e7fel0_danre
NAME
8ZFJ
ORGANISM
Zebrafish (Danio rerio)
PROTEIN NAME
e7fel0_danre
SOURCE
TREMBL
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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A
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S
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L
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R
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G
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S
|
E
|
T
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M
|
10 |
C
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N
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I
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T
|
T
|
S
|
C
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N
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V
|
D
|
20 |
TM1 | |||||||||
S
|
N
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I
|
D
|
Q
|
F
|
F
|
Q
|
P
|
T
|
30 |
L
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Y
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I
|
I
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V
|
I
|
V
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L
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G
|
F
|
40 |
P
|
T
|
N
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C
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M
|
A
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L
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W
|
A
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A
|
50 |
ICL1 | |||||||||
Y
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M
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Q
|
V
|
K
|
Q
|
K
|
N
|
E
|
L
|
60 |
TM2 | |||||||||
G
|
I
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Y
|
L
|
M
|
N
|
L
|
S
|
I
|
A
|
70 |
D
|
L
|
L
|
Y
|
I
|
T
|
T
|
L
|
P
|
L
|
80 |
W
|
I
|
D
|
Y
|
F
|
V
|
H
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H
|
D
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N
|
90 |
ECL1 | TM3 | ||||||||
W
|
I
|
H
|
G
|
Q
|
V
|
S
|
C
|
K
|
L
|
100 |
F
|
G
|
F
|
I
|
F
|
Y
|
T
|
N
|
I
|
Y
|
110 |
V
|
S
|
I
|
A
|
F
|
L
|
C
|
C
|
I
|
S
|
120 |
V
|
D
|
R
|
Y
|
L
|
A
|
V
|
A
|
H
|
P
|
130 |
ICL2 | TM4 | ||||||||
L
|
K
|
F
|
A
|
K
|
V
|
R
|
R
|
V
|
K
|
140 |
T
|
A
|
L
|
L
|
V
|
S
|
A
|
I
|
V
|
W
|
150 |
L
|
T
|
E
|
I
|
V
|
A
|
N
|
S
|
A
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P
|
160 |
ECL2 | |||||||||
L
|
F
|
H
|
D
|
E
|
L
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F
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Q
|
D
|
R
|
170 |
F
|
N
|
H
|
T
|
F
|
C
|
F
|
E
|
K
|
Y
|
180 |
P
|
M
|
E
|
P
|
W
|
V
|
A
|
G
|
M
|
N
|
190 |
TM5 | |||||||||
L
|
Y
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R
|
T
|
F
|
L
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G
|
F
|
L
|
A
|
200 |
P
|
W
|
G
|
I
|
M
|
L
|
A
|
A
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Y
|
R
|
210 |
G
|
I
|
L
|
R
|
A
|
V
|
R
|
G
|
N
|
V
|
220 |
ICL3 | TM6 | ||||||||
S
|
T
|
E
|
R
|
Q
|
E
|
K
|
A
|
K
|
I
|
230 |
K
|
R
|
L
|
A
|
L
|
S
|
L
|
I
|
L
|
I
|
240 |
V
|
L
|
L
|
C
|
F
|
A
|
P
|
Y
|
H
|
V
|
250 |
L
|
L
|
L
|
W
|
R
|
S
|
V
|
L
|
F
|
L
|
260 |
ECL3 | TM7 | ||||||||
I
|
N
|
P
|
C
|
D
|
C
|
G
|
G
|
E
|
E
|
270 |
N
|
L
|
F
|
G
|
A
|
Y
|
H
|
V
|
T
|
L
|
280 |
A
|
L
|
T
|
S
|
L
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N
|
C
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V
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A
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D
|
290 |
H8 | |||||||||
P
|
I
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L
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Y
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C
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F
|
V
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N
|
E
|
G
|
300 |
A
|
R
|
H
|
D
|
V
|
G
|
R
|
A
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L
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A
|
310 |
T
|
L
|
L
|
G
|
L
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F
|
Q
|
R
|
G
|
K
|
320 |
C-term | |||||||||
S
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P
|
E
|
T
|
L
|
M
|
G
|
A
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S
|
I
|
330 |
|
|
||||||||
T
|
V
|
E
|
T
|
P
|
L
|
A
|
V
|
K
|
K
|
340 |
|
|
|
|
|
|
|
|
|
|
P
|
D
|
F
|
Y
|
S
|
E
|
V
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K
|
T
|
N
|
350 |
|
|
|
|
|
|
|
|
|
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A
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Y
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K
|
N
|
D
|
I
|
E
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V
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L
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K
|
360 |
|
|
|
|
|
|
|
|
|
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D
|
E
|
C
|
L
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Q
|
M
|
T
|
I
|
L
|
S
|
370 |
|
|
|
V
|
K
|
K
|
|
|||||||||
N-term | |||||||||
|
A
|
S
|
L
|
R
|
G
|
S
|
E
|
T
|
M
|
10 |
C
|
N
|
I
|
T
|
T
|
S
|
C
|
N
|
V
|
D
|
20 |
TM1 | |||||||||
S
|
N
|
I
|
D
|
Q
|
F
|
F
|
Q
|
P
|
T
|
30 |
L
|
Y
|
I
|
I
|
V
|
I
|
V
|
L
|
G
|
F
|
40 |
P
|
T
|
N
|
C
|
M
|
A
|
L
|
W
|
A
|
A
|
50 |
ICL1 | |||||||||
Y
|
M
|
Q
|
V
|
K
|
Q
|
K
|
N
|
E
|
L
|
60 |
TM2 | |||||||||
G
|
I
|
Y
|
L
|
M
|
N
|
L
|
S
|
I
|
A
|
70 |
D
|
L
|
L
|
Y
|
I
|
T
|
T
|
L
|
P
|
L
|
80 |
W
|
I
|
D
|
Y
|
F
|
V
|
H
|
H
|
D
|
N
|
90 |
ECL1 | TM3 | ||||||||
W
|
I
|
H
|
G
|
Q
|
V
|
S
|
C
|
K
|
L
|
100 |
F
|
G
|
F
|
I
|
F
|
Y
|
T
|
N
|
I
|
Y
|
110 |
V
|
S
|
I
|
A
|
F
|
L
|
C
|
C
|
I
|
S
|
120 |
V
|
D
|
R
|
Y
|
L
|
A
|
V
|
A
|
H
|
P
|
130 |
ICL2 | TM4 | ||||||||
L
|
K
|
F
|
A
|
K
|
V
|
R
|
R
|
V
|
K
|
140 |
T
|
A
|
L
|
L
|
V
|
S
|
A
|
I
|
V
|
W
|
150 |
L
|
T
|
E
|
I
|
V
|
A
|
N
|
S
|
A
|
P
|
160 |
ECL2 | |||||||||
L
|
F
|
H
|
D
|
E
|
L
|
F
|
Q
|
D
|
R
|
170 |
F
|
N
|
H
|
T
|
F
|
C
|
F
|
E
|
K
|
Y
|
180 |
P
|
M
|
E
|
P
|
W
|
V
|
A
|
G
|
M
|
N
|
190 |
TM5 | |||||||||
L
|
Y
|
R
|
T
|
F
|
L
|
G
|
F
|
L
|
A
|
200 |
P
|
W
|
G
|
I
|
M
|
L
|
A
|
A
|
Y
|
R
|
210 |
G
|
I
|
L
|
R
|
A
|
V
|
R
|
G
|
N
|
V
|
220 |
ICL3 | TM6 | ||||||||
S
|
T
|
E
|
R
|
Q
|
E
|
K
|
A
|
K
|
I
|
230 |
K
|
R
|
L
|
A
|
L
|
S
|
L
|
I
|
L
|
I
|
240 |
V
|
L
|
L
|
C
|
F
|
A
|
P
|
Y
|
H
|
V
|
250 |
L
|
L
|
L
|
W
|
R
|
S
|
V
|
L
|
F
|
L
|
260 |
ECL3 | TM7 | ||||||||
I
|
N
|
P
|
C
|
D
|
C
|
G
|
G
|
E
|
E
|
270 |
N
|
L
|
F
|
G
|
A
|
Y
|
H
|
V
|
T
|
L
|
280 |
A
|
L
|
T
|
S
|
L
|
N
|
C
|
V
|
A
|
D
|
290 |
H8 | |||||||||
P
|
I
|
L
|
Y
|
C
|
F
|
V
|
N
|
E
|
G
|
300 |
A
|
R
|
H
|
D
|
V
|
G
|
R
|
A
|
L
|
A
|
310 |
T
|
L
|
L
|
G
|
L
|
F
|
Q
|
R
|
G
|
K
|
320 |
C-term | |||||||||
S
|
P
|
E
|
T
|
L
|
M
|
G
|
A
|
S
|
I
|
330 |
|
||||||||
T
|
V
|
E
|
T
|
P
|
L
|
A
|
V
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|