grm3_human
NAME
7WIH
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
grm3_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
|
K
|
M
|
L
|
T
|
R
|
L
|
Q
|
V
|
L
|
10 |
|
|
|
|
|
|
|
|
|
|
T
|
L
|
A
|
L
|
F
|
S
|
K
|
G
|
F
|
L
|
20 |
|
|
||||||||
L
|
S
|
L
|
G
|
D
|
H
|
N
|
F
|
L
|
R
|
30 |
R
|
E
|
I
|
K
|
I
|
E
|
G
|
D
|
L
|
V
|
40 |
L
|
G
|
G
|
L
|
F
|
P
|
I
|
N
|
E
|
K
|
50 |
G
|
T
|
G
|
T
|
E
|
E
|
C
|
G
|
R
|
I
|
60 |
N
|
E
|
D
|
R
|
G
|
I
|
Q
|
R
|
L
|
E
|
70 |
A
|
M
|
L
|
F
|
A
|
I
|
D
|
E
|
I
|
N
|
80 |
K
|
D
|
D
|
Y
|
L
|
L
|
P
|
G
|
V
|
K
|
90 |
L
|
G
|
V
|
H
|
I
|
L
|
D
|
T
|
C
|
S
|
100 |
R
|
D
|
T
|
Y
|
A
|
L
|
E
|
Q
|
S
|
L
|
110 |
E
|
F
|
V
|
R
|
A
|
S
|
L
|
T
|
K
|
V
|
120 |
D
|
E
|
A
|
E
|
Y
|
M
|
C
|
P
|
D
|
G
|
130 |
S
|
Y
|
A
|
I
|
Q
|
E
|
N
|
I
|
P
|
L
|
140 |
L
|
I
|
A
|
G
|
V
|
I
|
G
|
G
|
S
|
Y
|
150 |
S
|
S
|
V
|
S
|
I
|
Q
|
V
|
A
|
N
|
L
|
160 |
L
|
R
|
L
|
F
|
Q
|
I
|
P
|
Q
|
I
|
S
|
170 |
Y
|
A
|
S
|
T
|
S
|
A
|
K
|
L
|
S
|
D
|
180 |
K
|
S
|
R
|
Y
|
D
|
Y
|
F
|
A
|
R
|
T
|
190 |
V
|
P
|
P
|
D
|
F
|
Y
|
Q
|
A
|
K
|
A
|
200 |
M
|
A
|
E
|
I
|
L
|
R
|
F
|
F
|
N
|
W
|
210 |
T
|
Y
|
V
|
S
|
T
|
V
|
A
|
S
|
E
|
G
|
220 |
D
|
Y
|
G
|
E
|
T
|
G
|
I
|
E
|
A
|
F
|
230 |
E
|
Q
|
E
|
A
|
R
|
L
|
R
|
N
|
I
|
C
|
240 |
I
|
A
|
T
|
A
|
E
|
K
|
V
|
G
|
R
|
S
|
250 |
N
|
I
|
R
|
K
|
S
|
Y
|
D
|
S
|
V
|
I
|
260 |
R
|
E
|
L
|
L
|
Q
|
K
|
P
|
N
|
A
|
R
|
270 |
V
|
V
|
V
|
L
|
F
|
M
|
R
|
S
|
D
|
D
|
280 |
S
|
R
|
E
|
L
|
I
|
A
|
A
|
A
|
S
|
R
|
290 |
A
|
N
|
A
|
S
|
F
|
T
|
W
|
V
|
A
|
S
|
300 |
D
|
G
|
W
|
G
|
A
|
Q
|
E
|
S
|
I
|
I
|
310 |
K
|
G
|
S
|
E
|
H
|
V
|
A
|
Y
|
G
|
A
|
320 |
I
|
T
|
L
|
E
|
L
|
A
|
S
|
Q
|
P
|
V
|
330 |
R
|
Q
|
F
|
D
|
R
|
Y
|
F
|
Q
|
S
|
L
|
340 |
N
|
P
|
Y
|
N
|
N
|
H
|
R
|
N
|
P
|
W
|
350 |
F
|
R
|
D
|
F
|
W
|
E
|
Q
|
K
|
F
|
Q
|
360 |
C
|
S
|
L
|
Q
|
N
|
K
|
R
|
N
|
H
|
R
|
370 |
R
|
V
|
C
|
D
|
K
|
H
|
L
|
A
|
I
|
D
|
380 |
S
|
S
|
N
|
Y
|
E
|
Q
|
E
|
S
|
K
|
I
|
390 |
M
|
F
|
V
|
V
|
N
|
A
|
V
|
Y
|
A
|
M
|
400 |
A
|
H
|
A
|
L
|
H
|
K
|
M
|
Q
|
R
|
T
|
410 |
L
|
C
|
P
|
N
|
T
|
T
|
K
|
L
|
C
|
D
|
420 |
A
|
M
|
K
|
I
|
L
|
D
|
G
|
K
|
K
|
L
|
430 |
Y
|
K
|
D
|
Y
|
L
|
L
|
K
|
I
|
N
|
F
|
440 |
T
|
A
|
P
|
F
|
N
|
P
|
N
|
K
|
D
|
A
|
450 |
D
|
S
|
I
|
V
|
K
|
F
|
D
|
T
|
F
|
G
|
460 |
D
|
G
|
M
|
G
|
R
|
Y
|
N
|
V
|
F
|
N
|
470 |
F
|
Q
|
N
|
V
|
G
|
G
|
K
|
Y
|
S
|
Y
|
480 |
L
|
K
|
V
|
G
|
H
|
W
|
A
|
E
|
T
|
L
|
490 |
S
|
L
|
D
|
V
|
N
|
S
|
I
|
H
|
W
|
S
|
500 |
R
|
N
|
S
|
V
|
P
|
T
|
S
|
Q
|
C
|
S
|
510 |
D
|
P
|
C
|
A
|
P
|
N
|
E
|
M
|
K
|
N
|
520 |
M
|
Q
|
P
|
G
|
D
|
V
|
C
|
C
|
W
|
I
|
530 |
C
|
I
|
P
|
C
|
E
|
P
|
Y
|
E
|
Y
|
L
|
540 |
A
|
D
|
E
|
F
|
T
|
C
|
M
|
D
|
C
|
G
|
550 |
S
|
G
|
Q
|
W
|
P
|
T
|
A
|
D
|
L
|
T
|
560 |
G
|
C
|
Y
|
D
|
L
|
P
|
E
|
D
|
Y
|
I
|
570 |
TM1 | |||||||||
R
|
W
|
E
|
D
|
A
|
W
|
A
|
I
|
G
|
P
|
580 |
V
|
T
|
I
|
A
|
C
|
L
|
G
|
F
|
M
|
C
|
590 |
T
|
C
|
M
|
V
|
V
|
T
|
V
|
F
|
I
|
K
|
600 |
ICL1 | |||||||||
H
|
N
|
N
|
T
|
P
|
L
|
V
|
K
|
A
|
S
|
610 |
TM2 | |||||||||
G
|
R
|
E
|
L
|
C
|
Y
|
I
|
L
|
L
|
F
|
620 |
G
|
V
|
G
|
L
|
S
|
Y
|
C
|
M
|
T
|
F
|
630 |
ECL1 | TM3 | ||||||||
F
|
F
|
I
|
A
|
K
|
P
|
S
|
P
|
V
|
I
|
640 |
C
|
A
|
L
|
R
|
R
|
L
|
G
|
L
|
G
|
S
|
650 |
S
|
F
|
A
|
I
|
C
|
Y
|
S
|
A
|
L
|
L
|
660 |
T
|
K
|
T
|
N
|
C
|
I
|
A
|
R
|
I
|
F
|
670 |
ICL2 | |||||||||
D
|
G
|
V
|
K
|
N
|
G
|
A
|
Q
|
R
|
P
|
680 |
TM4 | |||||||||
K
|
F
|
I
|
S
|
P
|
S
|
S
|
Q
|
V
|
F
|
690 |
I
|
C
|
L
|
G
|
L
|
I
|
L
|
V
|
Q
|
I
|
700 |
V
|
M
|
V
|
S
|
V
|
W
|
L
|
I
|
L
|
E
|
710 |
ECL2 | |||||||||
A
|
P
|
G
|
T
|
R
|
R
|
Y
|
T
|
L
|
A
|
720 |
E
|
K
|
R
|
E
|
T
|
V
|
I
|
L
|
K
|
C
|
730 |
TM5 | |||||||||
N
|
V
|
K
|
D
|
S
|
S
|
M
|
L
|
I
|
S
|
740 |
L
|
T
|
Y
|
D
|
V
|
I
|
L
|
V
|
I
|
L
|
750 |
C
|
T
|
V
|
Y
|
A
|
F
|
K
|
T
|
R
|
K
|
760 |
ICL3 | TM6 | ||||||||
C
|
P
|
E
|
N
|
F
|
N
|
E
|
A
|
K
|
F
|
770 |
I
|
G
|
F
|
T
|
M
|
Y
|
T
|
T
|
C
|
I
|
780 |
I
|
W
|
L
|
A
|
F
|
L
|
P
|
I
|
F
|
Y
|
790 |
ECL3 | TM7 | ||||||||
V
|
T
|
S
|
S
|
D
|
Y
|
R
|
V
|
Q
|
T
|
800 |
T
|
T
|
M
|
C
|
I
|
S
|
V
|
S
|
L
|
S
|
810 |
G
|
F
|
V
|
V
|
L
|
G
|
C
|
L
|
F
|
A
|
820 |
P
|
K
|
V
|
H
|
I
|
I
|
L
|
F
|
Q
|
P
|
830 |
H8 | |||||||||
Q
|
K
|
N
|
V
|
V
|
T
|
H
|
R
|
L
|
H
|
840 |
C-term | |||||||||
L
|
N
|
R
|
F
|
S
|
V
|
S
|
G
|
T
|
G
|
850 |
T
|
T
|
Y
|
S
|
Q
|
S
|
S
|
A
|
S
|
T
|
860 |
Y
|
V
|
P
|
T
|
V
|
C
|
N
|
G
|
R
|
E
|
870 |
V
|
L
|
D
|
S
|
T
|
T
|
S
|
S
|
L
|
|
|||||||||
N-term | |||||||||
|
L
|
G
|
D
|
H
|
N
|
F
|
L
|
R
|
R
|
31 |
E
|
I
|
K
|
I
|
E
|
G
|
D
|
L
|
V
|
L
|
41 |
G
|
G
|
L
|
F
|
P
|
I
|
N
|
E
|
K
|
G
|
51 |
T
|
G
|
T
|
E
|
E
|
C
|
G
|
R
|
I
|
N
|
61 |
E
|
D
|
R
|
G
|
I
|
Q
|
R
|
L
|
E
|
A
|
71 |
M
|
L
|
F
|
A
|
I
|
D
|
E
|
I
|
N
|
K
|
81 |
D
|
D
|
Y
|
L
|
L
|
P
|
G
|
V
|
K
|
L
|
91 |
G
|
V
|
H
|
I
|
L
|
D
|
T
|
C
|
S
|
R
|
101 |
D
|
T
|
Y
|
A
|
L
|
E
|
Q
|
S
|
L
|
E
|
111 |
F
|
V
|
R
|
A
|
S
|
L
|
T
|
K
|
V
|
D
|
121 |
E
|
A
|
E
|
Y
|
M
|
C
|
P
|
D
|
G
|
S
|
131 |
Y
|
A
|
I
|
Q
|
E
|
N
|
I
|
P
|
L
|
L
|
141 |
I
|
A
|
G
|
V
|
I
|
G
|
G
|
S
|
Y
|
S
|
151 |
S
|
V
|
S
|
I
|
Q
|
V
|
A
|
N
|
L
|
L
|
161 |
R
|
L
|
F
|
Q
|
I
|
P
|
Q
|
I
|
S
|
Y
|
171 |
A
|
S
|
T
|
S
|
A
|
K
|
L
|
S
|
D
|
K
|
181 |
S
|
R
|
Y
|
D
|
Y
|
F
|
A
|
R
|
T
|
V
|
191 |
P
|
P
|
D
|
F
|
Y
|
Q
|
A
|
K
|
A
|
M
|
201 |
A
|
E
|
I
|
L
|
R
|
F
|
F
|
N
|
W
|
T
|
211 |
Y
|
V
|
S
|
T
|
V
|
A
|
S
|
E
|
G
|
D
|
221 |
Y
|
G
|
E
|
T
|
G
|
I
|
E
|
A
|
F
|
E
|
231 |
Q
|
E
|
A
|
R
|
L
|
R
|
N
|
I
|
C
|
I
|
241 |
A
|
T
|
A
|
E
|
K
|
V
|
G
|
R
|
S
|
N
|
251 |
I
|
R
|
K
|
S
|
Y
|
D
|
S
|
V
|
I
|
R
|
261 |
E
|
L
|
L
|
Q
|
K
|
P
|
N
|
A
|
R
|
V
|
271 |
V
|
V
|
L
|
F
|
M
|
R
|
S
|
D
|
D
|
S
|
281 |
R
|
E
|
L
|
I
|
A
|
A
|
A
|
S
|
R
|
A
|
291 |
N
|
A
|
S
|
F
|
T
|
W
|
V
|
A
|
S
|
D
|
301 |
G
|
W
|
G
|
A
|
Q
|
E
|
S
|
I
|
I
|
K
|
311 |
G
|
S
|
E
|
H
|
V
|
A
|
Y
|
G
|
A
|
I
|
321 |
T
|
L
|
E
|
L
|
A
|
S
|
Q
|
P
|
V
|
R
|
331 |
Q
|
F
|
D
|
R
|
Y
|
F
|
Q
|
S
|
L
|
N
|
341 |
P
|
Y
|
N
|
N
|
H
|
R
|
N
|
P
|
W
|
F
|
351 |
R
|
D
|
F
|
W
|
E
|
Q
|
K
|
F
|
Q
|
C
|
361 |
S
|
L
|
Q
|
N
|
K
|
R
|
N
|
H
|
R
|
R
|
371 |
V
|
C
|
D
|
K
|
H
|
L
|
A
|
I
|
D
|
S
|
381 |
S
|
N
|
Y
|
E
|
Q
|
E
|
S
|
K
|
I
|
M
|
391 |
F
|
V
|
V
|
N
|
A
|
V
|
Y
|
A
|
M
|
A
|
401 |
H
|
A
|
L
|
H
|
K
|
M
|
Q
|
R
|
T
|
L
|
411 |
C
|
P
|
N
|
T
|
T
|
K
|
L
|
C
|
D
|
A
|
421 |
M
|
K
|
I
|
L
|
D
|
G
|
K
|
K
|
L
|
Y
|
431 |
K
|
D
|
Y
|
L
|
L
|
K
|
I
|
N
|
F
|
T
|
441 |
A
|
P
|
F
|
N
|
P
|
N
|
K
|
D
|
A
|
D
|
451 |
S
|
I
|
V
|
K
|
F
|
D
|
T
|
F
|
G
|
D
|
461 |
G
|
M
|
G
|
R
|
Y
|
N
|
V
|
F
|
N
|
F
|
471 |
Q
|
N
|
V
|
G
|
G
|
K
|
Y
|
S
|
Y
|
L
|
481 |
K
|
V
|
G
|
H
|
W
|
A
|
E
|
T
|
L
|
S
|
491 |
L
|
D
|
V
|
N
|
S
|
I
|
H
|
W
|
S
|
R
|
501 |
N
|
S
|
V
|
P
|
T
|
S
|
Q
|
C
|
S
|
D
|
511 |
P
|
C
|
A
|
P
|
N
|
E
|
M
|
K
|
N
|
M
|
521 |
Q
|
P
|
G
|
D
|
V
|
C
|
C
|
W
|
I
|
C
|
531 |
I
|
P
|
C
|
E
|
P
|
Y
|
E
|
Y
|
L
|
A
|
541 |
D
|
E
|
F
|
T
|
C
|
M
|
D
|
C
|
G
|
S
|
551 |
G
|
Q
|
W
|
P
|
T
|
A
|
D
|
L
|
T
|
G
|
561 |
C
|
Y
|
D
|
L
|
P
|
E
|
D
|
Y
|
I
|
R
|
571 |
TM1 | |||||||||
W
|
E
|
D
|
A
|
W
|
A
|
I
|
G
|
P
|
V
|
581 |
T
|
I
|
A
|
C
|
L
|
G
|
F
|
M
|
C
|
T
|
591 |
C
|
M
|
V
|
V
|
T
|
V
|
F
|
I
|
K
|
H
|
601 |
ICL1 | |||||||||
N
|
N
|
T
|
P
|
L
|
V
|
K
|
A
|
S
|
G
|
611 |
TM2 | |||||||||
R
|
E
|
L
|
C
|
Y
|
I
|
L
|
L
|
F
|
G
|
621 |
V
|
G
|
L
|
S
|
Y
|
C
|
M
|
T
|
F
|
F
|
631 |
ECL1 | TM3 | ||||||||
F
|
I
|
A
|
K
|
P
|
S
|
P
|
V
|
I
|
C
|
641 |
A
|
L
|
R
|
R
|
L
|
G
|
L
|
G
|
S
|
S
|
651 |
F
|
A
|
I
|
C
|
Y
|
S
|
A
|
L
|
L
|
T
|
661 |
K
|
T
|
N
|
C
|
I
|
A
|
R
|
I
|
F
|
D
|
671 |
ICL2 | |||||||||
G
|
V
|
K
|
N
|
G
|
A
|
Q
|
R
|
P
|
K
|
681 |
TM4 | |||||||||
F
|
I
|
S
|
P
|
S
|
S
|
Q
|
V
|
F
|
I
|
691 |
C
|
L
|
G
|
L
|
I
|
L
|
V
|
Q
|
I
|
V
|
701 |
M
|
V
|
S
|
V
|
W
|
L
|
I
|
L
|
E
|
A
|
711 |
ECL2 | |||||||||
P
|
G
|
T
|
R
|
R
|
Y
|
T
|
L
|
A
|
E
|
721 |
K
|
R
|
E
|
T
|
V
|
I
|
L
|
K
|
C
|
N
|
731 |
TM5 | |||||||||
V
|
K
|
D
|
S
|
S
|
M
|
L
|
I
|
S
|
L
|
741 |
T
|
Y
|
D
|
V
|
I
|
L
|
V
|
I
|
L
|
C
|
751 |
T
|
V
|
Y
|
A
|
F
|
K
|
T
|
R
|
K
|
C
|
761 |
ICL3 | TM6 | ||||||||
P
|
E
|
N
|
F
|
N
|
E
|
A
|
K
|
F
|
I
|
771 |
G
|
F
|
T
|
M
|
Y
|
T
|
T
|
C
|
I
|
I
|
781 |
W
|
L
|
A
|
F
|
L
|
P
|
I
|
F
|
Y
|
V
|
791 |
ECL3 | TM7 | ||||||||
T
|
S
|
S
|
D
|
Y
|
R
|
V
|
Q
|
T
|
T
|
801 |
T
|
M
|
C
|
I
|
S
|
V
|
S
|
L
|
S
|
G
|
811 |
F
|
V
|
V
|
L
|
G
|
C
|
L
|
F
|
A
|
P
|
821 |
H8 | |||||||||
K
|
V
|
H
|
I
|
I
|
L
|
F
|
Q
|
P
|
Q
|
831 |
K
|
N
|
V
|
V
|
T
|
H
|
R
|
L
|
H
|
L
|
841 |
C-term | |||||||||
N
|
R
|
F
|
S
|
V
|
S
|
G
|
T
|
G
|
T
|
851 |
T
|
Y
|
S
|
Q
|
S
|
S
|
A
|
S
|
T
|
Y
|
861 |
V
|
P
|
T
|
V
|
C
|
N
|
G
|
R
|
E
|
V
|
871 |
L
|
D
|
S
|
T
|
T
|
S
|
S
|
L
|
879 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
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|
|
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|
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|
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|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|