crfr1_human
NAME
8GTI
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
crfr1_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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G
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G
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H
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P
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Q
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L
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R
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L
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V
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10 |
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|
|
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K
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A
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L
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L
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L
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L
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G
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L
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N
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P
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20 |
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|
|
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V
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S
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A
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S
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L
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Q
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D
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Q
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H
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C
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30 |
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|
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|
|
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E
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S
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L
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S
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L
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A
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S
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N
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I
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S
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40 |
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|
|
|
|
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G
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L
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Q
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C
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N
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A
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S
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V
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D
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L
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50 |
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|
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|
|
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I
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G
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T
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C
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W
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P
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R
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S
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P
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A
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60 |
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|
|
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G
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Q
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L
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V
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V
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R
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P
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C
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P
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A
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70 |
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|
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F
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F
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Y
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G
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V
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R
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Y
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N
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T
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T
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80 |
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N
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N
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G
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Y
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R
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E
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C
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L
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A
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N
|
90 |
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|
|
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G
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S
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W
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A
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A
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R
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V
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N
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Y
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S
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100 |
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|||||||
TM1 | |||||||||
E
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C
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Q
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E
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I
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L
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N
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E
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E
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K
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110 |
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|||||||||
K
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S
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K
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V
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H
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Y
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H
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V
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A
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V
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120 |
I
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I
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N
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Y
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L
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G
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H
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C
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I
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S
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130 |
L
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V
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A
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L
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L
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V
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A
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F
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V
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L
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140 |
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|||||||||
ICL1 | |||||||||
F
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L
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R
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L
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R
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S
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I
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R
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C
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L
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150 |
TM2 | |||||||||
R
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N
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I
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I
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H
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W
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N
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L
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I
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S
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160 |
A
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F
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I
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L
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R
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N
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A
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T
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W
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F
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170 |
ECL1 | |||||||||
V
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V
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Q
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L
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T
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M
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S
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P
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E
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V
|
180 |
TM3 | |||||||||
H
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Q
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S
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N
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V
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G
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W
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C
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R
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L
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190 |
V
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T
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A
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A
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Y
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N
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Y
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F
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H
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V
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200 |
T
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N
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F
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F
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W
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M
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F
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G
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E
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G
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210 |
C
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Y
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L
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H
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T
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A
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I
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V
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L
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T
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220 |
ICL2 | TM4 | ||||||||
Y
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S
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T
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D
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R
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L
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R
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K
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W
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M
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230 |
F
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I
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C
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I
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G
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W
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G
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V
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P
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F
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240 |
P
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I
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I
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V
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A
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W
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A
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I
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G
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K
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250 |
ECL2 | |||||||||
L
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Y
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Y
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D
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N
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E
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K
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C
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W
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F
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260 |
TM5 | |||||||||
G
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K
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R
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P
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G
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V
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Y
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T
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D
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Y
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270 |
I
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Y
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Q
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G
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P
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M
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I
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L
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V
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L
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280 |
L
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I
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N
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F
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I
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F
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L
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F
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N
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I
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290 |
V
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R
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I
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L
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M
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T
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K
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L
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R
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A
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300 |
ICL3 | TM6 | ||||||||
S
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T
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T
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S
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E
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T
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I
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Q
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Y
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R
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310 |
K
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A
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V
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K
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A
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T
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L
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V
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L
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L
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320 |
P
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L
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L
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G
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I
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T
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Y
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M
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L
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F
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330 |
ECL3 | TM7 | ||||||||
F
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V
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N
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P
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G
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E
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D
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E
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V
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S
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340 |
R
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V
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V
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F
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I
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Y
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F
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N
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S
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F
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350 |
L
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E
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S
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F
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Q
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G
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F
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F
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V
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S
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360 |
H8 | |||||||||
V
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F
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Y
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C
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F
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L
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N
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S
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E
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V
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370 |
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|||
R
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S
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A
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I
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R
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K
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R
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W
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H
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R
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380 |
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|
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W
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Q
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D
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K
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H
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S
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I
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R
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A
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R
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390 |
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C-term | |||||||||
V
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A
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R
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A
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M
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S
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I
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P
|
T
|
S
|
400 |
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|
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P
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T
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R
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V
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S
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F
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H
|
S
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I
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K
|
410 |
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|
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Q
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S
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T
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A
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V
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|||||||||
TM1 | |||||||||
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E
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I
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L
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N
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E
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E
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K
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K
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S
|
112 |
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|||||||||
K
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V
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H
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Y
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H
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V
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A
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A
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I
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I
|
122 |
N
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Y
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L
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G
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H
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C
|
I
|
S
|
L
|
V
|
132 |
A
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L
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L
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V
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A
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F
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V
|
L
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F
|
L
|
142 |
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|||||||||
ICL1 | TM2 | ||||||||
R
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A
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R
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S
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I
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R
|
C
|
L
|
R
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N
|
152 |
I
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I
|
H
|
W
|
N
|
L
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I
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S
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A
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F
|
162 |
I
|
L
|
R
|
N
|
A
|
T
|
W
|
F
|
V
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V
|
172 |
ECL1 | |||||||||
Q
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L
|
T
|
M
|
S
|
P
|
E
|
V
|
H
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Q
|
182 |
TM3 | |||||||||
S
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N
|
V
|
G
|
W
|
C
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R
|
L
|
V
|
T
|
192 |
A
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A
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Y
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N
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Y
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F
|
H
|
V
|
T
|
N
|
202 |
F
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F
|
W
|
M
|
F
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G
|
E
|
G
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C
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Y
|
212 |
L
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H
|
T
|
A
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I
|
V
|
L
|
T
|
Y
|
S
|
222 |
ICL2 | TM4 | ||||||||
T
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D
|
R
|
L
|
R
|
K
|
W
|
M
|
F
|
I
|
232 |
C
|
I
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G
|
W
|
G
|
V
|
P
|
F
|
P
|
I
|
242 |
I
|
V
|
A
|
W
|
A
|
I
|
G
|
K
|
L
|
Y
|
252 |
ECL2 | |||||||||
Y
|
D
|
N
|
E
|
K
|
C
|
W
|
F
|
G
|
K
|
262 |
TM5 | |||||||||
R
|
P
|
G
|
V
|
Y
|
T
|
D
|
Y
|
I
|
Y
|
272 |
Q
|
G
|
P
|
M
|
I
|
L
|
V
|
L
|
L
|
I
|
282 |
N
|
F
|
I
|
F
|
L
|
F
|
N
|
I
|
V
|
R
|
292 |
ICL3 | |||||||||
I
|
L
|
M
|
T
|
K
|
L
|
R
|
A
|
S
|
T
|
302 |
TM6 | |||||||||
T
|
S
|
E
|
T
|
I
|
Q
|
Y
|
R
|
K
|
A
|
312 |
V
|
K
|
A
|
T
|
L
|
V
|
L
|
L
|
P
|
L
|
322 |
L
|
G
|
I
|
T
|
Y
|
M
|
L
|
F
|
F
|
V
|
332 |
ECL3 | TM7 | ||||||||
N
|
P
|
G
|
E
|
D
|
E
|
V
|
S
|
R
|
V
|
342 |
V
|
F
|
I
|
Y
|
F
|
N
|
S
|
F
|
L
|
E
|
352 |
S
|
F
|
Q
|
G
|
F
|
F
|
V
|
S
|
V
|
F
|
362 |
H8 | |||||||||
Y
|
C
|
F
|
L
|
N
|
S
|
E
|
V
|
R
|
S
|
372 |
|
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A
|
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SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
120 | | V | A |
144 | | L | A |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
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Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|