mc4r_human
NAME
8WKZ
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
mc4r_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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V
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N
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S
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T
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H
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R
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G
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M
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H
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10 |
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|||||
T
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S
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L
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H
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L
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W
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N
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R
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S
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S
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20 |
Y
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R
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L
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H
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S
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N
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A
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S
|
E
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S
|
30 |
L
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G
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K
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G
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Y
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S
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D
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G
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G
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C
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40 |
TM1 | |||||||||
Y
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E
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Q
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L
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F
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V
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S
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P
|
E
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V
|
50 |
F
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V
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T
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L
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G
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V
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I
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S
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L
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L
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60 |
E
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N
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I
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L
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V
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I
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V
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A
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I
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A
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70 |
ICL1 | TM2 | ||||||||
K
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N
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K
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N
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L
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H
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S
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P
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M
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Y
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80 |
F
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F
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I
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C
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S
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L
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A
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V
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A
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D
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90 |
M
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L
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V
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S
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V
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S
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N
|
G
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S
|
E
|
100 |
T
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I
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V
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I
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T
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L
|
L
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N
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S
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T
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110 |
ECL1 | TM3 | ||||||||
D
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T
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D
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A
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Q
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S
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F
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T
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V
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N
|
120 |
I
|
D
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N
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V
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I
|
D
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S
|
V
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I
|
C
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130 |
S
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S
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L
|
L
|
A
|
S
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I
|
C
|
S
|
L
|
140 |
L
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S
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I
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A
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V
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D
|
R
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Y
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F
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T
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150 |
ICL2 | |||||||||
I
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F
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Y
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A
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L
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Q
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Y
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H
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N
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I
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160 |
TM4 | |||||||||
M
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T
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V
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K
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R
|
V
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G
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I
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I
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I
|
170 |
S
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C
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I
|
W
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A
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A
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C
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T
|
V
|
S
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180 |
G
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I
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L
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F
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I
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I
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Y
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S
|
D
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S
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190 |
TM5 | |||||||||
S
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A
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V
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I
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I
|
C
|
L
|
I
|
T
|
M
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200 |
F
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F
|
T
|
M
|
L
|
A
|
L
|
M
|
A
|
S
|
210 |
L
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Y
|
V
|
H
|
M
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F
|
L
|
M
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A
|
R
|
220 |
|
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|
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||
L
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H
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I
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K
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R
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I
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A
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V
|
L
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P
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230 |
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|||||
ICL3 | TM6 | ||||||||
G
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T
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G
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A
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I
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R
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Q
|
G
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A
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N
|
240 |
M
|
K
|
G
|
A
|
I
|
T
|
L
|
T
|
I
|
L
|
250 |
I
|
G
|
V
|
F
|
V
|
V
|
C
|
W
|
A
|
P
|
260 |
F
|
F
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L
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H
|
L
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I
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F
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Y
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I
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S
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270 |
ECL3 | |||||||||
C
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P
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Q
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N
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P
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Y
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C
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V
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C
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F
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280 |
TM7 | |||||||||
M
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S
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H
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F
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N
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L
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Y
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L
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I
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L
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290 |
I
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M
|
C
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N
|
S
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I
|
I
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D
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P
|
L
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300 |
H8 | |||||||||
I
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Y
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A
|
L
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R
|
S
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Q
|
E
|
L
|
R
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310 |
K
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T
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F
|
K
|
E
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I
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I
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C
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C
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Y
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320 |
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C-term | |||||||||
P
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L
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G
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G
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L
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C
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D
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L
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S
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S
|
330 |
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R
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Y
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|||||||||
N-term | |||||||||
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W
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N
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R
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S
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S
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Y
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R
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L
|
H
|
24 |
S
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N
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A
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S
|
E
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S
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L
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G
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K
|
G
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34 |
Y
|
S
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D
|
G
|
G
|
C
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Y
|
E
|
Q
|
L
|
44 |
TM1 | |||||||||
F
|
V
|
S
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P
|
E
|
V
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F
|
V
|
T
|
L
|
54 |
G
|
V
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I
|
S
|
L
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L
|
E
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N
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I
|
L
|
64 |
V
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I
|
V
|
A
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I
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A
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K
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N
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K
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N
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74 |
ICL1 | TM2 | ||||||||
L
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H
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S
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P
|
M
|
Y
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F
|
F
|
I
|
C
|
84 |
S
|
L
|
A
|
V
|
A
|
D
|
M
|
L
|
V
|
S
|
94 |
V
|
S
|
N
|
G
|
S
|
E
|
T
|
I
|
V
|
I
|
104 |
ECL1 | |||||||||
T
|
L
|
L
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N
|
S
|
T
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D
|
T
|
D
|
A
|
114 |
TM3 | |||||||||
Q
|
S
|
F
|
T
|
V
|
N
|
I
|
D
|
N
|
V
|
124 |
I
|
D
|
S
|
V
|
I
|
C
|
S
|
S
|
L
|
L
|
134 |
A
|
S
|
I
|
C
|
S
|
L
|
L
|
S
|
I
|
A
|
144 |
V
|
D
|
R
|
Y
|
F
|
T
|
I
|
F
|
Y
|
A
|
154 |
ICL2 | TM4 | ||||||||
L
|
Q
|
Y
|
H
|
N
|
I
|
M
|
T
|
V
|
K
|
164 |
R
|
V
|
G
|
I
|
I
|
I
|
S
|
C
|
I
|
W
|
174 |
A
|
A
|
C
|
T
|
V
|
S
|
G
|
I
|
L
|
F
|
184 |
ECL2 | TM5 | |||||||||
I
|
I
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Y
|
S
|
D
|
S
|
S
|
A
|
V
|
I
|
|
194 |
I
|
C
|
L
|
I
|
T
|
M
|
F
|
F
|
T
|
M
|
204 |
L
|
A
|
L
|
M
|
A
|
S
|
L
|
Y
|
V
|
H
|
214 |
|
|||||||||
M
|
F
|
L
|
M
|
A
|
R
|
L
|
H
|
|
R
|
236 |
TM6 | |||||||||
Q
|
G
|
A
|
N
|
M
|
K
|
G
|
A
|
I
|
T
|
246 |
L
|
T
|
I
|
L
|
I
|
G
|
V
|
F
|
V
|
V
|
256 |
C
|
W
|
A
|
P
|
F
|
F
|
L
|
H
|
L
|
I
|
266 |
ECL3 | |||||||||
F
|
Y
|
I
|
S
|
C
|
P
|
Q
|
N
|
P
|
Y
|
276 |
TM7 | |||||||||
C
|
V
|
C
|
F
|
M
|
S
|
H
|
F
|
N
|
L
|
286 |
Y
|
L
|
I
|
L
|
I
|
M
|
C
|
N
|
S
|
I
|
296 |
I
|
D
|
P
|
L
|
I
|
Y
|
A
|
L
|
R
|
S
|
306 |
H8 | |||||||||
Q
|
E
|
L
|
R
|
K
|
T
|
F
|
K
|
E
|
I
|
316 |
|
||||
I
|
C
|
C
|
Y
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
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DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
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Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|