5ht1a_human
NAME
8FYX
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
5ht1a_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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D
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V
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L
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S
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P
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G
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Q
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G
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N
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10 |
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|
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N
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T
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T
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S
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P
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P
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A
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P
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F
|
E
|
20 |
|
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|
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|||||
T
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G
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G
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N
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T
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T
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G
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I
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S
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D
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30 |
TM1 | |||||||||
V
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T
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V
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S
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Y
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Q
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V
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I
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T
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S
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40 |
L
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L
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L
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G
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T
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L
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I
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F
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C
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A
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50 |
V
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L
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G
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N
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A
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C
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V
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V
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A
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A
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60 |
ICL1 | |||||||||
I
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A
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L
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E
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R
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S
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L
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Q
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N
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V
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70 |
TM2 | |||||||||
A
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N
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Y
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L
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I
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G
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S
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L
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A
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V
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80 |
T
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D
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L
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M
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V
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S
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V
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L
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V
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L
|
90 |
P
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M
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A
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A
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L
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Y
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Q
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V
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L
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N
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100 |
ECL1 | TM3 | ||||||||
K
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W
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T
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L
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G
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Q
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V
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T
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C
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D
|
110 |
L
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F
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I
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A
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L
|
D
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V
|
L
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C
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C
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120 |
T
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S
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S
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I
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L
|
H
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L
|
C
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A
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I
|
130 |
A
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L
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D
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R
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Y
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W
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A
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I
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T
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D
|
140 |
ICL2 | |||||||||
P
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I
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D
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Y
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V
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N
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K
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R
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T
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P
|
150 |
TM4 | |||||||||
R
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R
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A
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A
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A
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L
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I
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S
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L
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T
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160 |
W
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L
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I
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G
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F
|
L
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I
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S
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I
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P
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170 |
ECL2 | |||||||||
P
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M
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L
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G
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W
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R
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T
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P
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E
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D
|
180 |
R
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S
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D
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P
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D
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A
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C
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T
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I
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S
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190 |
TM5 | |||||||||
K
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D
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H
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G
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Y
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T
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I
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Y
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S
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T
|
200 |
F
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G
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A
|
F
|
Y
|
I
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P
|
L
|
L
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L
|
210 |
M
|
L
|
V
|
L
|
Y
|
G
|
R
|
I
|
F
|
R
|
220 |
A
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A
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R
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F
|
R
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I
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R
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K
|
T
|
V
|
230 |
ICL3 | |||||||||
K
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K
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V
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E
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K
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T
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G
|
A
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D
|
T
|
240 |
R
|
H
|
G
|
A
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S
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P
|
A
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P
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Q
|
P
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250 |
K
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K
|
S
|
V
|
N
|
G
|
E
|
S
|
G
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S
|
260 |
R
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N
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W
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R
|
L
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G
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V
|
E
|
S
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K
|
270 |
A
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G
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G
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A
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L
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C
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A
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N
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G
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A
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280 |
V
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R
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Q
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G
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D
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D
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G
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A
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A
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L
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290 |
E
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V
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I
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E
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V
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H
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R
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V
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G
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N
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300 |
S
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K
|
E
|
H
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L
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P
|
L
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P
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S
|
E
|
310 |
A
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G
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P
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T
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P
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C
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A
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P
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A
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S
|
320 |
TM6 | |||||||||
F
|
E
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R
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K
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N
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E
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R
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N
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A
|
E
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330 |
A
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K
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R
|
K
|
M
|
A
|
L
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A
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R
|
E
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340 |
R
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K
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T
|
V
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K
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T
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L
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G
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I
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I
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350 |
M
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G
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T
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F
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I
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L
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C
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W
|
L
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P
|
360 |
F
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F
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I
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V
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A
|
L
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V
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L
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P
|
F
|
370 |
ECL3 | TM7 | ||||||||
C
|
E
|
S
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S
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C
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H
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M
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P
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T
|
L
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380 |
L
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G
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A
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I
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I
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N
|
W
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L
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G
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Y
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390 |
S
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N
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S
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L
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L
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N
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P
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V
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I
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Y
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400 |
H8 | |||||||||
A
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Y
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F
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N
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K
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D
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F
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Q
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N
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A
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410 |
F
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K
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K
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I
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I
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K
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C
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K
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F
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C
|
420 |
C-term | |||||
R
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Q
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||||
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|||||||||
N-term | TM1 | ||||||||
|
T
|
G
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I
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S
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D
|
V
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T
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V
|
S
|
34 |
Y
|
Q
|
V
|
I
|
T
|
S
|
L
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L
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L
|
G
|
44 |
T
|
L
|
I
|
F
|
C
|
A
|
V
|
L
|
G
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N
|
54 |
A
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C
|
V
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V
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A
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A
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I
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A
|
L
|
E
|
64 |
ICL1 | TM2 | ||||||||
R
|
S
|
L
|
Q
|
N
|
V
|
A
|
N
|
Y
|
L
|
74 |
I
|
G
|
S
|
L
|
A
|
V
|
T
|
D
|
L
|
M
|
84 |
V
|
S
|
V
|
L
|
V
|
L
|
P
|
M
|
A
|
A
|
94 |
ECL1 | |||||||||
L
|
Y
|
Q
|
V
|
L
|
N
|
K
|
W
|
T
|
L
|
104 |
TM3 | |||||||||
G
|
Q
|
V
|
T
|
C
|
D
|
L
|
F
|
I
|
A
|
114 |
L
|
D
|
V
|
L
|
C
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C
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T
|
S
|
S
|
I
|
124 |
L
|
H
|
L
|
C
|
A
|
I
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A
|
L
|
D
|
R
|
134 |
ICL2 | |||||||||
Y
|
W
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A
|
I
|
T
|
D
|
P
|
I
|
D
|
Y
|
144 |
TM4 | |||||||||
V
|
N
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K
|
R
|
T
|
P
|
R
|
R
|
A
|
A
|
154 |
A
|
L
|
I
|
S
|
L
|
T
|
W
|
L
|
I
|
G
|
164 |
F
|
L
|
I
|
S
|
I
|
P
|
P
|
M
|
L
|
G
|
174 |
ECL2 | |||||||||
W
|
R
|
T
|
P
|
E
|
D
|
R
|
S
|
D
|
P
|
184 |
TM5 | |||||||||
D
|
A
|
C
|
T
|
I
|
S
|
K
|
D
|
H
|
G
|
194 |
Y
|
T
|
I
|
Y
|
S
|
T
|
F
|
G
|
A
|
F
|
204 |
Y
|
I
|
P
|
L
|
L
|
L
|
M
|
L
|
V
|
L
|
214 |
Y
|
G
|
R
|
I
|
F
|
R
|
A
|
A
|
R
|
F
|
224 |
R
|
I
|
R
|
K
|
T
|
V
|
K
|
K
|
V
|
E
|
234 |
ICL3 | |||||||||
K
|
T
|
G
|
A
|
D
|
T
|
R
|
H
|
G
|
A
|
244 |
S
|
P
|
A
|
P
|
Q
|
P
|
K
|
K
|
S
|
V
|
254 |
N
|
G
|
E
|
S
|
G
|
S
|
R
|
N
|
W
|
R
|
264 |
L
|
G
|
V
|
E
|
S
|
K
|
A
|
G
|
G
|
A
|
274 |
L
|
C
|
A
|
N
|
G
|
A
|
V
|
R
|
Q
|
G
|
284 |
D
|
D
|
G
|
A
|
A
|
L
|
E
|
V
|
I
|
E
|
294 |
V
|
H
|
R
|
V
|
G
|
N
|
S
|
K
|
E
|
H
|
304 |
L
|
P
|
L
|
P
|
S
|
E
|
A
|
G
|
P
|
T
|
314 |
P
|
C
|
A
|
P
|
A
|
S
|
F
|
E
|
R
|
K
|
324 |
TM6 | |||||||||
N
|
E
|
R
|
N
|
A
|
E
|
A
|
K
|
R
|
K
|
334 |
M
|
A
|
L
|
A
|
R
|
E
|
R
|
K
|
T
|
V
|
344 |
K
|
T
|
L
|
G
|
I
|
I
|
M
|
G
|
T
|
F
|
354 |
I
|
L
|
C
|
W
|
L
|
P
|
F
|
F
|
I
|
V
|
364 |
A
|
L
|
V
|
L
|
P
|
F
|
C
|
E
|
S
|
S
|
374 |
ECL3 | TM7 | ||||||||
C
|
H
|
M
|
P
|
T
|
L
|
L
|
G
|
A
|
I
|
384 |
I
|
N
|
W
|
L
|
G
|
Y
|
S
|
N
|
S
|
L
|
394 |
L
|
N
|
P
|
V
|
I
|
Y
|
A
|
Y
|
F
|
N
|
404 |
H8 | |||||||||
K
|
D
|
F
|
Q
|
N
|
A
|
F
|
K
|
K
|
I
|
414 |
C-term | |||||||
I
|
K
|
C
|
K
|
F
|
C
|
R
|
Q
|
422 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|