mrgrd_human
NAME
7Y15
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
mrgrd_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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N
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Q
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T
|
L
|
N
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S
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S
|
G
|
T
|
10 |
V
|
E
|
S
|
A
|
L
|
N
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Y
|
S
|
R
|
G
|
20 |
TM1 | |||||||||
S
|
T
|
V
|
H
|
T
|
A
|
Y
|
L
|
V
|
L
|
30 |
S
|
S
|
L
|
A
|
M
|
F
|
T
|
C
|
L
|
C
|
40 |
G
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M
|
A
|
G
|
N
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S
|
M
|
V
|
I
|
W
|
50 |
ICL1 | |||||||||
L
|
L
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G
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F
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R
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M
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H
|
R
|
N
|
P
|
60 |
TM2 | |||||||||
F
|
C
|
I
|
Y
|
I
|
L
|
N
|
L
|
A
|
A
|
70 |
A
|
D
|
L
|
L
|
F
|
L
|
F
|
S
|
M
|
A
|
80 |
S
|
T
|
L
|
S
|
L
|
E
|
T
|
Q
|
P
|
L
|
90 |
ECL1 | TM3 | ||||||||
V
|
N
|
T
|
T
|
D
|
K
|
V
|
H
|
E
|
L
|
100 |
M
|
K
|
R
|
L
|
M
|
Y
|
F
|
A
|
Y
|
T
|
110 |
V
|
G
|
L
|
S
|
L
|
L
|
T
|
A
|
I
|
S
|
120 |
T
|
Q
|
R
|
C
|
L
|
S
|
V
|
L
|
F
|
P
|
130 |
ICL2 | TM4 | ||||||||
I
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W
|
F
|
K
|
C
|
H
|
R
|
P
|
R
|
H
|
140 |
L
|
S
|
A
|
W
|
V
|
C
|
G
|
L
|
L
|
W
|
150 |
T
|
L
|
C
|
L
|
L
|
M
|
N
|
G
|
L
|
T
|
160 |
ECL2 | |||||||||
S
|
S
|
F
|
C
|
S
|
K
|
F
|
L
|
K
|
F
|
170 |
TM5 | |||||||||
N
|
E
|
D
|
R
|
C
|
F
|
R
|
V
|
D
|
M
|
180 |
V
|
Q
|
A
|
A
|
L
|
I
|
M
|
G
|
V
|
L
|
190 |
T
|
P
|
V
|
M
|
T
|
L
|
S
|
S
|
L
|
T
|
200 |
L
|
F
|
V
|
W
|
V
|
R
|
R
|
S
|
S
|
Q
|
210 |
ICL3 | TM6 | ||||||||
Q
|
W
|
R
|
R
|
Q
|
P
|
T
|
R
|
L
|
F
|
220 |
V
|
V
|
V
|
L
|
A
|
S
|
V
|
L
|
V
|
F
|
230 |
L
|
I
|
C
|
S
|
L
|
P
|
L
|
S
|
I
|
Y
|
240 |
W
|
F
|
V
|
L
|
Y
|
W
|
L
|
S
|
L
|
P
|
250 |
ECL3 | TM7 | ||||||||
P
|
E
|
M
|
Q
|
V
|
L
|
C
|
F
|
S
|
L
|
260 |
S
|
R
|
L
|
S
|
S
|
S
|
V
|
S
|
S
|
S
|
270 |
A
|
N
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P
|
V
|
I
|
Y
|
F
|
L
|
V
|
G
|
280 |
H8 | |||||||||
S
|
R
|
R
|
S
|
H
|
R
|
L
|
P
|
T
|
R
|
290 |
S
|
L
|
G
|
T
|
V
|
L
|
Q
|
Q
|
A
|
L
|
300 |
C-term | |||||||||
R
|
E
|
E
|
P
|
E
|
L
|
E
|
G
|
G
|
E
|
310 |
T
|
P
|
T
|
V
|
G
|
T
|
N
|
E
|
M
|
G
|
320 |
A
|
|
|||||||||
N-term | |||||||||
|
L
|
N
|
S
|
S
|
G
|
T
|
V
|
E
|
S
|
13 |
A
|
L
|
N
|
Y
|
S
|
R
|
G
|
S
|
T
|
V
|
23 |
TM1 | |||||||||
H
|
T
|
A
|
Y
|
L
|
V
|
L
|
S
|
S
|
L
|
33 |
A
|
M
|
F
|
T
|
C
|
L
|
C
|
G
|
M
|
A
|
43 |
G
|
N
|
S
|
M
|
V
|
I
|
W
|
L
|
L
|
G
|
53 |
ICL1 | TM2 | ||||||||
F
|
R
|
M
|
H
|
R
|
N
|
P
|
F
|
C
|
I
|
63 |
Y
|
I
|
L
|
N
|
L
|
A
|
A
|
A
|
D
|
L
|
73 |
L
|
F
|
L
|
F
|
S
|
M
|
A
|
S
|
T
|
L
|
83 |
ECL1 | |||||||||
S
|
L
|
E
|
T
|
Q
|
P
|
L
|
V
|
N
|
T
|
93 |
TM3 | |||||||||
T
|
D
|
K
|
V
|
H
|
E
|
L
|
M
|
K
|
R
|
103 |
L
|
M
|
Y
|
F
|
A
|
Y
|
T
|
V
|
G
|
L
|
113 |
S
|
L
|
L
|
T
|
A
|
I
|
S
|
T
|
Q
|
R
|
123 |
ICL2 | |||||||||
C
|
L
|
S
|
V
|
L
|
F
|
P
|
I
|
W
|
F
|
133 |
TM4 | |||||||||
K
|
C
|
H
|
R
|
P
|
R
|
H
|
L
|
S
|
A
|
143 |
W
|
V
|
C
|
G
|
L
|
L
|
W
|
T
|
L
|
C
|
153 |
L
|
L
|
M
|
N
|
G
|
L
|
T
|
S
|
S
|
F
|
163 |
ECL2 | |||||||||
C
|
S
|
K
|
F
|
L
|
K
|
F
|
N
|
E
|
D
|
173 |
TM5 | |||||||||
R
|
C
|
F
|
R
|
V
|
D
|
M
|
V
|
Q
|
A
|
183 |
A
|
L
|
I
|
M
|
G
|
V
|
L
|
T
|
P
|
V
|
193 |
M
|
T
|
L
|
S
|
S
|
L
|
T
|
L
|
F
|
V
|
203 |
ICL3 | |||||||||
W
|
V
|
R
|
R
|
S
|
S
|
Q
|
Q
|
W
|
R
|
213 |
TM6 | |||||||||
R
|
Q
|
P
|
T
|
R
|
L
|
F
|
V
|
V
|
V
|
223 |
L
|
A
|
S
|
V
|
L
|
V
|
F
|
L
|
I
|
C
|
233 |
S
|
L
|
P
|
L
|
S
|
I
|
Y
|
W
|
F
|
V
|
243 |
ECL3 | |||||||||
L
|
Y
|
W
|
L
|
S
|
L
|
P
|
P
|
E
|
M
|
253 |
TM7 | |||||||||
Q
|
V
|
L
|
C
|
F
|
S
|
L
|
S
|
R
|
L
|
263 |
S
|
S
|
S
|
V
|
S
|
S
|
S
|
A
|
N
|
P
|
273 |
H8 | |||||||||
V
|
I
|
Y
|
F
|
L
|
V
|
G
|
S
|
R
|
R
|
283 |
S
|
H
|
R
|
L
|
P
|
T
|
R
|
S
|
L
|
G
|
293 |
C-term | |||||||||
T
|
V
|
L
|
Q
|
Q
|
A
|
L
|
R
|
E
|
E
|
303 |
P
|
E
|
L
|
E
|
G
|
G
|
E
|
T
|
P
|
T
|
313 |
V
|
G
|
T
|
N
|
E
|
M
|
G
|
A
|
321 |
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
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DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
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Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
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