ffar4_human
NAME
8T3Q
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
ffar4_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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S
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P
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E
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C
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A
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R
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A
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A
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G
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10 |
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|
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D
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A
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P
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L
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R
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S
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L
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E
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Q
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A
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20 |
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|||||||||
N
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R
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T
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R
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F
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P
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F
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F
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S
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D
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30 |
TM1 | |||||||||
V
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K
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G
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D
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H
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R
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L
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V
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L
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A
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40 |
A
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V
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E
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T
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T
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V
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L
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V
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L
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I
|
50 |
F
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A
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V
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S
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L
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L
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G
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N
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V
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C
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60 |
A
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L
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V
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L
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V
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A
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R
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R
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R
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R
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70 |
ICL1 | TM2 | ||||||||
R
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G
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A
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T
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A
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C
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L
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V
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L
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N
|
80 |
L
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F
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C
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A
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D
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L
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L
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F
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I
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S
|
90 |
A
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I
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P
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L
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V
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L
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A
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V
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R
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W
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100 |
ECL1 | TM3 | ||||||||
T
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E
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A
|
W
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L
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L
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G
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P
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V
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A
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110 |
C
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H
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L
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L
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F
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Y
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V
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M
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T
|
L
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120 |
S
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G
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S
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V
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T
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I
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L
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T
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L
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A
|
130 |
A
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V
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S
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L
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E
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R
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M
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V
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C
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I
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140 |
ICL2 | |||||||||
V
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H
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L
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Q
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R
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G
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V
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R
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G
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P
|
150 |
TM4 | |||||||||
G
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R
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R
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A
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R
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A
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V
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L
|
L
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A
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160 |
L
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I
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W
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G
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Y
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S
|
A
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V
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A
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A
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170 |
L
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P
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L
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C
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V
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F
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F
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R
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V
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V
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180 |
ECL2 | |||||||||
P
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Q
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R
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L
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P
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G
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A
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D
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Q
|
E
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190 |
I
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S
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I
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C
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T
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L
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I
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W
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P
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T
|
200 |
TM5 | |||||||||
I
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P
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G
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E
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I
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S
|
W
|
D
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V
|
S
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210 |
F
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V
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T
|
L
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N
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F
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L
|
V
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P
|
G
|
220 |
L
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V
|
I
|
V
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I
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S
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Y
|
S
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K
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I
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230 |
L
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Q
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I
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T
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K
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A
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S
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R
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K
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R
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240 |
ICL3 | TM6 | ||||||||||
L
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T
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V
|
S
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L
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A
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Y
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S
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E
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S
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||
250 |
H
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Q
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I
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R
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V
|
S
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Q
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Q
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D
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F
|
260 |
R
|
L
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F
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R
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T
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L
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F
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L
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L
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M
|
270 |
V
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S
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F
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F
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I
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M
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W
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S
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P
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I
|
280 |
I
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I
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T
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I
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L
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L
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I
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L
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I
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Q
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290 |
ECL3 | |||||||||
N
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F
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K
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Q
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D
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L
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V
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I
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W
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P
|
300 |
TM7 | |||||||||
S
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L
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F
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F
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W
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V
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V
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A
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F
|
T
|
310 |
F
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A
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N
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S
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A
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L
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N
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P
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I
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L
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320 |
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|||||||||
H8 | |||||||||
Y
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N
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M
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T
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L
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C
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R
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N
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E
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W
|
330 |
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|
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K
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K
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I
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F
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C
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C
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F
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W
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F
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P
|
340 |
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C-term | |||||||||
E
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K
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G
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A
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I
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L
|
T
|
D
|
T
|
S
|
350 |
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|
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V
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K
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R
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N
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D
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L
|
S
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I
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I
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S
|
360 |
|
G
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|||||||||
N-term | |||||||||
|
R
|
T
|
R
|
F
|
P
|
F
|
F
|
S
|
D
|
30 |
TM1 | |||||||||
V
|
K
|
G
|
D
|
H
|
R
|
L
|
V
|
L
|
A
|
40 |
A
|
V
|
E
|
T
|
T
|
V
|
L
|
V
|
L
|
I
|
50 |
F
|
A
|
V
|
S
|
L
|
L
|
G
|
N
|
V
|
C
|
60 |
A
|
L
|
V
|
L
|
V
|
A
|
R
|
R
|
R
|
R
|
70 |
ICL1 | TM2 | ||||||||
R
|
G
|
A
|
T
|
A
|
C
|
L
|
V
|
L
|
N
|
80 |
L
|
F
|
C
|
A
|
D
|
L
|
L
|
F
|
I
|
S
|
90 |
A
|
I
|
P
|
L
|
V
|
L
|
A
|
V
|
R
|
W
|
100 |
ECL1 | TM3 | ||||||||
T
|
E
|
A
|
W
|
L
|
L
|
G
|
P
|
V
|
A
|
110 |
C
|
H
|
L
|
L
|
F
|
Y
|
V
|
M
|
T
|
L
|
120 |
S
|
G
|
S
|
V
|
T
|
I
|
L
|
T
|
L
|
A
|
130 |
A
|
V
|
S
|
L
|
E
|
R
|
M
|
V
|
C
|
I
|
140 |
ICL2 | |||||||||
V
|
H
|
L
|
Q
|
R
|
G
|
V
|
R
|
G
|
P
|
150 |
TM4 | |||||||||
G
|
R
|
R
|
A
|
R
|
A
|
V
|
L
|
L
|
A
|
160 |
L
|
I
|
W
|
G
|
Y
|
S
|
A
|
V
|
A
|
A
|
170 |
L
|
P
|
L
|
C
|
V
|
F
|
F
|
R
|
V
|
V
|
180 |
ECL2 | |||||||||
P
|
Q
|
R
|
L
|
P
|
G
|
A
|
D
|
Q
|
E
|
190 |
I
|
S
|
I
|
C
|
T
|
L
|
I
|
W
|
P
|
T
|
200 |
TM5 | |||||||||
I
|
P
|
G
|
E
|
I
|
S
|
W
|
D
|
V
|
S
|
210 |
F
|
V
|
T
|
L
|
N
|
F
|
L
|
V
|
P
|
G
|
220 |
L
|
V
|
I
|
V
|
I
|
S
|
Y
|
S
|
K
|
I
|
230 |
L
|
Q
|
I
|
T
|
K
|
A
|
S
|
R
|
K
|
R
|
240 |
ICL3 | TM6 | ||||||||||
L
|
T
|
V
|
S
|
L
|
A
|
Y
|
S
|
E
|
S
|
||
250 |
H
|
Q
|
I
|
R
|
V
|
S
|
Q
|
Q
|
D
|
F
|
260 |
R
|
L
|
F
|
R
|
T
|
L
|
F
|
L
|
L
|
M
|
270 |
V
|
S
|
F
|
F
|
I
|
M
|
W
|
S
|
P
|
I
|
280 |
I
|
I
|
T
|
I
|
L
|
L
|
I
|
L
|
I
|
Q
|
290 |
ECL3 | |||||||||
N
|
F
|
K
|
Q
|
D
|
L
|
V
|
I
|
W
|
P
|
300 |
TM7 | |||||||||
S
|
L
|
F
|
F
|
W
|
V
|
V
|
A
|
F
|
T
|
310 |
F
|
A
|
N
|
S
|
A
|
L
|
N
|
P
|
I
|
L
|
320 |
|
|||||||||
H8 | |||||||||
Y
|
N
|
M
|
T
|
L
|
C
|
R
|
N
|
E
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
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DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
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Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|