hrh4_human
NAME
8HN8
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
hrh4_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
|
|
|
|||||||
N-term | |||||||||
M
|
P
|
D
|
T
|
N
|
S
|
T
|
I
|
N
|
L
|
10 |
TM1 | |||||||||
S
|
L
|
S
|
T
|
R
|
V
|
T
|
L
|
A
|
F
|
20 |
F
|
M
|
S
|
L
|
V
|
A
|
F
|
A
|
I
|
M
|
30 |
L
|
G
|
N
|
A
|
L
|
V
|
I
|
L
|
A
|
F
|
40 |
ICL1 | TM2 | ||||||||
V
|
V
|
D
|
K
|
N
|
L
|
R
|
H
|
R
|
S
|
50 |
S
|
Y
|
F
|
F
|
L
|
N
|
L
|
A
|
I
|
S
|
60 |
D
|
F
|
F
|
V
|
G
|
V
|
I
|
S
|
I
|
P
|
70 |
L
|
Y
|
I
|
P
|
H
|
T
|
L
|
F
|
E
|
W
|
80 |
ECL1 | TM3 | ||||||||
D
|
F
|
G
|
K
|
E
|
I
|
C
|
V
|
F
|
W
|
90 |
L
|
T
|
T
|
D
|
Y
|
L
|
L
|
C
|
T
|
A
|
100 |
S
|
V
|
Y
|
N
|
I
|
V
|
L
|
I
|
S
|
Y
|
110 |
D
|
R
|
Y
|
L
|
S
|
V
|
S
|
N
|
A
|
V
|
120 |
ICL2 | TM4 | ||||||||
S
|
Y
|
R
|
T
|
Q
|
H
|
T
|
G
|
V
|
L
|
130 |
K
|
I
|
V
|
T
|
L
|
M
|
V
|
A
|
V
|
W
|
140 |
V
|
L
|
A
|
F
|
L
|
V
|
N
|
G
|
P
|
M
|
150 |
I
|
L
|
V
|
S
|
E
|
S
|
W
|
K
|
D
|
E
|
160 |
ECL2 | |||||||||
G
|
S
|
E
|
C
|
E
|
P
|
G
|
F
|
F
|
S
|
170 |
TM5 | |||||||||
E
|
W
|
Y
|
I
|
L
|
A
|
I
|
T
|
S
|
F
|
180 |
L
|
E
|
F
|
V
|
I
|
P
|
V
|
I
|
L
|
V
|
190 |
A
|
Y
|
F
|
N
|
M
|
N
|
I
|
Y
|
W
|
S
|
200 |
L
|
W
|
K
|
R
|
D
|
H
|
L
|
S
|
R
|
C
|
210 |
ICL3 | |||||||||
Q
|
S
|
H
|
P
|
G
|
L
|
T
|
A
|
V
|
S
|
220 |
S
|
N
|
I
|
C
|
G
|
H
|
S
|
F
|
R
|
G
|
230 |
R
|
L
|
S
|
S
|
R
|
R
|
S
|
L
|
S
|
A
|
240 |
S
|
T
|
E
|
V
|
P
|
A
|
S
|
F
|
H
|
S
|
250 |
E
|
R
|
Q
|
R
|
R
|
K
|
S
|
S
|
L
|
M
|
260 |
F
|
S
|
S
|
R
|
T
|
K
|
M
|
N
|
S
|
N
|
270 |
T
|
I
|
A
|
S
|
K
|
M
|
G
|
S
|
F
|
S
|
280 |
Q
|
S
|
D
|
S
|
V
|
A
|
L
|
H
|
Q
|
R
|
290 |
TM6 | |||||||||
E
|
H
|
V
|
E
|
L
|
L
|
R
|
A
|
R
|
R
|
300 |
L
|
A
|
K
|
S
|
L
|
A
|
I
|
L
|
L
|
G
|
310 |
V
|
F
|
A
|
V
|
C
|
W
|
A
|
P
|
Y
|
S
|
320 |
L
|
F
|
T
|
I
|
V
|
L
|
S
|
F
|
Y
|
S
|
330 |
ECL3 | TM7 | ||||||||
S
|
A
|
T
|
G
|
P
|
K
|
S
|
V
|
W
|
Y
|
340 |
R
|
I
|
A
|
F
|
W
|
L
|
Q
|
W
|
F
|
N
|
350 |
S
|
F
|
V
|
N
|
P
|
L
|
L
|
Y
|
P
|
L
|
360 |
H8 | |||||||||
C
|
H
|
K
|
R
|
F
|
Q
|
K
|
A
|
F
|
L
|
370 |
K
|
I
|
F
|
C
|
I
|
K
|
K
|
Q
|
P
|
L
|
380 |
C-term | |||||||||
P
|
S
|
Q
|
H
|
S
|
R
|
S
|
V
|
S
|
S
|
390 |
|
|||||||||
N-term | |||||||||
|
T
|
N
|
S
|
T
|
I
|
N
|
L
|
S
|
L
|
12 |
TM1 | |||||||||
S
|
T
|
R
|
V
|
T
|
L
|
A
|
F
|
F
|
M
|
22 |
S
|
L
|
V
|
A
|
F
|
A
|
I
|
M
|
L
|
G
|
32 |
N
|
A
|
L
|
V
|
I
|
L
|
A
|
F
|
V
|
V
|
42 |
ICL1 | TM2 | ||||||||
D
|
K
|
N
|
L
|
R
|
H
|
R
|
S
|
S
|
Y
|
52 |
F
|
F
|
L
|
N
|
L
|
A
|
I
|
S
|
D
|
F
|
62 |
F
|
V
|
G
|
V
|
I
|
S
|
I
|
P
|
L
|
Y
|
72 |
ECL1 | |||||||||
I
|
P
|
H
|
T
|
L
|
F
|
E
|
W
|
D
|
F
|
82 |
TM3 | |||||||||
G
|
K
|
E
|
I
|
C
|
V
|
F
|
W
|
L
|
T
|
92 |
T
|
D
|
Y
|
L
|
L
|
C
|
T
|
A
|
S
|
V
|
102 |
Y
|
N
|
I
|
V
|
L
|
I
|
S
|
Y
|
D
|
R
|
112 |
ICL2 | |||||||||
Y
|
L
|
S
|
V
|
S
|
N
|
A
|
V
|
S
|
Y
|
122 |
TM4 | |||||||||
R
|
T
|
Q
|
H
|
T
|
G
|
V
|
L
|
K
|
I
|
132 |
V
|
T
|
L
|
M
|
V
|
A
|
V
|
W
|
V
|
L
|
142 |
A
|
F
|
L
|
V
|
N
|
G
|
P
|
M
|
I
|
L
|
152 |
ECL2 | |||||||||
V
|
S
|
E
|
S
|
W
|
K
|
D
|
E
|
G
|
S
|
162 |
E
|
C
|
E
|
P
|
G
|
F
|
F
|
S
|
E
|
W
|
172 |
TM5 | |||||||||
Y
|
I
|
L
|
A
|
I
|
T
|
S
|
F
|
L
|
E
|
182 |
F
|
V
|
I
|
P
|
V
|
I
|
L
|
V
|
A
|
Y
|
192 |
F
|
N
|
M
|
N
|
I
|
Y
|
W
|
S
|
L
|
W
|
202 |
K
|
R
|
D
|
H
|
L
|
S
|
R
|
C
|
Q
|
S
|
212 |
ICL3 | |||||||||
H
|
P
|
G
|
L
|
T
|
A
|
V
|
S
|
S
|
N
|
222 |
I
|
C
|
G
|
H
|
S
|
F
|
R
|
G
|
R
|
L
|
232 |
S
|
S
|
R
|
R
|
S
|
L
|
S
|
A
|
S
|
T
|
242 |
E
|
V
|
P
|
A
|
S
|
F
|
H
|
S
|
E
|
R
|
252 |
Q
|
R
|
R
|
K
|
S
|
S
|
L
|
M
|
F
|
S
|
262 |
S
|
R
|
T
|
K
|
M
|
N
|
S
|
N
|
T
|
I
|
272 |
A
|
S
|
K
|
M
|
G
|
S
|
F
|
S
|
Q
|
S
|
282 |
D
|
S
|
V
|
A
|
L
|
H
|
Q
|
R
|
E
|
H
|
292 |
TM6 | |||||||||
V
|
E
|
L
|
L
|
R
|
A
|
R
|
R
|
L
|
A
|
302 |
K
|
S
|
L
|
A
|
I
|
L
|
L
|
G
|
V
|
F
|
312 |
A
|
V
|
C
|
W
|
A
|
P
|
Y
|
S
|
L
|
F
|
322 |
T
|
I
|
V
|
L
|
S
|
F
|
Y
|
S
|
S
|
A
|
332 |
ECL3 | TM7 | ||||||||
T
|
G
|
P
|
K
|
S
|
V
|
W
|
Y
|
R
|
I
|
342 |
A
|
F
|
W
|
L
|
Q
|
W
|
F
|
N
|
S
|
F
|
352 |
V
|
N
|
P
|
L
|
L
|
Y
|
P
|
L
|
C
|
H
|
362 |
H8 | |||||||||
K
|
R
|
F
|
Q
|
K
|
A
|
F
|
L
|
K
|
I
|
372 |
C-term | |||||||||
F
|
C
|
I
|
K
|
K
|
Q
|
P
|
L
|
P
|
S
|
382 |
Q
|
H
|
S
|
R
|
S
|
V
|
S
|
S
|
390 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|