calcr_human
NAME
7TYW
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
calcr_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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R
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F
|
T
|
F
|
T
|
S
|
R
|
C
|
L
|
10 |
|
|
|
|
|
|
|
|
|
|
A
|
L
|
F
|
L
|
L
|
L
|
N
|
H
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P
|
T
|
20 |
|
|
|
|
||||||
P
|
I
|
L
|
P
|
A
|
F
|
S
|
N
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Q
|
T
|
30 |
Y
|
P
|
T
|
I
|
E
|
P
|
K
|
P
|
F
|
L
|
40 |
Y
|
V
|
V
|
G
|
R
|
K
|
K
|
M
|
M
|
D
|
50 |
A
|
Q
|
Y
|
K
|
C
|
Y
|
D
|
R
|
M
|
Q
|
60 |
Q
|
L
|
P
|
A
|
Y
|
Q
|
G
|
E
|
G
|
P
|
70 |
Y
|
C
|
N
|
R
|
T
|
W
|
D
|
G
|
W
|
L
|
80 |
C
|
W
|
D
|
D
|
T
|
P
|
A
|
G
|
V
|
L
|
90 |
S
|
Y
|
Q
|
F
|
C
|
P
|
D
|
Y
|
F
|
P
|
100 |
D
|
F
|
D
|
P
|
S
|
E
|
K
|
V
|
T
|
K
|
110 |
Y
|
C
|
D
|
E
|
K
|
G
|
V
|
W
|
F
|
K
|
120 |
H
|
P
|
E
|
N
|
N
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R
|
T
|
W
|
S
|
N
|
130 |
TM1 | |||||||||
Y
|
T
|
M
|
C
|
N
|
A
|
F
|
T
|
P
|
E
|
140 |
K
|
L
|
K
|
N
|
A
|
Y
|
V
|
L
|
Y
|
Y
|
150 |
L
|
A
|
I
|
V
|
G
|
H
|
S
|
L
|
S
|
I
|
160 |
F
|
T
|
L
|
V
|
I
|
S
|
L
|
G
|
I
|
F
|
170 |
ICL1 | TM2 | ||||||||
V
|
F
|
F
|
R
|
S
|
L
|
G
|
C
|
Q
|
R
|
180 |
V
|
T
|
L
|
H
|
K
|
N
|
M
|
F
|
L
|
T
|
190 |
Y
|
I
|
L
|
N
|
S
|
M
|
I
|
I
|
I
|
I
|
200 |
ECL1 | |||||||||
H
|
L
|
V
|
E
|
V
|
V
|
P
|
N
|
G
|
E
|
210 |
TM3 | |||||||||
L
|
V
|
R
|
R
|
D
|
P
|
V
|
S
|
C
|
K
|
220 |
I
|
L
|
H
|
F
|
F
|
H
|
Q
|
Y
|
M
|
M
|
230 |
A
|
C
|
N
|
Y
|
F
|
W
|
M
|
L
|
C
|
E
|
240 |
G
|
I
|
Y
|
L
|
H
|
T
|
L
|
I
|
V
|
V
|
250 |
ICL2 | TM4 | ||||||||
A
|
V
|
F
|
T
|
E
|
K
|
Q
|
R
|
L
|
R
|
260 |
W
|
Y
|
Y
|
L
|
L
|
G
|
W
|
G
|
F
|
P
|
270 |
L
|
V
|
P
|
T
|
T
|
I
|
H
|
A
|
I
|
T
|
280 |
ECL2 | |||||||||
R
|
A
|
V
|
Y
|
F
|
N
|
D
|
N
|
C
|
W
|
290 |
TM5 | |||||||||
L
|
S
|
V
|
E
|
T
|
H
|
L
|
L
|
Y
|
I
|
300 |
I
|
H
|
G
|
P
|
V
|
M
|
A
|
A
|
L
|
V
|
310 |
V
|
N
|
F
|
F
|
F
|
L
|
L
|
N
|
I
|
V
|
320 |
R
|
V
|
L
|
V
|
T
|
K
|
M
|
R
|
E
|
T
|
330 |
ICL3 | TM6 | ||||||||
H
|
E
|
A
|
E
|
S
|
H
|
M
|
Y
|
L
|
K
|
340 |
A
|
V
|
K
|
A
|
T
|
M
|
I
|
L
|
V
|
P
|
350 |
L
|
L
|
G
|
I
|
Q
|
F
|
V
|
V
|
F
|
P
|
360 |
ECL3 | TM7 | ||||||||
W
|
R
|
P
|
S
|
N
|
K
|
M
|
L
|
G
|
K
|
370 |
I
|
Y
|
D
|
Y
|
V
|
M
|
H
|
S
|
L
|
I
|
380 |
H
|
F
|
Q
|
G
|
F
|
F
|
V
|
A
|
T
|
I
|
390 |
H8 | |||||||||
Y
|
C
|
F
|
C
|
N
|
N
|
E
|
V
|
Q
|
T
|
400 |
T
|
V
|
K
|
R
|
Q
|
W
|
A
|
Q
|
F
|
K
|
410 |
I
|
Q
|
W
|
N
|
Q
|
R
|
W
|
G
|
R
|
R
|
420 |
C-term | |||||||||
P
|
S
|
N
|
R
|
S
|
A
|
R
|
A
|
A
|
A
|
430 |
A
|
A
|
A
|
E
|
A
|
G
|
D
|
I
|
P
|
I
|
440 |
Y
|
I
|
C
|
H
|
Q
|
E
|
L
|
R
|
N
|
E
|
450 |
P
|
A
|
N
|
N
|
Q
|
G
|
E
|
E
|
S
|
A
|
460 |
E
|
I
|
I
|
P
|
L
|
N
|
I
|
I
|
E
|
Q
|
470 |
E
|
S
|
S
|
A
|
|
|||||||||
N-term | |||||||||
|
A
|
F
|
S
|
N
|
Q
|
T
|
Y
|
P
|
T
|
33 |
I
|
E
|
P
|
K
|
P
|
F
|
L
|
Y
|
V
|
V
|
43 |
G
|
R
|
K
|
K
|
M
|
M
|
D
|
A
|
Q
|
Y
|
53 |
K
|
C
|
Y
|
D
|
R
|
M
|
Q
|
Q
|
L
|
P
|
63 |
A
|
Y
|
Q
|
G
|
E
|
G
|
P
|
Y
|
C
|
N
|
73 |
R
|
T
|
W
|
D
|
G
|
W
|
L
|
C
|
W
|
D
|
83 |
D
|
T
|
P
|
A
|
G
|
V
|
L
|
S
|
Y
|
Q
|
93 |
F
|
C
|
P
|
D
|
Y
|
F
|
P
|
D
|
F
|
D
|
103 |
P
|
S
|
E
|
K
|
V
|
T
|
K
|
Y
|
C
|
D
|
113 |
E
|
K
|
G
|
V
|
W
|
F
|
K
|
H
|
P
|
E
|
123 |
N
|
N
|
R
|
T
|
W
|
S
|
N
|
Y
|
T
|
M
|
133 |
TM1 | |||||||||
C
|
N
|
A
|
F
|
T
|
P
|
E
|
K
|
L
|
K
|
143 |
N
|
A
|
Y
|
V
|
L
|
Y
|
Y
|
L
|
A
|
I
|
153 |
V
|
G
|
H
|
S
|
L
|
S
|
I
|
F
|
T
|
L
|
163 |
V
|
I
|
S
|
L
|
G
|
I
|
F
|
V
|
F
|
F
|
173 |
ICL1 | TM2 | ||||||||
R
|
S
|
L
|
G
|
C
|
Q
|
R
|
V
|
T
|
L
|
183 |
H
|
K
|
N
|
M
|
F
|
L
|
T
|
Y
|
I
|
L
|
193 |
N
|
S
|
M
|
I
|
I
|
I
|
I
|
H
|
L
|
V
|
203 |
ECL1 | |||||||||
E
|
V
|
V
|
P
|
N
|
G
|
E
|
L
|
V
|
R
|
213 |
TM3 | |||||||||
R
|
D
|
P
|
V
|
S
|
C
|
K
|
I
|
L
|
H
|
223 |
F
|
F
|
H
|
Q
|
Y
|
M
|
M
|
A
|
C
|
N
|
233 |
Y
|
F
|
W
|
M
|
L
|
C
|
E
|
G
|
I
|
Y
|
243 |
L
|
H
|
T
|
L
|
I
|
V
|
V
|
A
|
V
|
F
|
253 |
ICL2 | TM4 | ||||||||
T
|
E
|
K
|
Q
|
R
|
L
|
R
|
W
|
Y
|
Y
|
263 |
L
|
L
|
G
|
W
|
G
|
F
|
P
|
L
|
V
|
P
|
273 |
T
|
T
|
I
|
H
|
A
|
I
|
T
|
R
|
A
|
V
|
283 |
ECL2 | |||||||||
Y
|
F
|
N
|
D
|
N
|
C
|
W
|
L
|
S
|
V
|
293 |
TM5 | |||||||||
E
|
T
|
H
|
L
|
L
|
Y
|
I
|
I
|
H
|
G
|
303 |
P
|
V
|
M
|
A
|
A
|
L
|
V
|
V
|
N
|
F
|
313 |
F
|
F
|
L
|
L
|
N
|
I
|
V
|
R
|
V
|
L
|
323 |
V
|
T
|
K
|
M
|
R
|
E
|
T
|
H
|
E
|
A
|
333 |
TM6 | |||||||||
E
|
S
|
H
|
M
|
Y
|
L
|
K
|
A
|
V
|
K
|
343 |
A
|
T
|
M
|
I
|
L
|
V
|
P
|
L
|
L
|
G
|
353 |
I
|
Q
|
F
|
V
|
V
|
F
|
P
|
W
|
R
|
P
|
363 |
ECL3 | TM7 | ||||||||
S
|
N
|
K
|
M
|
L
|
G
|
K
|
I
|
Y
|
D
|
373 |
Y
|
V
|
M
|
H
|
S
|
L
|
I
|
H
|
F
|
Q
|
383 |
G
|
F
|
F
|
V
|
A
|
T
|
I
|
Y
|
C
|
F
|
393 |
H8 | |||||||||
C
|
N
|
N
|
E
|
V
|
Q
|
T
|
T
|
V
|
K
|
403 |
R
|
Q
|
W
|
A
|
Q
|
F
|
K
|
I
|
Q
|
W
|
413 |
N
|
Q
|
R
|
W
|
G
|
R
|
R
|
P
|
S
|
N
|
423 |
C-term | |||||||||
R
|
S
|
A
|
R
|
A
|
A
|
A
|
A
|
A
|
A
|
433 |
E
|
A
|
G
|
D
|
I
|
P
|
I
|
Y
|
I
|
C
|
443 |
H
|
Q
|
E
|
L
|
R
|
N
|
E
|
P
|
A
|
N
|
453 |
N
|
Q
|
G
|
E
|
E
|
S
|
A
|
E
|
I
|
I
|
463 |
P
|
L
|
N
|
I
|
I
|
E
|
Q
|
E
|
S
|
S
|
473 |
A
|
474 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
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|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|