b1b1u5_9arac
NAME
9EPR
ORGANISM
Hasarius adansoni (Hasarius adansoni)
PROTEIN NAME
b1b1u5_9arac
SOURCE
TREMBL
SEQUENCE
Perspective:
Wild-type
Construct
N-term | |||||||||
M
|
L
|
P
|
H
|
A
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A
|
K
|
M
|
A
|
A
|
10 |
R
|
V
|
A
|
G
|
D
|
H
|
D
|
G
|
R
|
N
|
20 |
I
|
S
|
I
|
V
|
D
|
L
|
L
|
P
|
E
|
D
|
30 |
M
|
L
|
P
|
M
|
I
|
H
|
E
|
H
|
W
|
Y
|
40 |
TM1 | |||||||||
K
|
F
|
P
|
P
|
M
|
E
|
T
|
S
|
M
|
H
|
50 |
Y
|
I
|
L
|
G
|
M
|
L
|
I
|
I
|
V
|
I
|
60 |
G
|
I
|
I
|
S
|
V
|
S
|
G
|
N
|
G
|
V
|
70 |
V
|
M
|
Y
|
L
|
M
|
M
|
T
|
V
|
K
|
N
|
80 |
ICL1 | TM2 | ||||||||
L
|
R
|
T
|
P
|
G
|
N
|
F
|
L
|
V
|
L
|
90 |
N
|
L
|
A
|
L
|
S
|
D
|
F
|
G
|
M
|
L
|
100 |
F
|
F
|
M
|
M
|
P
|
T
|
M
|
S
|
I
|
N
|
110 |
ECL1 | |||||||||
C
|
F
|
A
|
E
|
T
|
W
|
V
|
I
|
G
|
P
|
120 |
TM3 | |||||||||
F
|
M
|
C
|
E
|
L
|
Y
|
G
|
M
|
I
|
G
|
130 |
S
|
L
|
F
|
G
|
S
|
A
|
S
|
I
|
W
|
S
|
140 |
L
|
V
|
M
|
I
|
T
|
L
|
D
|
R
|
Y
|
N
|
150 |
ICL2 | |||||||||
V
|
I
|
V
|
K
|
G
|
M
|
A
|
G
|
K
|
P
|
160 |
TM4 | |||||||||
L
|
T
|
K
|
V
|
G
|
A
|
L
|
L
|
R
|
M
|
170 |
L
|
F
|
V
|
W
|
I
|
W
|
S
|
L
|
G
|
W
|
180 |
ECL2 | |||||||||
T
|
I
|
A
|
P
|
M
|
Y
|
G
|
W
|
S
|
R
|
190 |
Y
|
V
|
P
|
E
|
G
|
S
|
M
|
T
|
S
|
C
|
200 |
TM5 | |||||||||
T
|
I
|
D
|
Y
|
I
|
D
|
T
|
A
|
I
|
N
|
210 |
P
|
M
|
S
|
Y
|
L
|
I
|
A
|
Y
|
A
|
I
|
220 |
F
|
V
|
Y
|
F
|
V
|
P
|
L
|
F
|
I
|
I
|
230 |
I
|
Y
|
C
|
Y
|
A
|
F
|
I
|
V
|
M
|
Q
|
240 |
V
|
A
|
A
|
H
|
E
|
K
|
S
|
L
|
R
|
E
|
250 |
ICL3 | |||||||||
Q
|
A
|
K
|
K
|
M
|
N
|
I
|
K
|
S
|
L
|
260 |
TM6 | |||||||||
R
|
S
|
N
|
E
|
D
|
N
|
K
|
K
|
A
|
S
|
270 |
A
|
E
|
F
|
R
|
L
|
A
|
K
|
V
|
A
|
F
|
280 |
M
|
T
|
I
|
C
|
C
|
W
|
F
|
M
|
A
|
W
|
290 |
T
|
P
|
Y
|
L
|
T
|
L
|
S
|
F
|
L
|
G
|
300 |
ECL3 | |||||||||
I
|
F
|
S
|
D
|
R
|
T
|
W
|
L
|
T
|
P
|
310 |
TM7 | |||||||||
M
|
T
|
S
|
V
|
W
|
G
|
A
|
I
|
F
|
A
|
320 |
K
|
A
|
S
|
A
|
C
|
Y
|
N
|
P
|
I
|
V
|
330 |
H8 | |||||||||
Y
|
G
|
I
|
S
|
H
|
P
|
K
|
Y
|
R
|
A
|
340 |
A
|
L
|
H
|
D
|
K
|
F
|
P
|
C
|
L
|
K
|
350 |
C-term | |||||||||
C
|
G
|
S
|
D
|
S
|
P
|
K
|
G
|
D
|
S
|
360 |
A
|
S
|
T
|
V
|
A
|
E
|
S
|
E
|
K
|
A
|
370 |
G
|
E
|
N-term | |||||||||
M
|
L
|
P
|
H
|
A
|
A
|
K
|
M
|
A
|
A
|
10 |
R
|
V
|
A
|
G
|
D
|
H
|
D
|
G
|
R
|
N
|
20 |
I
|
S
|
I
|
V
|
D
|
L
|
L
|
P
|
E
|
D
|
30 |
M
|
L
|
P
|
M
|
I
|
H
|
E
|
H
|
W
|
Y
|
40 |
TM1 | |||||||||
K
|
F
|
P
|
P
|
M
|
E
|
T
|
S
|
M
|
H
|
50 |
Y
|
I
|
L
|
G
|
M
|
L
|
I
|
I
|
V
|
I
|
60 |
G
|
I
|
I
|
S
|
V
|
S
|
G
|
N
|
G
|
V
|
70 |
V
|
M
|
Y
|
L
|
M
|
M
|
T
|
V
|
K
|
N
|
80 |
ICL1 | TM2 | ||||||||
L
|
R
|
T
|
P
|
G
|
N
|
F
|
L
|
V
|
L
|
90 |
N
|
L
|
A
|
L
|
S
|
D
|
F
|
G
|
M
|
L
|
100 |
F
|
F
|
M
|
M
|
P
|
T
|
M
|
S
|
I
|
N
|
110 |
ECL1 | |||||||||
C
|
F
|
A
|
E
|
T
|
W
|
V
|
I
|
G
|
P
|
120 |
TM3 | |||||||||
F
|
M
|
C
|
E
|
L
|
Y
|
G
|
M
|
I
|
G
|
130 |
S
|
L
|
F
|
G
|
S
|
A
|
S
|
I
|
W
|
S
|
140 |
L
|
V
|
M
|
I
|
T
|
L
|
D
|
R
|
Y
|
N
|
150 |
ICL2 | |||||||||
V
|
I
|
V
|
K
|
G
|
M
|
A
|
G
|
K
|
P
|
160 |
TM4 | |||||||||
L
|
T
|
K
|
V
|
G
|
A
|
L
|
L
|
R
|
M
|
170 |
L
|
F
|
V
|
W
|
I
|
W
|
S
|
L
|
G
|
W
|
180 |
ECL2 | |||||||||
T
|
I
|
A
|
P
|
M
|
Y
|
G
|
W
|
S
|
R
|
190 |
Y
|
V
|
P
|
E
|
G
|
S
|
M
|
T
|
S
|
C
|
200 |
TM5 | |||||||||
T
|
I
|
D
|
Y
|
I
|
D
|
T
|
A
|
I
|
N
|
210 |
P
|
M
|
S
|
Y
|
L
|
I
|
A
|
Y
|
A
|
I
|
220 |
F
|
V
|
Y
|
F
|
V
|
P
|
L
|
F
|
I
|
I
|
230 |
I
|
Y
|
C
|
Y
|
A
|
F
|
I
|
V
|
M
|
Q
|
240 |
V
|
A
|
A
|
H
|
E
|
K
|
S
|
L
|
R
|
E
|
250 |
ICL3 | |||||||||
Q
|
A
|
K
|
K
|
M
|
N
|
I
|
K
|
S
|
L
|
260 |
TM6 | |||||||||
R
|
S
|
N
|
E
|
D
|
N
|
K
|
K
|
A
|
S
|
270 |
A
|
E
|
F
|
R
|
L
|
A
|
K
|
V
|
A
|
F
|
280 |
M
|
T
|
I
|
C
|
C
|
W
|
F
|
M
|
A
|
W
|
290 |
T
|
P
|
Y
|
L
|
T
|
L
|
S
|
F
|
L
|
G
|
300 |
ECL3 | |||||||||
I
|
F
|
S
|
D
|
R
|
T
|
W
|
L
|
T
|
P
|
310 |
TM7 | |||||||||
M
|
T
|
S
|
V
|
W
|
G
|
A
|
I
|
F
|
A
|
320 |
K
|
A
|
S
|
A
|
C
|
Y
|
N
|
P
|
I
|
V
|
330 |
H8 | |||||||||
Y
|
G
|
I
|
S
|
H
|
P
|
K
|
Y
|
R
|
A
|
340 |
A
|
L
|
H
|
D
|
K
|
F
|
P
|
C
|
L
|
K
|
350 |
C-term | |||||||||
C
|
G
|
S
|
D
|
S
|
P
|
K
|
G
|
D
|
S
|
360 |
A
|
S
|
T
|
V
|
A
|
E
|
S
|
E
|
K
|
A
|
370 |
G
|
E
|
372 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
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|
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|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|