agrg2_mouse
NAME
7WUQ
ORGANISM
Mouse (Mus musculus)
PROTEIN NAME
agrg2_mouse
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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L
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F
|
S
|
G
|
G
|
Q
|
Y
|
S
|
P
|
10 |
|
|
|
|
|
|
|
|
|
|
V
|
G
|
R
|
P
|
E
|
E
|
V
|
L
|
L
|
I
|
20 |
|
|
|
|
|
|
|
|
|
|
Y
|
K
|
I
|
F
|
L
|
V
|
I
|
I
|
C
|
F
|
30 |
|
|
|
|
|
|
|
|
|
|
H
|
V
|
I
|
L
|
V
|
T
|
S
|
L
|
K
|
E
|
40 |
|
|
|
|
|
|
|
|
|
|
N
|
G
|
N
|
S
|
S
|
L
|
L
|
S
|
P
|
S
|
50 |
|
|
|
|
|
|
|
|
|
|
A
|
E
|
S
|
S
|
L
|
V
|
S
|
L
|
I
|
P
|
60 |
|
|
|
|
|
|
|
|
|
|
Y
|
S
|
N
|
G
|
T
|
P
|
D
|
A
|
A
|
S
|
70 |
|
|
|
|
|
|
|
|
|
|
E
|
V
|
L
|
S
|
T
|
L
|
N
|
K
|
T
|
E
|
80 |
|
|
|
|
|
|
|
|
|
|
K
|
S
|
K
|
I
|
T
|
I
|
V
|
K
|
T
|
F
|
90 |
|
|
|
|
|
|
|
|
|
|
N
|
A
|
S
|
G
|
V
|
K
|
S
|
Q
|
R
|
N
|
100 |
|
|
|
|
|
|
|
|
|
|
I
|
C
|
N
|
L
|
S
|
S
|
L
|
C
|
N
|
D
|
110 |
|
|
|
|
|
|
|
|
|
|
S
|
V
|
F
|
F
|
R
|
G
|
E
|
I
|
V
|
F
|
120 |
|
|
|
|
|
|
|
|
|
|
Q
|
H
|
D
|
E
|
D
|
H
|
N
|
V
|
T
|
Q
|
130 |
|
|
|
|
|
|
|
|
|
|
N
|
Q
|
D
|
T
|
A
|
N
|
G
|
T
|
F
|
A
|
140 |
|
|
|
|
|
|
|
|
|
|
G
|
V
|
L
|
S
|
L
|
S
|
E
|
L
|
K
|
R
|
150 |
|
|
|
|
|
|
|
|
|
|
S
|
E
|
L
|
N
|
K
|
T
|
L
|
Q
|
T
|
L
|
160 |
|
|
|
|
|
|
|
|
|
|
S
|
E
|
T
|
Y
|
F
|
I
|
V
|
C
|
A
|
T
|
170 |
|
|
|
|
|
|
|
|
|
|
A
|
E
|
A
|
Q
|
S
|
T
|
V
|
N
|
C
|
T
|
180 |
|
|
|
|
|
|
|
|
|
|
F
|
T
|
V
|
K
|
L
|
N
|
E
|
T
|
M
|
N
|
190 |
|
|
|
|
|
|
|
|
|
|
V
|
C
|
A
|
M
|
M
|
V
|
T
|
F
|
Q
|
T
|
200 |
|
|
|
|
|
|
|
|
|
|
V
|
Q
|
I
|
R
|
P
|
M
|
E
|
Q
|
C
|
C
|
210 |
|
|
|
|
|
|
|
|
|
|
C
|
S
|
P
|
R
|
T
|
P
|
C
|
P
|
S
|
S
|
220 |
|
|
|
|
|
|
|
|
|
|
P
|
E
|
E
|
L
|
E
|
K
|
L
|
Q
|
C
|
E
|
230 |
|
|
|
|
|
|
|
|
|
|
L
|
Q
|
D
|
P
|
I
|
V
|
C
|
L
|
A
|
D
|
240 |
|
|
|
|
|
|
|
|
|
|
Q
|
P
|
H
|
G
|
P
|
P
|
L
|
S
|
S
|
S
|
250 |
|
|
|
|
|
|
|
|
|
|
S
|
K
|
P
|
V
|
V
|
P
|
Q
|
A
|
T
|
I
|
260 |
|
|
|
|
|
|
|
|
|
|
I
|
S
|
H
|
V
|
A
|
S
|
D
|
F
|
S
|
L
|
270 |
|
|
|
|
|
|
|
|
|
|
A
|
E
|
P
|
L
|
D
|
H
|
A
|
L
|
M
|
T
|
280 |
|
|
|
|
|
|
|
|
|
|
P
|
S
|
T
|
P
|
S
|
L
|
T
|
Q
|
E
|
S
|
290 |
|
|
|
|
|
|
|
|
|
|
N
|
L
|
P
|
S
|
P
|
Q
|
P
|
T
|
I
|
P
|
300 |
|
|
|
|
|
|
|
|
|
|
L
|
A
|
S
|
S
|
P
|
A
|
T
|
D
|
L
|
P
|
310 |
|
|
|
|
|
|
|
|
|
|
V
|
Q
|
S
|
V
|
V
|
V
|
S
|
S
|
L
|
P
|
320 |
|
|
|
|
|
|
|
|
|
|
Q
|
T
|
D
|
L
|
S
|
H
|
T
|
L
|
S
|
P
|
330 |
|
|
|
|
|
|
|
|
|
|
V
|
Q
|
S
|
S
|
I
|
P
|
S
|
P
|
T
|
T
|
340 |
|
|
|
|
|
|
|
|
|
|
P
|
A
|
P
|
S
|
V
|
P
|
T
|
E
|
L
|
V
|
350 |
|
|
|
|
|
|
|
|
|
|
T
|
I
|
S
|
T
|
P
|
P
|
G
|
E
|
T
|
V
|
360 |
|
|
|
|
|
|
|
|
|
|
V
|
N
|
T
|
S
|
T
|
V
|
S
|
D
|
L
|
E
|
370 |
|
|
|
|
|
|
|
|
|
|
A
|
Q
|
V
|
S
|
Q
|
M
|
E
|
K
|
A
|
L
|
380 |
|
|
|
|
|
|
|
|
|
|
S
|
L
|
G
|
S
|
L
|
E
|
P
|
N
|
L
|
A
|
390 |
|
|
|
|
|
|
|
|
|
|
G
|
E
|
M
|
V
|
N
|
R
|
V
|
S
|
K
|
L
|
400 |
|
|
|
|
|
|
|
|
|
|
L
|
H
|
S
|
P
|
P
|
A
|
L
|
L
|
A
|
P
|
410 |
|
|
|
|
|
|
|
|
|
|
L
|
A
|
Q
|
R
|
L
|
L
|
K
|
V
|
V
|
D
|
420 |
|
|
|
|
|
|
|
|
|
|
A
|
I
|
G
|
L
|
Q
|
L
|
N
|
F
|
S
|
S
|
430 |
|
|
|
|
|
|
|
|
|
|
T
|
T
|
I
|
S
|
L
|
T
|
S
|
P
|
S
|
L
|
440 |
|
|
|
|
|
|
|
|
|
|
A
|
L
|
A
|
V
|
I
|
R
|
V
|
N
|
A
|
S
|
450 |
|
|
|
|
|
|
|
|
|
|
N
|
F
|
N
|
T
|
T
|
T
|
F
|
A
|
A
|
Q
|
460 |
|
|
|
|
|
|
|
|
|
|
D
|
P
|
T
|
N
|
L
|
Q
|
V
|
S
|
L
|
E
|
470 |
|
|
|
|
|
|
|
|
|
|
T
|
P
|
P
|
P
|
E
|
N
|
S
|
I
|
G
|
A
|
480 |
|
|
|
|
|
|
|
|
|
|
I
|
T
|
L
|
P
|
S
|
S
|
L
|
M
|
N
|
N
|
490 |
|
|
|
|
|
|
|
|
|
|
L
|
P
|
A
|
N
|
D
|
V
|
E
|
L
|
A
|
S
|
500 |
|
|
|
|
|
|
|
|
|
|
R
|
I
|
Q
|
F
|
N
|
F
|
F
|
E
|
T
|
P
|
510 |
|
|
|
|
|
|
|
|
|
|
A
|
L
|
F
|
Q
|
D
|
P
|
S
|
L
|
E
|
N
|
520 |
|
|
|
|
|
|
|
|
|
|
L
|
T
|
L
|
I
|
S
|
Y
|
V
|
I
|
S
|
S
|
530 |
|
|
|
|
|
|
|
|
|
|
S
|
V
|
T
|
N
|
M
|
T
|
I
|
K
|
N
|
L
|
540 |
|
|
|
|
|
|
|
|
|
|
T
|
R
|
N
|
V
|
T
|
V
|
A
|
L
|
K
|
H
|
550 |
|
|
|
|
|
|
|
|
|
|
I
|
N
|
P
|
S
|
P
|
D
|
D
|
L
|
T
|
V
|
560 |
|
|
|
|
|
|
|
|
|
|
K
|
C
|
V
|
F
|
W
|
D
|
L
|
G
|
R
|
N
|
570 |
|
|
|
|
|
|
|
|
|
|
G
|
G
|
K
|
G
|
G
|
W
|
S
|
S
|
D
|
G
|
580 |
|
|
|
|
|
|
|
|
|
|
C
|
S
|
V
|
K
|
D
|
K
|
R
|
M
|
N
|
E
|
590 |
|
|
|
|
|
|
||||
T
|
I
|
C
|
T
|
C
|
S
|
H
|
L
|
T
|
S
|
600 |
F
|
G
|
I
|
L
|
L
|
D
|
L
|
S
|
R
|
T
|
610 |
TM1 | |||||||||
S
|
L
|
P
|
P
|
S
|
Q
|
M
|
M
|
A
|
L
|
620 |
T
|
F
|
I
|
T
|
Y
|
I
|
G
|
C
|
G
|
L
|
630 |
S
|
S
|
I
|
F
|
L
|
S
|
V
|
T
|
L
|
V
|
640 |
ICL1 | |||||||||
T
|
Y
|
I
|
A
|
F
|
E
|
K
|
I
|
R
|
R
|
650 |
TM2 | |||||||||
D
|
Y
|
P
|
S
|
K
|
I
|
L
|
I
|
Q
|
L
|
660 |
C
|
A
|
A
|
L
|
L
|
L
|
L
|
N
|
L
|
I
|
670 |
F
|
L
|
L
|
D
|
S
|
W
|
I
|
A
|
L
|
Y
|
680 |
ECL1 | TM3 | ||||||||
N
|
T
|
R
|
G
|
F
|
C
|
I
|
A
|
V
|
A
|
690 |
V
|
F
|
L
|
H
|
Y
|
F
|
L
|
L
|
V
|
S
|
700 |
F
|
T
|
W
|
M
|
G
|
L
|
E
|
A
|
F
|
H
|
710 |
M
|
Y
|
L
|
A
|
L
|
V
|
K
|
V
|
F
|
N
|
720 |
ICL2 | TM4 | ||||||||
T
|
Y
|
I
|
R
|
K
|
Y
|
I
|
L
|
K
|
F
|
730 |
C
|
I
|
V
|
G
|
W
|
G
|
I
|
P
|
A
|
V
|
740 |
V
|
V
|
S
|
I
|
V
|
L
|
T
|
I
|
S
|
P
|
750 |
ECL2 | |||||||||
D
|
N
|
Y
|
G
|
I
|
G
|
S
|
Y
|
G
|
K
|
760 |
F
|
P
|
N
|
G
|
T
|
P
|
D
|
D
|
F
|
C
|
770 |
TM5 | |||||||||
W
|
I
|
N
|
S
|
N
|
V
|
V
|
F
|
Y
|
I
|
780 |
T
|
V
|
V
|
G
|
Y
|
F
|
C
|
V
|
I
|
F
|
790 |
L
|
L
|
N
|
V
|
S
|
M
|
F
|
I
|
V
|
V
|
800 |
L
|
V
|
Q
|
L
|
C
|
R
|
I
|
K
|
K
|
K
|
810 |
ICL3 | |||||||||
K
|
Q
|
L
|
G
|
A
|
Q
|
R
|
K
|
T
|
S
|
820 |
TM6 | |||||||||
I
|
Q
|
D
|
L
|
R
|
S
|
I
|
A
|
G
|
L
|
830 |
T
|
F
|
L
|
L
|
G
|
I
|
T
|
W
|
G
|
F
|
840 |
ECL3 | |||||||||
A
|
F
|
F
|
A
|
W
|
G
|
P
|
V
|
N
|
V
|
850 |
TM7 | |||||||||
T
|
F
|
M
|
Y
|
L
|
F
|
A
|
I
|
F
|
N
|
860 |
T
|
L
|
Q
|
G
|
F
|
F
|
I
|
F
|
I
|
F
|
870 |
H8 | |||||||||
Y
|
C
|
A
|
A
|
K
|
E
|
N
|
V
|
R
|
K
|
880 |
Q
|
W
|
R
|
R
|
Y
|
L
|
C
|
C
|
G
|
K
|
890 |
C-term | |||||||||
L
|
R
|
L
|
A
|
E
|
N
|
S
|
D
|
W
|
S
|
900 |
K
|
T
|
A
|
T
|
N
|
G
|
L
|
K
|
K
|
Q
|
910 |
T
|
V
|
N
|
Q
|
G
|
V
|
S
|
S
|
S
|
S
|
920 |
N
|
S
|
L
|
Q
|
S
|
S
|
C
|
N
|
S
|
T
|
930 |
N
|
S
|
T
|
T
|
L
|
L
|
V
|
N
|
S
|
D
|
940 |
C
|
S
|
V
|
H
|
A
|
S
|
G
|
N
|
G
|
N
|
950 |
A
|
S
|
T
|
E
|
R
|
N
|
G
|
V
|
S
|
F
|
960 |
S
|
V
|
Q
|
N
|
G
|
D
|
V
|
C
|
L
|
H
|
970 |
D
|
L
|
T
|
G
|
K
|
Q
|
H
|
M
|
F
|
S
|
980 |
D
|
K
|
E
|
D
|
S
|
C
|
N
|
G
|
K
|
S
|
990 |
R
|
I
|
A
|
L
|
R
|
R
|
T
|
S
|
K
|
R
|
1000 |
G
|
S
|
L
|
H
|
F
|
I
|
E
|
Q
|
M
|
|
|||||||||
N-term | |||||||||
|
H
|
L
|
T
|
S
|
F
|
G
|
I
|
L
|
L
|
605 |
D
|
L
|
S
|
R
|
T
|
S
|
L
|
P
|
P
|
S
|
615 |
TM1 | |||||||||
Q
|
M
|
M
|
A
|
L
|
T
|
F
|
I
|
T
|
Y
|
625 |
I
|
G
|
C
|
G
|
L
|
S
|
S
|
I
|
F
|
L
|
635 |
S
|
V
|
T
|
L
|
V
|
T
|
Y
|
I
|
A
|
F
|
645 |
ICL1 | TM2 | ||||||||
E
|
K
|
I
|
R
|
R
|
D
|
Y
|
P
|
S
|
K
|
655 |
I
|
L
|
I
|
Q
|
L
|
C
|
A
|
A
|
L
|
L
|
665 |
L
|
L
|
N
|
L
|
I
|
F
|
L
|
L
|
D
|
S
|
675 |
ECL1 | TM3 | ||||||||||
W
|
I
|
A
|
L
|
Y
|
N
|
T
|
R
|
G
|
F
|
||
685 |
C
|
I
|
A
|
V
|
A
|
V
|
F
|
L
|
H
|
Y
|
695 |
F
|
L
|
L
|
V
|
S
|
F
|
T
|
W
|
M
|
G
|
705 |
L
|
E
|
A
|
F
|
H
|
M
|
Y
|
L
|
A
|
L
|
715 |
ICL2 | |||||||||
V
|
K
|
V
|
F
|
N
|
T
|
Y
|
I
|
R
|
K
|
725 |
TM4 | |||||||||
Y
|
I
|
L
|
K
|
F
|
C
|
I
|
V
|
G
|
W
|
735 |
G
|
I
|
P
|
A
|
V
|
V
|
V
|
S
|
I
|
V
|
745 |
ECL2 | |||||||||
L
|
T
|
I
|
S
|
P
|
D
|
N
|
Y
|
G
|
I
|
755 |
G
|
S
|
Y
|
G
|
K
|
F
|
P
|
N
|
G
|
T
|
765 |
P
|
D
|
D
|
F
|
C
|
W
|
I
|
N
|
S
|
N
|
775 |
TM5 | |||||||||
V
|
V
|
F
|
Y
|
I
|
T
|
V
|
V
|
G
|
Y
|
785 |
F
|
C
|
V
|
I
|
F
|
L
|
L
|
N
|
V
|
S
|
795 |
M
|
F
|
I
|
V
|
V
|
L
|
V
|
Q
|
L
|
C
|
805 |
ICL3 | |||||||||
R
|
I
|
K
|
K
|
K
|
K
|
Q
|
L
|
G
|
A
|
815 |
TM6 | |||||||||
Q
|
R
|
K
|
T
|
S
|
I
|
Q
|
D
|
L
|
R
|
825 |
S
|
I
|
A
|
G
|
L
|
T
|
F
|
L
|
L
|
G
|
835 |
I
|
T
|
W
|
G
|
F
|
A
|
F
|
F
|
A
|
W
|
845 |
ECL3 | TM7 | ||||||||
G
|
P
|
V
|
N
|
V
|
T
|
F
|
M
|
Y
|
L
|
855 |
F
|
A
|
I
|
F
|
N
|
T
|
L
|
Q
|
G
|
F
|
865 |
F
|
I
|
F
|
I
|
F
|
Y
|
C
|
A
|
A
|
K
|
875 |
H8 | |||||||||
E
|
N
|
V
|
R
|
K
|
Q
|
W
|
R
|
R
|
Y
|
885 |
L
|
C
|
C
|
G
|
K
|
L
|
R
|
L
|
A
|
E
|
895 |
C-term | |||||||||
N
|
S
|
D
|
W
|
S
|
K
|
T
|
A
|
T
|
N
|
905 |
G
|
L
|
K
|
K
|
Q
|
T
|
V
|
N
|
Q
|
G
|
915 |
V
|
S
|
S
|
S
|
S
|
N
|
S
|
L
|
Q
|
S
|
925 |
S
|
C
|
N
|
S
|
T
|
N
|
S
|
T
|
T
|
L
|
935 |
L
|
V
|
N
|
S
|
D
|
C
|
S
|
V
|
H
|
A
|
945 |
S
|
G
|
N
|
G
|
N
|
A
|
S
|
T
|
E
|
R
|
955 |
N
|
G
|
V
|
S
|
F
|
S
|
V
|
Q
|
N
|
G
|
965 |
D
|
V
|
C
|
L
|
H
|
D
|
L
|
T
|
G
|
K
|
975 |
Q
|
H
|
M
|
F
|
S
|
D
|
K
|
E
|
D
|
S
|
985 |
C
|
N
|
G
|
K
|
S
|
R
|
I
|
A
|
L
|
R
|
995 |
R
|
T
|
S
|
K
|
R
|
G
|
S
|
L
|
H
|
F
|
1005 |
I
|
E
|
Q
|
M
|
1009 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|