|
|
|
|
K
|
H
|
P
|
Q
|
K
|
A
|
S
|
R
|
R
|
P
|
200 |
|
|
|
|
|
|
|
|
|
|
S
|
A
|
A
|
P
|
A
|
S
|
Q
|
Q
|
L
|
Q
|
210 |
|
|
|
|
|
|
|
|
|
|
S
|
L
|
E
|
S
|
K
|
L
|
T
|
S
|
V
|
R
|
220 |
|
|
|
|
|
|
|
|
|
|
F
|
M
|
G
|
D
|
M
|
V
|
S
|
F
|
E
|
E
|
230 |
|
|
|
|
|
|
|
|
|
|
D
|
R
|
I
|
N
|
A
|
T
|
V
|
W
|
K
|
L
|
240 |
|
|
|
|
|
|
|
|
|
|
Q
|
P
|
T
|
A
|
G
|
L
|
Q
|
D
|
L
|
H
|
250 |
|
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|
|
|
|
|
|
|
|
I
|
H
|
S
|
R
|
Q
|
E
|
E
|
E
|
Q
|
S
|
260 |
|
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|
|
|
|
|
|
|
|
E
|
I
|
M
|
E
|
Y
|
S
|
V
|
L
|
L
|
P
|
270 |
|
|
|
|
|
|
|
|
|
|
R
|
T
|
L
|
F
|
Q
|
R
|
T
|
K
|
G
|
R
|
280 |
|
|
|
|
|
|
|
|
|
|
S
|
G
|
E
|
A
|
E
|
K
|
R
|
L
|
L
|
L
|
290 |
|
|
|
|
|
|
|
|
|
|
V
|
D
|
F
|
S
|
S
|
Q
|
A
|
L
|
F
|
Q
|
300 |
|
|
|
|
|
|
|
|
|
|
D
|
K
|
N
|
S
|
S
|
Q
|
V
|
L
|
G
|
E
|
310 |
|
|
|
|
|
|
|
|
|
|
K
|
V
|
L
|
G
|
I
|
V
|
V
|
Q
|
N
|
T
|
320 |
|
|
|
|
|
|
|
|
|
|
K
|
V
|
A
|
N
|
L
|
T
|
E
|
P
|
V
|
V
|
330 |
|
|
|
|
|
|
|
|
|
|
L
|
T
|
F
|
Q
|
H
|
Q
|
L
|
Q
|
P
|
K
|
340 |
|
|
|
|
|
|
|
|
|
|
N
|
V
|
T
|
L
|
Q
|
C
|
V
|
F
|
W
|
V
|
350 |
|
|
|
|
|
|
|
|
|
|
E
|
D
|
P
|
T
|
L
|
S
|
S
|
P
|
G
|
H
|
360 |
|
|
|
|
|
|
|
|
|
|
W
|
S
|
S
|
A
|
G
|
C
|
E
|
T
|
V
|
R
|
370 |
|
|
|
|
|
|
|
|
|
|
R
|
E
|
T
|
Q
|
T
|
S
|
C
|
F
|
C
|
N
|
380 |
|
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||||||||
H
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L
|
T
|
Y
|
F
|
A
|
V
|
L
|
M
|
V
|
390 |
TM1 | |||||||||
S
|
S
|
V
|
E
|
V
|
D
|
A
|
V
|
H
|
K
|
400 |
H
|
Y
|
L
|
S
|
L
|
L
|
S
|
Y
|
V
|
G
|
410 |
C
|
V
|
V
|
S
|
A
|
L
|
A
|
C
|
L
|
V
|
420 |
T
|
I
|
A
|
A
|
Y
|
L
|
C
|
S
|
R
|
V
|
430 |
ICL1 | |||||||||
P
|
L
|
P
|
C
|
R
|
R
|
K
|
P
|
R
|
D
|
440 |
TM2 | |||||||||
Y
|
T
|
I
|
K
|
V
|
H
|
M
|
N
|
L
|
L
|
450 |
L
|
A
|
V
|
F
|
L
|
L
|
D
|
T
|
S
|
F
|
460 |
L
|
L
|
S
|
E
|
P
|
V
|
A
|
L
|
T
|
G
|
470 |
TM3 | |||||||||
S
|
E
|
A
|
G
|
C
|
R
|
A
|
S
|
A
|
I
|
480 |
F
|
L
|
H
|
F
|
S
|
L
|
L
|
T
|
C
|
L
|
490 |
S
|
W
|
M
|
G
|
L
|
E
|
G
|
Y
|
N
|
L
|
500 |
ICL2 | |||||||||
Y
|
R
|
L
|
V
|
V
|
E
|
V
|
F
|
G
|
T
|
510 |
TM4 | |||||||||
Y
|
V
|
P
|
G
|
Y
|
L
|
L
|
K
|
L
|
S
|
520 |
A
|
M
|
G
|
W
|
G
|
F
|
P
|
I
|
F
|
L
|
530 |
V
|
T
|
L
|
V
|
A
|
L
|
V
|
D
|
V
|
D
|
540 |
ECL2 | |||||||||
N
|
Y
|
G
|
P
|
I
|
I
|
L
|
A
|
V
|
H
|
550 |
R
|
T
|
P
|
E
|
G
|
V
|
I
|
Y
|
P
|
S
|
560 |
TM5 | |||||||||
M
|
C
|
W
|
I
|
R
|
D
|
S
|
L
|
V
|
S
|
570 |
Y
|
I
|
T
|
N
|
L
|
G
|
L
|
F
|
S
|
L
|
580 |
V
|
F
|
L
|
F
|
N
|
M
|
A
|
M
|
L
|
A
|
590 |
T
|
M
|
V
|
V
|
Q
|
I
|
L
|
R
|
L
|
R
|
600 |
ICL3 | TM6 | ||||||||
P
|
H
|
T
|
Q
|
K
|
W
|
S
|
H
|
V
|
L
|
610 |
T
|
L
|
L
|
G
|
L
|
S
|
L
|
V
|
L
|
G
|
620 |
L
|
P
|
W
|
A
|
L
|
I
|
F
|
F
|
S
|
F
|
630 |
ECL3 | TM7 | ||||||||
A
|
S
|
G
|
T
|
F
|
Q
|
L
|
V
|
V
|
L
|
640 |
Y
|
L
|
F
|
S
|
I
|
I
|
T
|
S
|
F
|
Q
|
650 |
G
|
F
|
L
|
I
|
F
|
I
|
W
|
Y
|
W
|
S
|
660 |
C-term | |||||||||
M
|
R
|
L
|
Q
|
A
|
R
|
G
|
G
|
P
|
S
|
670 |
P
|
L
|
K
|
S
|
N
|
S
|
D
|
S
|
A
|
R
|
680 |
|
|
|
|||||||
L
|
P
|
I
|
S
|
S
|
G
|
S
|
T
|
S
|
S
|
690 |
|
|
|
S
|
R
|
I
|
|
|||||||||
N-term | |||||||||
|
T
|
Y
|
F
|
A
|
V
|
L
|
M
|
V
|
S
|
391 |
TM1 | |||||||||
S
|
V
|
E
|
V
|
D
|
A
|
V
|
H
|
K
|
H
|
401 |
Y
|
L
|
S
|
L
|
L
|
S
|
Y
|
V
|
G
|
C
|
411 |
V
|
V
|
S
|
A
|
L
|
A
|
C
|
L
|
V
|
T
|
421 |
I
|
A
|
A
|
Y
|
L
|
C
|
S
|
R
|
V
|
P
|
431 |
ICL1 | |||||||||
L
|
P
|
C
|
R
|
R
|
K
|
P
|
R
|
D
|
Y
|
441 |
TM2 | |||||||||
T
|
I
|
K
|
V
|
H
|
M
|
N
|
L
|
L
|
L
|
451 |
A
|
V
|
F
|
L
|
L
|
D
|
T
|
S
|
F
|
L
|
461 |
L
|
S
|
E
|
P
|
V
|
A
|
L
|
T
|
G
|
S
|
471 |
TM3 | |||||||||
E
|
A
|
G
|
C
|
R
|
A
|
S
|
A
|
I
|
F
|
481 |
L
|
H
|
F
|
S
|
L
|
L
|
T
|
C
|
L
|
S
|
491 |
W
|
M
|
G
|
L
|
E
|
G
|
Y
|
N
|
L
|
Y
|
501 |
ICL2 | |||||||||
R
|
L
|
V
|
V
|
E
|
V
|
F
|
G
|
T
|
Y
|
511 |
TM4 | |||||||||
V
|
P
|
G
|
Y
|
L
|
L
|
K
|
L
|
S
|
A
|
521 |
M
|
G
|
W
|
G
|
F
|
P
|
I
|
F
|
L
|
V
|
531 |
T
|
L
|
V
|
A
|
L
|
V
|
D
|
V
|
D
|
N
|
541 |
ECL2 | |||||||||
Y
|
G
|
P
|
I
|
I
|
L
|
A
|
V
|
H
|
R
|
551 |
T
|
P
|
E
|
G
|
V
|
I
|
Y
|
P
|
S
|
M
|
561 |
TM5 | |||||||||
C
|
W
|
I
|
R
|
D
|
S
|
L
|
V
|
S
|
Y
|
571 |
I
|
T
|
N
|
L
|
G
|
L
|
F
|
S
|
L
|
V
|
581 |
F
|
L
|
F
|
N
|
M
|
A
|
M
|
L
|
A
|
T
|
591 |
M
|
V
|
V
|
Q
|
I
|
L
|
R
|
L
|
R
|
P
|
601 |
ICL3 | TM6 | ||||||||
H
|
T
|
Q
|
K
|
W
|
S
|
H
|
V
|
L
|
T
|
611 |
L
|
L
|
G
|
L
|
S
|
L
|
V
|
L
|
G
|
L
|
621 |
P
|
W
|
A
|
L
|
I
|
F
|
F
|
S
|
F
|
A
|
631 |
TM7 | |||||||||
S
|
G
|
T
|
F
|
Q
|
L
|
V
|
V
|
L
|
Y
|
641 |
L
|
F
|
S
|
I
|
I
|
T
|
S
|
F
|
Q
|
G
|
651 |
F
|
L
|
I
|
F
|
I
|
W
|
Y
|
W
|
S
|
M
|
661 |
C-term | |||||||||
R
|
L
|
Q
|
A
|
R
|
G
|
G
|
P
|
S
|
P
|
671 |
L
|
K
|
S
|
N
|
S
|
D
|
S
|
A
|
R
|
L
|
681 |
|
||||||
P
|
I
|
S
|
S
|
G
|
S
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
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|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
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