grm2_human
NAME
8WGB
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
grm2_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
|
G
|
S
|
L
|
L
|
A
|
L
|
L
|
A
|
L
|
10 |
|
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||
L
|
L
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L
|
W
|
G
|
A
|
V
|
A
|
E
|
G
|
20 |
P
|
A
|
K
|
K
|
V
|
L
|
T
|
L
|
E
|
G
|
30 |
D
|
L
|
V
|
L
|
G
|
G
|
L
|
F
|
P
|
V
|
40 |
H
|
Q
|
K
|
G
|
G
|
P
|
A
|
E
|
D
|
C
|
50 |
G
|
P
|
V
|
N
|
E
|
H
|
R
|
G
|
I
|
Q
|
60 |
R
|
L
|
E
|
A
|
M
|
L
|
F
|
A
|
L
|
D
|
70 |
R
|
I
|
N
|
R
|
D
|
P
|
H
|
L
|
L
|
P
|
80 |
G
|
V
|
R
|
L
|
G
|
A
|
H
|
I
|
L
|
D
|
90 |
S
|
C
|
S
|
K
|
D
|
T
|
H
|
A
|
L
|
E
|
100 |
Q
|
A
|
L
|
D
|
F
|
V
|
R
|
A
|
S
|
L
|
110 |
S
|
R
|
G
|
A
|
D
|
G
|
S
|
R
|
H
|
I
|
120 |
C
|
P
|
D
|
G
|
S
|
Y
|
A
|
T
|
H
|
G
|
130 |
D
|
A
|
P
|
T
|
A
|
I
|
T
|
G
|
V
|
I
|
140 |
G
|
G
|
S
|
Y
|
S
|
D
|
V
|
S
|
I
|
Q
|
150 |
V
|
A
|
N
|
L
|
L
|
R
|
L
|
F
|
Q
|
I
|
160 |
P
|
Q
|
I
|
S
|
Y
|
A
|
S
|
T
|
S
|
A
|
170 |
K
|
L
|
S
|
D
|
K
|
S
|
R
|
Y
|
D
|
Y
|
180 |
F
|
A
|
R
|
T
|
V
|
P
|
P
|
D
|
F
|
F
|
190 |
Q
|
A
|
K
|
A
|
M
|
A
|
E
|
I
|
L
|
R
|
200 |
F
|
F
|
N
|
W
|
T
|
Y
|
V
|
S
|
T
|
V
|
210 |
A
|
S
|
E
|
G
|
D
|
Y
|
G
|
E
|
T
|
G
|
220 |
I
|
E
|
A
|
F
|
E
|
L
|
E
|
A
|
R
|
A
|
230 |
R
|
N
|
I
|
C
|
V
|
A
|
T
|
S
|
E
|
K
|
240 |
V
|
G
|
R
|
A
|
M
|
S
|
R
|
A
|
A
|
F
|
250 |
E
|
G
|
V
|
V
|
R
|
A
|
L
|
L
|
Q
|
K
|
260 |
P
|
S
|
A
|
R
|
V
|
A
|
V
|
L
|
F
|
T
|
270 |
R
|
S
|
E
|
D
|
A
|
R
|
E
|
L
|
L
|
A
|
280 |
A
|
S
|
Q
|
R
|
L
|
N
|
A
|
S
|
F
|
T
|
290 |
W
|
V
|
A
|
S
|
D
|
G
|
W
|
G
|
A
|
L
|
300 |
E
|
S
|
V
|
V
|
A
|
G
|
S
|
E
|
G
|
A
|
310 |
A
|
E
|
G
|
A
|
I
|
T
|
I
|
E
|
L
|
A
|
320 |
S
|
Y
|
P
|
I
|
S
|
D
|
F
|
A
|
S
|
Y
|
330 |
F
|
Q
|
S
|
L
|
D
|
P
|
W
|
N
|
N
|
S
|
340 |
R
|
N
|
P
|
W
|
F
|
R
|
E
|
F
|
W
|
E
|
350 |
Q
|
R
|
F
|
R
|
C
|
S
|
F
|
R
|
Q
|
R
|
360 |
D
|
C
|
A
|
A
|
H
|
S
|
L
|
R
|
A
|
V
|
370 |
P
|
F
|
E
|
Q
|
E
|
S
|
K
|
I
|
M
|
F
|
380 |
V
|
V
|
N
|
A
|
V
|
Y
|
A
|
M
|
A
|
H
|
390 |
A
|
L
|
H
|
N
|
M
|
H
|
R
|
A
|
L
|
C
|
400 |
P
|
N
|
T
|
T
|
R
|
L
|
C
|
D
|
A
|
M
|
410 |
R
|
P
|
V
|
N
|
G
|
R
|
R
|
L
|
Y
|
K
|
420 |
D
|
F
|
V
|
L
|
N
|
V
|
K
|
F
|
D
|
A
|
430 |
P
|
F
|
R
|
P
|
A
|
D
|
T
|
H
|
N
|
E
|
440 |
V
|
R
|
F
|
D
|
R
|
F
|
G
|
D
|
G
|
I
|
450 |
G
|
R
|
Y
|
N
|
I
|
F
|
T
|
Y
|
L
|
R
|
460 |
A
|
G
|
S
|
G
|
R
|
Y
|
R
|
Y
|
Q
|
K
|
470 |
V
|
G
|
Y
|
W
|
A
|
E
|
G
|
L
|
T
|
L
|
480 |
D
|
T
|
S
|
L
|
I
|
P
|
W
|
A
|
S
|
P
|
490 |
S
|
A
|
G
|
P
|
L
|
P
|
A
|
S
|
R
|
C
|
500 |
S
|
E
|
P
|
C
|
L
|
Q
|
N
|
E
|
V
|
K
|
510 |
S
|
V
|
Q
|
P
|
G
|
E
|
V
|
C
|
C
|
W
|
520 |
L
|
C
|
I
|
P
|
C
|
Q
|
P
|
Y
|
E
|
Y
|
530 |
R
|
L
|
D
|
E
|
F
|
T
|
C
|
A
|
D
|
C
|
540 |
G
|
L
|
G
|
Y
|
W
|
P
|
N
|
A
|
S
|
L
|
550 |
T
|
G
|
C
|
F
|
E
|
L
|
P
|
Q
|
E
|
Y
|
560 |
TM1 | |||||||||
I
|
R
|
W
|
G
|
D
|
A
|
W
|
A
|
V
|
G
|
570 |
P
|
V
|
T
|
I
|
A
|
C
|
L
|
G
|
A
|
L
|
580 |
A
|
T
|
L
|
F
|
V
|
L
|
G
|
V
|
F
|
V
|
590 |
ICL1 | |||||||||
R
|
H
|
N
|
A
|
T
|
P
|
V
|
V
|
K
|
A
|
600 |
TM2 | |||||||||
S
|
G
|
R
|
E
|
L
|
C
|
Y
|
I
|
L
|
L
|
610 |
G
|
G
|
V
|
F
|
L
|
C
|
Y
|
C
|
M
|
T
|
620 |
ECL1 | TM3 | ||||||||||
F
|
I
|
F
|
I
|
A
|
K
|
P
|
S
|
T
|
A
|
||
630 |
V
|
C
|
T
|
L
|
R
|
R
|
L
|
G
|
L
|
G
|
640 |
T
|
A
|
F
|
S
|
V
|
C
|
Y
|
S
|
A
|
L
|
650 |
L
|
T
|
K
|
T
|
N
|
R
|
I
|
A
|
R
|
I
|
660 |
ICL2 | |||||||||
F
|
G
|
G
|
A
|
R
|
E
|
G
|
A
|
Q
|
R
|
670 |
TM4 | |||||||||
P
|
R
|
F
|
I
|
S
|
P
|
A
|
S
|
Q
|
V
|
680 |
A
|
I
|
C
|
L
|
A
|
L
|
I
|
S
|
G
|
Q
|
690 |
L
|
L
|
I
|
V
|
V
|
A
|
W
|
L
|
V
|
V
|
700 |
ECL2 | |||||||||
E
|
A
|
P
|
G
|
T
|
G
|
K
|
E
|
T
|
A
|
710 |
P
|
E
|
R
|
R
|
E
|
V
|
V
|
T
|
L
|
R
|
720 |
TM5 | |||||||||
C
|
N
|
H
|
R
|
D
|
A
|
S
|
M
|
L
|
G
|
730 |
S
|
L
|
A
|
Y
|
N
|
V
|
L
|
L
|
I
|
A
|
740 |
L
|
C
|
T
|
L
|
Y
|
A
|
F
|
K
|
T
|
R
|
750 |
ICL3 | TM6 | ||||||||
K
|
C
|
P
|
E
|
N
|
F
|
N
|
E
|
A
|
K
|
760 |
F
|
I
|
G
|
F
|
T
|
M
|
Y
|
T
|
T
|
C
|
770 |
I
|
I
|
W
|
L
|
A
|
F
|
L
|
P
|
I
|
F
|
780 |
ECL3 | TM7 | |||||||||
Y
|
V
|
T
|
S
|
S
|
D
|
Y
|
R
|
V
|
Q
|
|
790 |
T
|
T
|
T
|
M
|
C
|
V
|
S
|
V
|
S
|
L
|
800 |
S
|
G
|
S
|
V
|
V
|
L
|
G
|
C
|
L
|
F
|
810 |
A
|
P
|
K
|
L
|
H
|
I
|
I
|
L
|
F
|
Q
|
820 |
H8 | |||||||||
P
|
Q
|
K
|
N
|
V
|
V
|
S
|
H
|
R
|
A
|
830 |
C-term | |||||||||
P
|
T
|
S
|
R
|
F
|
G
|
S
|
A
|
A
|
A
|
840 |
R
|
A
|
S
|
S
|
S
|
L
|
G
|
Q
|
G
|
S
|
850 |
G
|
S
|
Q
|
F
|
V
|
P
|
T
|
V
|
C
|
N
|
860 |
G
|
R
|
E
|
V
|
V
|
D
|
S
|
T
|
T
|
S
|
870 |
S
|
L
|
|
|||||||||
N-term | |||||||||
|
E
|
G
|
P
|
A
|
K
|
K
|
V
|
L
|
T
|
27 |
L
|
E
|
G
|
D
|
L
|
V
|
L
|
G
|
G
|
L
|
37 |
F
|
P
|
V
|
H
|
Q
|
K
|
G
|
G
|
P
|
A
|
47 |
E
|
D
|
C
|
G
|
P
|
V
|
N
|
E
|
H
|
R
|
57 |
G
|
I
|
Q
|
R
|
L
|
E
|
A
|
M
|
L
|
F
|
67 |
A
|
L
|
D
|
R
|
I
|
N
|
R
|
D
|
P
|
H
|
77 |
L
|
L
|
P
|
G
|
V
|
R
|
L
|
G
|
A
|
H
|
87 |
I
|
L
|
D
|
S
|
C
|
S
|
K
|
D
|
T
|
H
|
97 |
A
|
L
|
E
|
Q
|
A
|
L
|
D
|
F
|
V
|
R
|
107 |
A
|
S
|
L
|
S
|
R
|
G
|
A
|
D
|
G
|
S
|
117 |
R
|
H
|
I
|
C
|
P
|
D
|
G
|
S
|
Y
|
A
|
127 |
T
|
H
|
G
|
D
|
A
|
P
|
T
|
A
|
I
|
T
|
137 |
G
|
V
|
I
|
G
|
G
|
S
|
Y
|
S
|
D
|
V
|
147 |
S
|
I
|
Q
|
V
|
A
|
N
|
L
|
L
|
R
|
L
|
157 |
F
|
Q
|
I
|
P
|
Q
|
I
|
S
|
Y
|
A
|
S
|
167 |
T
|
S
|
A
|
K
|
L
|
S
|
D
|
K
|
S
|
R
|
177 |
Y
|
D
|
Y
|
F
|
A
|
R
|
T
|
V
|
P
|
P
|
187 |
D
|
F
|
F
|
Q
|
A
|
K
|
A
|
M
|
A
|
E
|
197 |
I
|
L
|
R
|
F
|
F
|
N
|
W
|
T
|
Y
|
V
|
207 |
S
|
T
|
V
|
A
|
S
|
E
|
G
|
D
|
Y
|
G
|
217 |
E
|
T
|
G
|
I
|
E
|
A
|
F
|
E
|
L
|
E
|
227 |
A
|
R
|
A
|
R
|
N
|
I
|
C
|
V
|
A
|
T
|
237 |
S
|
E
|
K
|
V
|
G
|
R
|
A
|
M
|
S
|
R
|
247 |
A
|
A
|
F
|
E
|
G
|
V
|
V
|
R
|
A
|
L
|
257 |
L
|
Q
|
K
|
P
|
S
|
A
|
R
|
V
|
A
|
V
|
267 |
L
|
F
|
T
|
R
|
S
|
E
|
D
|
A
|
R
|
E
|
277 |
L
|
L
|
A
|
A
|
S
|
Q
|
R
|
L
|
N
|
A
|
287 |
S
|
F
|
T
|
W
|
V
|
A
|
S
|
D
|
G
|
W
|
297 |
G
|
A
|
L
|
E
|
S
|
V
|
V
|
A
|
G
|
S
|
307 |
E
|
G
|
A
|
A
|
E
|
G
|
A
|
I
|
T
|
I
|
317 |
E
|
L
|
A
|
S
|
Y
|
P
|
I
|
S
|
D
|
F
|
327 |
A
|
S
|
Y
|
F
|
Q
|
S
|
L
|
D
|
P
|
W
|
337 |
N
|
N
|
S
|
R
|
N
|
P
|
W
|
F
|
R
|
E
|
347 |
F
|
W
|
E
|
Q
|
R
|
F
|
R
|
C
|
S
|
F
|
357 |
R
|
Q
|
R
|
D
|
C
|
A
|
A
|
H
|
S
|
L
|
367 |
R
|
A
|
V
|
P
|
F
|
E
|
Q
|
E
|
S
|
K
|
377 |
I
|
M
|
F
|
V
|
V
|
N
|
A
|
V
|
Y
|
A
|
387 |
M
|
A
|
H
|
A
|
L
|
H
|
N
|
M
|
H
|
R
|
397 |
A
|
L
|
C
|
P
|
N
|
T
|
T
|
R
|
L
|
C
|
407 |
D
|
A
|
M
|
R
|
P
|
V
|
N
|
G
|
R
|
R
|
417 |
L
|
Y
|
K
|
D
|
F
|
V
|
L
|
N
|
V
|
K
|
427 |
F
|
D
|
A
|
P
|
F
|
R
|
P
|
A
|
D
|
T
|
437 |
H
|
N
|
E
|
V
|
R
|
F
|
D
|
R
|
F
|
G
|
447 |
D
|
G
|
I
|
G
|
R
|
Y
|
N
|
I
|
F
|
T
|
457 |
Y
|
L
|
R
|
A
|
G
|
S
|
G
|
R
|
Y
|
R
|
467 |
Y
|
Q
|
K
|
V
|
G
|
Y
|
W
|
A
|
E
|
G
|
477 |
L
|
T
|
L
|
D
|
T
|
S
|
L
|
I
|
P
|
W
|
487 |
A
|
S
|
P
|
S
|
A
|
G
|
P
|
L
|
P
|
A
|
497 |
S
|
R
|
C
|
S
|
E
|
P
|
C
|
L
|
Q
|
N
|
507 |
E
|
V
|
K
|
S
|
V
|
Q
|
P
|
G
|
E
|
V
|
517 |
C
|
C
|
W
|
L
|
C
|
I
|
P
|
C
|
Q
|
P
|
527 |
Y
|
E
|
Y
|
R
|
L
|
D
|
E
|
F
|
T
|
C
|
537 |
A
|
D
|
C
|
G
|
L
|
G
|
Y
|
W
|
P
|
N
|
547 |
A
|
S
|
L
|
T
|
G
|
C
|
F
|
E
|
L
|
P
|
557 |
TM1 | |||||||||
Q
|
E
|
Y
|
I
|
R
|
W
|
G
|
D
|
A
|
W
|
567 |
A
|
V
|
G
|
P
|
V
|
T
|
I
|
A
|
C
|
L
|
577 |
G
|
A
|
L
|
A
|
T
|
L
|
F
|
V
|
L
|
G
|
587 |
ICL1 | |||||||||
V
|
F
|
V
|
R
|
H
|
N
|
A
|
T
|
P
|
V
|
597 |
TM2 | |||||||||
V
|
K
|
A
|
S
|
G
|
R
|
E
|
L
|
C
|
Y
|
607 |
I
|
L
|
L
|
G
|
G
|
V
|
F
|
L
|
C
|
Y
|
617 |
C
|
M
|
T
|
F
|
I
|
F
|
I
|
A
|
K
|
P
|
627 |
TM3 | |||||||||
S
|
T
|
A
|
V
|
C
|
T
|
L
|
R
|
R
|
L
|
637 |
G
|
L
|
G
|
T
|
A
|
F
|
S
|
V
|
C
|
Y
|
647 |
S
|
A
|
L
|
L
|
T
|
K
|
T
|
N
|
R
|
I
|
657 |
ICL2 | |||||||||
A
|
R
|
I
|
F
|
G
|
G
|
A
|
R
|
E
|
G
|
667 |
TM4 | |||||||||
A
|
Q
|
R
|
P
|
R
|
F
|
I
|
S
|
P
|
A
|
677 |
S
|
Q
|
V
|
A
|
I
|
C
|
L
|
A
|
L
|
I
|
687 |
S
|
G
|
Q
|
L
|
L
|
I
|
V
|
V
|
A
|
W
|
697 |
ECL2 | |||||||||
L
|
V
|
V
|
E
|
A
|
P
|
G
|
T
|
G
|
K
|
707 |
E
|
T
|
A
|
P
|
E
|
R
|
R
|
E
|
V
|
V
|
717 |
TM5 | |||||||||
T
|
L
|
R
|
C
|
N
|
H
|
R
|
D
|
A
|
S
|
727 |
M
|
L
|
G
|
S
|
L
|
A
|
Y
|
N
|
V
|
L
|
737 |
L
|
I
|
A
|
L
|
C
|
T
|
L
|
Y
|
A
|
F
|
747 |
ICL3 | |||||||||
K
|
T
|
R
|
K
|
C
|
P
|
E
|
N
|
F
|
N
|
757 |
TM6 | |||||||||
E
|
A
|
K
|
F
|
I
|
G
|
F
|
T
|
M
|
Y
|
767 |
T
|
T
|
C
|
I
|
I
|
W
|
L
|
A
|
F
|
L
|
777 |
P
|
I
|
F
|
Y
|
V
|
T
|
S
|
S
|
D
|
Y
|
787 |
TM7 | |||||||||
R
|
V
|
Q
|
T
|
T
|
T
|
M
|
C
|
V
|
S
|
797 |
V
|
S
|
L
|
S
|
G
|
S
|
V
|
V
|
L
|
G
|
807 |
C
|
L
|
F
|
A
|
P
|
K
|
L
|
H
|
I
|
I
|
817 |
H8 | |||||||||
L
|
F
|
Q
|
P
|
Q
|
K
|
N
|
V
|
V
|
S
|
827 |
H
|
R
|
A
|
P
|
T
|
S
|
R
|
F
|
G
|
S
|
837 |
C-term | |||||||||
A
|
A
|
A
|
R
|
A
|
S
|
S
|
S
|
L
|
G
|
847 |
Q
|
G
|
S
|
G
|
S
|
Q
|
F
|
V
|
P
|
T
|
857 |
V
|
C
|
N
|
G
|
R
|
E
|
V
|
V
|
D
|
S
|
867 |
T
|
T
|
S
|
S
|
L
|
872 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
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|
|
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|
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|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|