agrl3_mouse
NAME
7WY5
ORGANISM
Mouse (Mus musculus)
PROTEIN NAME
agrl3_mouse
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
N-term | |||||||||
M
|
W
|
P
|
P
|
Q
|
L
|
L
|
I
|
L
|
T
|
10 |
M
|
L
|
L
|
A
|
P
|
V
|
V
|
H
|
G
|
G
|
20 |
K
|
H
|
N
|
E
|
R
|
H
|
P
|
A
|
L
|
A
|
30 |
A
|
P
|
L
|
R
|
H
|
A
|
E
|
R
|
S
|
P
|
40 |
G
|
G
|
A
|
L
|
P
|
P
|
R
|
H
|
L
|
L
|
50 |
Q
|
Q
|
P
|
A
|
A
|
E
|
R
|
S
|
T
|
A
|
60 |
H
|
R
|
G
|
Q
|
G
|
P
|
R
|
G
|
A
|
A
|
70 |
R
|
G
|
V
|
R
|
G
|
P
|
G
|
A
|
P
|
G
|
80 |
A
|
Q
|
I
|
A
|
A
|
Q
|
A
|
F
|
S
|
R
|
90 |
A
|
P
|
I
|
P
|
M
|
A
|
V
|
V
|
R
|
R
|
100 |
E
|
L
|
S
|
C
|
E
|
S
|
Y
|
P
|
I
|
E
|
110 |
L
|
R
|
C
|
P
|
G
|
T
|
D
|
V
|
I
|
M
|
120 |
I
|
E
|
S
|
A
|
N
|
Y
|
G
|
R
|
T
|
D
|
130 |
D
|
K
|
I
|
C
|
D
|
S
|
D
|
P
|
A
|
Q
|
140 |
M
|
E
|
N
|
I
|
R
|
C
|
Y
|
L
|
P
|
D
|
150 |
A
|
Y
|
K
|
I
|
M
|
S
|
Q
|
R
|
C
|
N
|
160 |
N
|
R
|
T
|
Q
|
C
|
A
|
V
|
V
|
A
|
G
|
170 |
P
|
D
|
V
|
F
|
P
|
D
|
P
|
C
|
P
|
G
|
180 |
T
|
Y
|
K
|
Y
|
L
|
E
|
V
|
Q
|
Y
|
E
|
190 |
C
|
V
|
P
|
Y
|
K
|
V
|
E
|
Q
|
K
|
V
|
200 |
F
|
L
|
C
|
P
|
G
|
L
|
L
|
K
|
G
|
V
|
210 |
Y
|
Q
|
S
|
E
|
H
|
L
|
F
|
E
|
S
|
D
|
220 |
H
|
Q
|
S
|
G
|
A
|
W
|
C
|
K
|
D
|
P
|
230 |
L
|
Q
|
A
|
S
|
D
|
K
|
I
|
Y
|
Y
|
M
|
240 |
P
|
W
|
T
|
P
|
Y
|
R
|
T
|
D
|
T
|
L
|
250 |
T
|
E
|
Y
|
S
|
S
|
K
|
D
|
D
|
F
|
I
|
260 |
A
|
G
|
R
|
P
|
T
|
T
|
T
|
Y
|
K
|
L
|
270 |
P
|
H
|
R
|
V
|
D
|
G
|
T
|
G
|
F
|
V
|
280 |
V
|
Y
|
D
|
G
|
A
|
L
|
F
|
F
|
N
|
K
|
290 |
E
|
R
|
T
|
R
|
N
|
I
|
V
|
K
|
F
|
D
|
300 |
L
|
R
|
T
|
R
|
I
|
K
|
S
|
G
|
E
|
A
|
310 |
I
|
I
|
A
|
N
|
A
|
N
|
Y
|
H
|
D
|
T
|
320 |
S
|
P
|
Y
|
R
|
W
|
G
|
G
|
K
|
S
|
D
|
330 |
I
|
D
|
L
|
A
|
V
|
D
|
E
|
N
|
G
|
L
|
340 |
W
|
V
|
I
|
Y
|
A
|
T
|
E
|
Q
|
N
|
N
|
350 |
G
|
K
|
I
|
V
|
I
|
S
|
Q
|
L
|
N
|
P
|
360 |
Y
|
T
|
L
|
R
|
I
|
E
|
G
|
T
|
W
|
D
|
370 |
T
|
A
|
Y
|
D
|
K
|
R
|
S
|
A
|
S
|
N
|
380 |
A
|
F
|
M
|
I
|
C
|
G
|
I
|
L
|
Y
|
V
|
390 |
V
|
K
|
S
|
V
|
Y
|
E
|
D
|
D
|
D
|
N
|
400 |
E
|
A
|
T
|
G
|
N
|
K
|
I
|
D
|
Y
|
I
|
410 |
Y
|
N
|
T
|
D
|
Q
|
S
|
K
|
D
|
S
|
L
|
420 |
V
|
D
|
V
|
P
|
F
|
P
|
N
|
S
|
Y
|
Q
|
430 |
Y
|
I
|
A
|
A
|
V
|
D
|
Y
|
N
|
P
|
R
|
440 |
D
|
N
|
L
|
L
|
Y
|
V
|
W
|
N
|
N
|
Y
|
450 |
H
|
V
|
V
|
K
|
Y
|
S
|
L
|
D
|
F
|
G
|
460 |
P
|
L
|
D
|
S
|
R
|
S
|
G
|
P
|
V
|
H
|
470 |
H
|
G
|
Q
|
V
|
S
|
Y
|
I
|
S
|
P
|
P
|
480 |
I
|
H
|
L
|
D
|
S
|
E
|
L
|
E
|
R
|
P
|
490 |
P
|
V
|
R
|
G
|
I
|
S
|
T
|
T
|
G
|
S
|
500 |
L
|
G
|
M
|
G
|
S
|
T
|
T
|
T
|
S
|
T
|
510 |
T
|
L
|
R
|
T
|
T
|
T
|
W
|
N
|
I
|
G
|
520 |
R
|
S
|
T
|
T
|
A
|
S
|
L
|
P
|
G
|
R
|
530 |
R
|
N
|
R
|
S
|
T
|
S
|
T
|
P
|
S
|
P
|
540 |
A
|
V
|
E
|
V
|
L
|
D
|
D
|
V
|
T
|
T
|
550 |
H
|
L
|
P
|
S
|
A
|
A
|
S
|
Q
|
I
|
P
|
560 |
A
|
M
|
E
|
E
|
S
|
C
|
E
|
A
|
V
|
E
|
570 |
A
|
R
|
E
|
I
|
M
|
W
|
F
|
K
|
T
|
R
|
580 |
Q
|
G
|
Q
|
V
|
A
|
K
|
Q
|
P
|
C
|
P
|
590 |
A
|
G
|
T
|
I
|
G
|
V
|
S
|
T
|
Y
|
L
|
600 |
C
|
L
|
A
|
P
|
D
|
G
|
I
|
W
|
D
|
P
|
610 |
Q
|
G
|
P
|
D
|
L
|
S
|
N
|
C
|
S
|
S
|
620 |
P
|
W
|
V
|
N
|
H
|
I
|
T
|
Q
|
K
|
L
|
630 |
K
|
S
|
G
|
E
|
T
|
A
|
A
|
N
|
I
|
A
|
640 |
R
|
E
|
L
|
A
|
E
|
Q
|
T
|
R
|
N
|
H
|
650 |
L
|
N
|
A
|
G
|
D
|
I
|
T
|
Y
|
S
|
V
|
660 |
R
|
A
|
M
|
D
|
Q
|
L
|
V
|
G
|
L
|
L
|
670 |
D
|
V
|
Q
|
L
|
R
|
N
|
L
|
T
|
P
|
G
|
680 |
G
|
K
|
D
|
S
|
A
|
A
|
R
|
S
|
L
|
N
|
690 |
K
|
L
|
Q
|
K
|
R
|
E
|
R
|
S
|
C
|
R
|
700 |
A
|
Y
|
V
|
Q
|
A
|
M
|
V
|
E
|
T
|
V
|
710 |
N
|
N
|
L
|
L
|
Q
|
P
|
Q
|
A
|
L
|
N
|
720 |
A
|
W
|
R
|
D
|
L
|
T
|
T
|
S
|
D
|
Q
|
730 |
L
|
R
|
A
|
A
|
T
|
M
|
L
|
L
|
D
|
T
|
740 |
V
|
E
|
E
|
S
|
A
|
F
|
V
|
L
|
A
|
D
|
750 |
N
|
L
|
L
|
K
|
T
|
D
|
I
|
V
|
R
|
E
|
760 |
N
|
T
|
D
|
N
|
I
|
Q
|
L
|
E
|
V
|
A
|
770 |
R
|
L
|
S
|
T
|
E
|
G
|
N
|
L
|
E
|
D
|
780 |
L
|
K
|
F
|
P
|
E
|
N
|
M
|
G
|
H
|
G
|
790 |
S
|
T
|
I
|
Q
|
L
|
S
|
A
|
N
|
T
|
L
|
800 |
K
|
Q
|
N
|
G
|
R
|
N
|
G
|
E
|
I
|
R
|
810 |
V
|
A
|
F
|
V
|
L
|
Y
|
N
|
N
|
L
|
G
|
820 |
P
|
Y
|
L
|
S
|
T
|
E
|
N
|
A
|
S
|
M
|
830 |
K
|
L
|
G
|
T
|
E
|
A
|
M
|
S
|
T
|
N
|
840 |
H
|
S
|
V
|
I
|
V
|
N
|
S
|
P
|
V
|
I
|
850 |
T
|
A
|
A
|
I
|
N
|
K
|
E
|
F
|
S
|
N
|
860 |
K
|
V
|
Y
|
L
|
A
|
D
|
P
|
V
|
V
|
F
|
870 |
T
|
V
|
K
|
H
|
I
|
K
|
Q
|
S
|
E
|
E
|
880 |
N
|
F
|
N
|
P
|
N
|
C
|
S
|
F
|
W
|
S
|
890 |
Y
|
S
|
K
|
R
|
T
|
M
|
T
|
G
|
Y
|
W
|
900 |
S
|
T
|
Q
|
G
|
C
|
R
|
L
|
L
|
T
|
T
|
910 |
N
|
K
|
T
|
H
|
T
|
T
|
C
|
S
|
C
|
N
|
920 |
H
|
L
|
T
|
N
|
F
|
A
|
V
|
L
|
M
|
A
|
930 |
H
|
V
|
E
|
V
|
K
|
H
|
S
|
D
|
A
|
V
|
940 |
TM1 | |||||||||
H
|
D
|
L
|
L
|
L
|
D
|
V
|
I
|
T
|
W
|
950 |
V
|
G
|
I
|
L
|
L
|
S
|
L
|
V
|
C
|
L
|
960 |
L
|
I
|
C
|
I
|
F
|
T
|
F
|
C
|
F
|
F
|
970 |
ICL1 | TM2 | ||||||||
R
|
G
|
L
|
Q
|
S
|
D
|
R
|
N
|
T
|
I
|
980 |
H
|
K
|
N
|
L
|
C
|
I
|
S
|
L
|
F
|
V
|
990 |
A
|
E
|
L
|
L
|
F
|
L
|
I
|
G
|
I
|
N
|
1000 |
ECL1 | TM3 | ||||||||
R
|
T
|
D
|
Q
|
P
|
I
|
A
|
C
|
A
|
V
|
1010 |
F
|
A
|
A
|
L
|
L
|
H
|
F
|
F
|
F
|
L
|
1020 |
A
|
A
|
F
|
T
|
W
|
M
|
F
|
L
|
E
|
G
|
1030 |
V
|
Q
|
L
|
Y
|
I
|
M
|
L
|
V
|
E
|
V
|
1040 |
ICL2 | TM4 | ||||||||
F
|
E
|
S
|
E
|
H
|
S
|
R
|
R
|
K
|
Y
|
1050 |
F
|
Y
|
L
|
V
|
G
|
Y
|
G
|
M
|
P
|
A
|
1060 |
L
|
I
|
V
|
A
|
V
|
S
|
A
|
A
|
V
|
D
|
1070 |
ECL2 | |||||||||
Y
|
R
|
S
|
Y
|
G
|
T
|
D
|
K
|
V
|
C
|
1080 |
TM5 | |||||||||
W
|
L
|
R
|
L
|
D
|
T
|
Y
|
F
|
I
|
W
|
1090 |
S
|
F
|
I
|
G
|
P
|
A
|
T
|
L
|
I
|
I
|
1100 |
M
|
L
|
N
|
V
|
I
|
F
|
L
|
G
|
I
|
A
|
1110 |
L
|
Y
|
K
|
M
|
F
|
H
|
H
|
T
|
A
|
I
|
1120 |
ICL3 | |||||||||
L
|
K
|
P
|
E
|
S
|
G
|
C
|
L
|
D
|
N
|
1130 |
TM6 | |||||||||
I
|
N
|
Y
|
E
|
D
|
N
|
R
|
P
|
F
|
I
|
1140 |
K
|
S
|
W
|
V
|
I
|
G
|
A
|
I
|
A
|
L
|
1150 |
L
|
C
|
L
|
L
|
G
|
L
|
T
|
W
|
A
|
F
|
1160 |
ECL3 | |||||||||
G
|
L
|
M
|
Y
|
I
|
N
|
E
|
S
|
T
|
V
|
1170 |
TM7 | |||||||||
I
|
M
|
A
|
Y
|
L
|
F
|
T
|
I
|
F
|
N
|
1180 |
S
|
L
|
Q
|
G
|
M
|
F
|
I
|
F
|
I
|
F
|
1190 |
H8 | |||||||||
H
|
C
|
V
|
L
|
Q
|
K
|
K
|
V
|
R
|
K
|
1200 |
E
|
Y
|
G
|
K
|
C
|
L
|
R
|
T
|
H
|
C
|
1210 |
C-term | |||||||||
C
|
S
|
G
|
K
|
S
|
T
|
E
|
S
|
S
|
I
|
1220 |
G
|
S
|
G
|
K
|
T
|
S
|
G
|
S
|
R
|
T
|
1230 |
P
|
G
|
R
|
Y
|
S
|
T
|
G
|
S
|
Q
|
S
|
1240 |
R
|
I
|
R
|
R
|
M
|
W
|
N
|
D
|
T
|
V
|
1250 |
R
|
K
|
Q
|
S
|
E
|
S
|
S
|
F
|
I
|
T
|
1260 |
G
|
D
|
I
|
N
|
S
|
S
|
A
|
S
|
L
|
N
|
1270 |
R
|
E
|
G
|
L
|
L
|
N
|
N
|
A
|
R
|
D
|
1280 |
T
|
S
|
V
|
M
|
D
|
T
|
L
|
P
|
L
|
N
|
1290 |
G
|
N
|
H
|
G
|
N
|
S
|
Y
|
S
|
I
|
A
|
1300 |
G
|
G
|
E
|
Y
|
L
|
S
|
N
|
C
|
V
|
Q
|
1310 |
I
|
I
|
D
|
R
|
G
|
Y
|
N
|
H
|
N
|
E
|
1320 |
T
|
A
|
L
|
E
|
K
|
K
|
I
|
L
|
K
|
E
|
1330 |
L
|
T
|
S
|
N
|
Y
|
I
|
P
|
S
|
Y
|
L
|
1340 |
N
|
N
|
H
|
E
|
R
|
S
|
S
|
E
|
Q
|
N
|
1350 |
R
|
N
|
M
|
M
|
N
|
K
|
L
|
V
|
N
|
N
|
1360 |
L
|
G
|
S
|
G
|
S
|
E
|
D
|
D
|
A
|
I
|
1370 |
V
|
L
|
D
|
D
|
A
|
A
|
S
|
F
|
N
|
H
|
1380 |
E
|
E
|
S
|
L
|
G
|
L
|
E
|
L
|
I
|
H
|
1390 |
E
|
E
|
S
|
D
|
A
|
P
|
L
|
L
|
P
|
P
|
1400 |
R
|
V
|
Y
|
S
|
T
|
D
|
N
|
H
|
Q
|
P
|
1410 |
H
|
H
|
Y
|
S
|
R
|
R
|
R
|
F
|
P
|
Q
|
1420 |
D
|
H
|
S
|
E
|
S
|
F
|
F
|
P
|
L
|
L
|
1430 |
T
|
D
|
E
|
H
|
T
|
E
|
D
|
L
|
Q
|
S
|
1440 |
P
|
H
|
R
|
D
|
S
|
L
|
Y
|
T
|
S
|
M
|
1450 |
P
|
A
|
L
|
A
|
G
|
V
|
P
|
A
|
A
|
D
|
1460 |
S
|
V
|
T
|
T
|
S
|
T
|
Q
|
T
|
E
|
A
|
1470 |
A
|
A
|
A
|
K
|
G
|
G
|
D
|
A
|
E
|
D
|
1480 |
V
|
Y
|
Y
|
K
|
S
|
M
|
P
|
N
|
L
|
G
|
1490 |
S
|
R
|
N
|
H
|
V
|
H
|
P
|
L
|
H
|
A
|
1500 |
Y
|
Y
|
Q
|
L
|
G
|
R
|
G
|
S
|
S
|
D
|
1510 |
G
|
F
|
I
|
V
|
P
|
P
|
N
|
K
|
D
|
G
|
1520 |
A
|
S
|
P
|
E
|
G
|
T
|
S
|
K
|
G
|
P
|
1530 |
A
|
H
|
L
|
V
|
T
|
S
|
L
|
N-term | |||||||||
M
|
W
|
P
|
P
|
Q
|
L
|
L
|
I
|
L
|
T
|
10 |
M
|
L
|
L
|
A
|
P
|
V
|
V
|
H
|
G
|
G
|
20 |
K
|
H
|
N
|
E
|
R
|
H
|
P
|
A
|
L
|
A
|
30 |
A
|
P
|
L
|
R
|
H
|
A
|
E
|
R
|
S
|
P
|
40 |
G
|
G
|
A
|
L
|
P
|
P
|
R
|
H
|
L
|
L
|
50 |
Q
|
Q
|
P
|
A
|
A
|
E
|
R
|
S
|
T
|
A
|
60 |
H
|
R
|
G
|
Q
|
G
|
P
|
R
|
G
|
A
|
A
|
70 |
R
|
G
|
V
|
R
|
G
|
P
|
G
|
A
|
P
|
G
|
80 |
A
|
Q
|
I
|
A
|
A
|
Q
|
A
|
F
|
S
|
R
|
90 |
A
|
P
|
I
|
P
|
M
|
A
|
V
|
V
|
R
|
R
|
100 |
E
|
L
|
S
|
C
|
E
|
S
|
Y
|
P
|
I
|
E
|
110 |
L
|
R
|
C
|
P
|
G
|
T
|
D
|
V
|
I
|
M
|
120 |
I
|
E
|
S
|
A
|
N
|
Y
|
G
|
R
|
T
|
D
|
130 |
D
|
K
|
I
|
C
|
D
|
S
|
D
|
P
|
A
|
Q
|
140 |
M
|
E
|
N
|
I
|
R
|
C
|
Y
|
L
|
P
|
D
|
150 |
A
|
Y
|
K
|
I
|
M
|
S
|
Q
|
R
|
C
|
N
|
160 |
N
|
R
|
T
|
Q
|
C
|
A
|
V
|
V
|
A
|
G
|
170 |
P
|
D
|
V
|
F
|
P
|
D
|
P
|
C
|
P
|
G
|
180 |
T
|
Y
|
K
|
Y
|
L
|
E
|
V
|
Q
|
Y
|
E
|
190 |
C
|
V
|
P
|
Y
|
K
|
V
|
E
|
Q
|
K
|
V
|
200 |
F
|
L
|
C
|
P
|
G
|
L
|
L
|
K
|
G
|
V
|
210 |
Y
|
Q
|
S
|
E
|
H
|
L
|
F
|
E
|
S
|
D
|
220 |
H
|
Q
|
S
|
G
|
A
|
W
|
C
|
K
|
D
|
P
|
230 |
L
|
Q
|
A
|
S
|
D
|
K
|
I
|
Y
|
Y
|
M
|
240 |
P
|
W
|
T
|
P
|
Y
|
R
|
T
|
D
|
T
|
L
|
250 |
T
|
E
|
Y
|
S
|
S
|
K
|
D
|
D
|
F
|
I
|
260 |
A
|
G
|
R
|
P
|
T
|
T
|
T
|
Y
|
K
|
L
|
270 |
P
|
H
|
R
|
V
|
D
|
G
|
T
|
G
|
F
|
V
|
280 |
V
|
Y
|
D
|
G
|
A
|
L
|
F
|
F
|
N
|
K
|
290 |
E
|
R
|
T
|
R
|
N
|
I
|
V
|
K
|
F
|
D
|
300 |
L
|
R
|
T
|
R
|
I
|
K
|
S
|
G
|
E
|
A
|
310 |
I
|
I
|
A
|
N
|
A
|
N
|
Y
|
H
|
D
|
T
|
320 |
S
|
P
|
Y
|
R
|
W
|
G
|
G
|
K
|
S
|
D
|
330 |
I
|
D
|
L
|
A
|
V
|
D
|
E
|
N
|
G
|
L
|
340 |
W
|
V
|
I
|
Y
|
A
|
T
|
E
|
Q
|
N
|
N
|
350 |
G
|
K
|
I
|
V
|
I
|
S
|
Q
|
L
|
N
|
P
|
360 |
Y
|
T
|
L
|
R
|
I
|
E
|
G
|
T
|
W
|
D
|
370 |
T
|
A
|
Y
|
D
|
K
|
R
|
S
|
A
|
S
|
N
|
380 |
A
|
F
|
M
|
I
|
C
|
G
|
I
|
L
|
Y
|
V
|
390 |
V
|
K
|
S
|
V
|
Y
|
E
|
D
|
D
|
D
|
N
|
400 |
E
|
A
|
T
|
G
|
N
|
K
|
I
|
D
|
Y
|
I
|
410 |
Y
|
N
|
T
|
D
|
Q
|
S
|
K
|
D
|
S
|
L
|
420 |
V
|
D
|
V
|
P
|
F
|
P
|
N
|
S
|
Y
|
Q
|
430 |
Y
|
I
|
A
|
A
|
V
|
D
|
Y
|
N
|
P
|
R
|
440 |
D
|
N
|
L
|
L
|
Y
|
V
|
W
|
N
|
N
|
Y
|
450 |
H
|
V
|
V
|
K
|
Y
|
S
|
L
|
D
|
F
|
G
|
460 |
P
|
L
|
D
|
S
|
R
|
S
|
G
|
P
|
V
|
H
|
470 |
H
|
G
|
Q
|
V
|
S
|
Y
|
I
|
S
|
P
|
P
|
480 |
I
|
H
|
L
|
D
|
S
|
E
|
L
|
E
|
R
|
P
|
490 |
P
|
V
|
R
|
G
|
I
|
S
|
T
|
T
|
G
|
S
|
500 |
L
|
G
|
M
|
G
|
S
|
T
|
T
|
T
|
S
|
T
|
510 |
T
|
L
|
R
|
T
|
T
|
T
|
W
|
N
|
I
|
G
|
520 |
R
|
S
|
T
|
T
|
A
|
S
|
L
|
P
|
G
|
R
|
530 |
R
|
N
|
R
|
S
|
T
|
S
|
T
|
P
|
S
|
P
|
540 |
A
|
V
|
E
|
V
|
L
|
D
|
D
|
V
|
T
|
T
|
550 |
H
|
L
|
P
|
S
|
A
|
A
|
S
|
Q
|
I
|
P
|
560 |
A
|
M
|
E
|
E
|
S
|
C
|
E
|
A
|
V
|
E
|
570 |
A
|
R
|
E
|
I
|
M
|
W
|
F
|
K
|
T
|
R
|
580 |
Q
|
G
|
Q
|
V
|
A
|
K
|
Q
|
P
|
C
|
P
|
590 |
A
|
G
|
T
|
I
|
G
|
V
|
S
|
T
|
Y
|
L
|
600 |
C
|
L
|
A
|
P
|
D
|
G
|
I
|
W
|
D
|
P
|
610 |
Q
|
G
|
P
|
D
|
L
|
S
|
N
|
C
|
S
|
S
|
620 |
P
|
W
|
V
|
N
|
H
|
I
|
T
|
Q
|
K
|
L
|
630 |
K
|
S
|
G
|
E
|
T
|
A
|
A
|
N
|
I
|
A
|
640 |
R
|
E
|
L
|
A
|
E
|
Q
|
T
|
R
|
N
|
H
|
650 |
L
|
N
|
A
|
G
|
D
|
I
|
T
|
Y
|
S
|
V
|
660 |
R
|
A
|
M
|
D
|
Q
|
L
|
V
|
G
|
L
|
L
|
670 |
D
|
V
|
Q
|
L
|
R
|
N
|
L
|
T
|
P
|
G
|
680 |
G
|
K
|
D
|
S
|
A
|
A
|
R
|
S
|
L
|
N
|
690 |
K
|
L
|
Q
|
K
|
R
|
E
|
R
|
S
|
C
|
R
|
700 |
A
|
Y
|
V
|
Q
|
A
|
M
|
V
|
E
|
T
|
V
|
710 |
N
|
N
|
L
|
L
|
Q
|
P
|
Q
|
A
|
L
|
N
|
720 |
A
|
W
|
R
|
D
|
L
|
T
|
T
|
S
|
D
|
Q
|
730 |
L
|
R
|
A
|
A
|
T
|
M
|
L
|
L
|
D
|
T
|
740 |
V
|
E
|
E
|
S
|
A
|
F
|
V
|
L
|
A
|
D
|
750 |
N
|
L
|
L
|
K
|
T
|
D
|
I
|
V
|
R
|
E
|
760 |
N
|
T
|
D
|
N
|
I
|
Q
|
L
|
E
|
V
|
A
|
770 |
R
|
L
|
S
|
T
|
E
|
G
|
N
|
L
|
E
|
D
|
780 |
L
|
K
|
F
|
P
|
E
|
N
|
M
|
G
|
H
|
G
|
790 |
S
|
T
|
I
|
Q
|
L
|
S
|
A
|
N
|
T
|
L
|
800 |
K
|
Q
|
N
|
G
|
R
|
N
|
G
|
E
|
I
|
R
|
810 |
V
|
A
|
F
|
V
|
L
|
Y
|
N
|
N
|
L
|
G
|
820 |
P
|
Y
|
L
|
S
|
T
|
E
|
N
|
A
|
S
|
M
|
830 |
K
|
L
|
G
|
T
|
E
|
A
|
M
|
S
|
T
|
N
|
840 |
H
|
S
|
V
|
I
|
V
|
N
|
S
|
P
|
V
|
I
|
850 |
T
|
A
|
A
|
I
|
N
|
K
|
E
|
F
|
S
|
N
|
860 |
K
|
V
|
Y
|
L
|
A
|
D
|
P
|
V
|
V
|
F
|
870 |
T
|
V
|
K
|
H
|
I
|
K
|
Q
|
S
|
E
|
E
|
880 |
N
|
F
|
N
|
P
|
N
|
C
|
S
|
F
|
W
|
S
|
890 |
Y
|
S
|
K
|
R
|
T
|
M
|
T
|
G
|
Y
|
W
|
900 |
S
|
T
|
Q
|
G
|
C
|
R
|
L
|
L
|
T
|
T
|
910 |
N
|
K
|
T
|
H
|
T
|
T
|
C
|
S
|
C
|
N
|
920 |
H
|
L
|
T
|
N
|
F
|
A
|
V
|
L
|
M
|
A
|
930 |
H
|
V
|
E
|
V
|
K
|
H
|
S
|
D
|
A
|
V
|
940 |
TM1 | |||||||||
H
|
D
|
L
|
L
|
L
|
D
|
V
|
I
|
T
|
W
|
950 |
V
|
G
|
I
|
L
|
L
|
S
|
L
|
V
|
C
|
L
|
960 |
L
|
I
|
C
|
I
|
F
|
T
|
F
|
C
|
F
|
F
|
970 |
ICL1 | TM2 | ||||||||
R
|
G
|
L
|
Q
|
S
|
D
|
R
|
N
|
T
|
I
|
980 |
H
|
K
|
N
|
L
|
C
|
I
|
S
|
L
|
F
|
V
|
990 |
A
|
E
|
L
|
L
|
F
|
L
|
I
|
G
|
I
|
N
|
1000 |
ECL1 | TM3 | ||||||||
R
|
T
|
D
|
Q
|
P
|
I
|
A
|
C
|
A
|
V
|
1010 |
F
|
A
|
A
|
L
|
L
|
H
|
F
|
F
|
F
|
L
|
1020 |
A
|
A
|
F
|
T
|
W
|
M
|
F
|
L
|
E
|
G
|
1030 |
V
|
Q
|
L
|
Y
|
I
|
M
|
L
|
V
|
E
|
V
|
1040 |
ICL2 | TM4 | ||||||||
F
|
E
|
S
|
E
|
H
|
S
|
R
|
R
|
K
|
Y
|
1050 |
F
|
Y
|
L
|
V
|
G
|
Y
|
G
|
M
|
P
|
A
|
1060 |
L
|
I
|
V
|
A
|
V
|
S
|
A
|
A
|
V
|
D
|
1070 |
ECL2 | |||||||||
Y
|
R
|
S
|
Y
|
G
|
T
|
D
|
K
|
V
|
C
|
1080 |
TM5 | |||||||||
W
|
L
|
R
|
L
|
D
|
T
|
Y
|
F
|
I
|
W
|
1090 |
S
|
F
|
I
|
G
|
P
|
A
|
T
|
L
|
I
|
I
|
1100 |
M
|
L
|
N
|
V
|
I
|
F
|
L
|
G
|
I
|
A
|
1110 |
L
|
Y
|
K
|
M
|
F
|
H
|
H
|
T
|
A
|
I
|
1120 |
ICL3 | |||||||||
L
|
K
|
P
|
E
|
S
|
G
|
C
|
L
|
D
|
N
|
1130 |
TM6 | |||||||||
I
|
N
|
Y
|
E
|
D
|
N
|
R
|
P
|
F
|
I
|
1140 |
K
|
S
|
W
|
V
|
I
|
G
|
A
|
I
|
A
|
L
|
1150 |
L
|
C
|
L
|
L
|
G
|
L
|
T
|
W
|
A
|
F
|
1160 |
ECL3 | |||||||||
G
|
L
|
M
|
Y
|
I
|
N
|
E
|
S
|
T
|
V
|
1170 |
TM7 | |||||||||
I
|
M
|
A
|
Y
|
L
|
F
|
T
|
I
|
F
|
N
|
1180 |
S
|
L
|
Q
|
G
|
M
|
F
|
I
|
F
|
I
|
F
|
1190 |
H8 | |||||||||
H
|
C
|
V
|
L
|
Q
|
K
|
K
|
V
|
R
|
K
|
1200 |
E
|
Y
|
G
|
K
|
C
|
L
|
R
|
T
|
H
|
C
|
1210 |
C-term | |||||||||
C
|
S
|
G
|
K
|
S
|
T
|
E
|
S
|
S
|
I
|
1220 |
G
|
S
|
G
|
K
|
T
|
S
|
G
|
S
|
R
|
T
|
1230 |
P
|
G
|
R
|
Y
|
S
|
T
|
G
|
S
|
Q
|
S
|
1240 |
R
|
I
|
R
|
R
|
M
|
W
|
N
|
D
|
T
|
V
|
1250 |
R
|
K
|
Q
|
S
|
E
|
S
|
S
|
F
|
I
|
T
|
1260 |
G
|
D
|
I
|
N
|
S
|
S
|
A
|
S
|
L
|
N
|
1270 |
R
|
E
|
G
|
L
|
L
|
N
|
N
|
A
|
R
|
D
|
1280 |
T
|
S
|
V
|
M
|
D
|
T
|
L
|
P
|
L
|
N
|
1290 |
G
|
N
|
H
|
G
|
N
|
S
|
Y
|
S
|
I
|
A
|
1300 |
G
|
G
|
E
|
Y
|
L
|
S
|
N
|
C
|
V
|
Q
|
1310 |
I
|
I
|
D
|
R
|
G
|
Y
|
N
|
H
|
N
|
E
|
1320 |
T
|
A
|
L
|
E
|
K
|
K
|
I
|
L
|
K
|
E
|
1330 |
L
|
T
|
S
|
N
|
Y
|
I
|
P
|
S
|
Y
|
L
|
1340 |
N
|
N
|
H
|
E
|
R
|
S
|
S
|
E
|
Q
|
N
|
1350 |
R
|
N
|
M
|
M
|
N
|
K
|
L
|
V
|
N
|
N
|
1360 |
L
|
G
|
S
|
G
|
S
|
E
|
D
|
D
|
A
|
I
|
1370 |
V
|
L
|
D
|
D
|
A
|
A
|
S
|
F
|
N
|
H
|
1380 |
E
|
E
|
S
|
L
|
G
|
L
|
E
|
L
|
I
|
H
|
1390 |
E
|
E
|
S
|
D
|
A
|
P
|
L
|
L
|
P
|
P
|
1400 |
R
|
V
|
Y
|
S
|
T
|
D
|
N
|
H
|
Q
|
P
|
1410 |
H
|
H
|
Y
|
S
|
R
|
R
|
R
|
F
|
P
|
Q
|
1420 |
D
|
H
|
S
|
E
|
S
|
F
|
F
|
P
|
L
|
L
|
1430 |
T
|
D
|
E
|
H
|
T
|
E
|
D
|
L
|
Q
|
S
|
1440 |
P
|
H
|
R
|
D
|
S
|
L
|
Y
|
T
|
S
|
M
|
1450 |
P
|
A
|
L
|
A
|
G
|
V
|
P
|
A
|
A
|
D
|
1460 |
S
|
V
|
T
|
T
|
S
|
T
|
Q
|
T
|
E
|
A
|
1470 |
A
|
A
|
A
|
K
|
G
|
G
|
D
|
A
|
E
|
D
|
1480 |
V
|
Y
|
Y
|
K
|
S
|
M
|
P
|
N
|
L
|
G
|
1490 |
S
|
R
|
N
|
H
|
V
|
H
|
P
|
L
|
H
|
A
|
1500 |
Y
|
Y
|
Q
|
L
|
G
|
R
|
G
|
S
|
S
|
D
|
1510 |
G
|
F
|
I
|
V
|
P
|
P
|
N
|
K
|
D
|
G
|
1520 |
A
|
S
|
P
|
E
|
G
|
T
|
S
|
K
|
G
|
P
|
1530 |
A
|
H
|
L
|
V
|
T
|
S
|
L
|
1537 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|