STE2 - GPa1

GPCR CLASS (FAMILY)

Class D1 (Ste2-like fungal pheromone)

GPCR SPECIES

Saccharomyces cerevisiae

GPCR PREFERRED CHAIN

A

G PROTEIN (FAMILY)

GPa1 family

G PROTEIN SPECIES

Saccharomyces cerevisiae

PDB CODE

7AD3

RESOLUTION

3.5 Å

REFERENCE

10.1038/S41586-020-2994-1

PUBLICATION DATE

Dec. 9, 2020

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

82 interactions (Click to see)

LIGANDS

Alpha-factor mating pheromone - Agonist pubchem

ENDOGENOUS LIGANDS

No endogenous ligands available



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM2 T 78 2x40 G.H5 G 470 G.H5.24
Hydrophobic TM3 T 155 3x56 G.H5 I 461 G.H5.15
Hydrophobic ICL2 G 156 - G.H5 I 461 G.H5.15
Van-der-waals ICL2 G 156 - G.H5 I 461 G.H5.15
Polar ICL2 N 158 - G.s2s3 G 311 G.s2s3.01
Hydrophobic ICL2 N 158 - G.s2s3 G 311 G.s2s3.01
Van-der-waals ICL2 N 158 - G.s2s3 G 311 G.s2s3.01
Polar ICL2 K 160 - G.s2s3 S 312 G.s2s3.02
Hydrophobic ICL2 K 160 - G.s2s3 S 312 G.s2s3.02
Van-der-waals ICL2 K 160 - G.s2s3 S 312 G.s2s3.02
Hydrophobic TM6 F 244 6x36 G.H5 Q 463 G.H5.17
Van-der-waals TM6 F 244 6x36 G.H5 Q 463 G.H5.17
Hydrophobic TM3 V 152 3x53 G.H5 I 469 G.H5.23
Van-der-waals TM3 V 152 3x53 G.H5 I 469 G.H5.23
Hydrophobic ICL2 N 158 - G.S2 I 310 G.S2.08
Polar TM2 T 78 2x40 G.H5 I 469 G.H5.23
Hydrophobic TM2 T 78 2x40 G.H5 I 469 G.H5.23
Van-der-waals TM2 T 78 2x40 G.H5 I 469 G.H5.23
Hydrophobic ICL2 N 158 - G.S3 F 315 G.S3.03
Van-der-waals ICL2 N 158 - G.S3 F 315 G.S3.03
Hydrophobic TM5 F 235 5x59 G.H5 Q 448 G.H5.02
Van-der-waals TM5 F 235 5x59 G.H5 Q 448 G.H5.02
Hydrophobic TM3 V 152 3x53 G.H5 L 466 G.H5.20
Van-der-waals TM3 V 152 3x53 G.H5 L 466 G.H5.20
Hydrophobic TM5 L 236 5x60 G.S6 Y 438 G.S6.02
Van-der-waals TM5 L 236 5x60 G.S6 Y 438 G.S6.02
Hydrophobic TM6 F 244 6x36 G.H5 I 462 G.H5.16
Van-der-waals TM6 F 244 6x36 G.H5 I 462 G.H5.16
Hydrophobic TM5 L 236 5x60 G.H5 S 455 G.H5.09
Van-der-waals TM5 L 236 5x60 G.H5 S 455 G.H5.09
Hydrophobic TM2 I 80 2x42 G.H5 I 471 G.H5.25
Hydrophobic TM6 L 247 6x39 G.H5 I 471 G.H5.25
Van-der-waals ICL2 K 160 - G.S3 S 313 G.S3.01
Polar TM2 P 79 2x41 G.H5 I 469 G.H5.23
Hydrophobic TM2 P 79 2x41 G.H5 I 469 G.H5.23
Hydrophobic TM2 I 80 2x42 G.H5 G 470 G.H5.24
Van-der-waals TM2 I 80 2x42 G.H5 G 470 G.H5.24
Hydrophobic ICL2 F 159 - G.s2s3 G 311 G.s2s3.01
Van-der-waals ICL2 F 159 - G.s2s3 G 311 G.s2s3.01
Polar ICL2 N 158 - G.S3 S 313 G.S3.01
Hydrophobic ICL2 N 158 - G.S3 S 313 G.S3.01
Van-der-waals ICL2 N 158 - G.S3 S 313 G.S3.01
Hydrophobic TM7 K 304 7x64 G.h4s6 N 434 G.h4s6.10
Hydrophobic TM6 L 247 6x39 G.H5 I 472 G.H5.26
Ionic TM5 R 233 5x57 G.H5 D 459 G.H5.13
Polar TM5 R 233 5x57 G.H5 D 459 G.H5.13
Van-der-waals TM5 R 233 5x57 G.H5 D 459 G.H5.13
Hydrophobic TM7 L 289 7x49 G.H5 I 471 G.H5.25
Hydrophobic TM3 I 153 3x54 G.H5 I 462 G.H5.16
Hydrophobic TM3 Q 149 3x50 G.H5 I 471 G.H5.25
Hydrophobic TM5 L 236 5x60 G.H5 F 452 G.H5.06
Van-der-waals TM5 L 236 5x60 G.H5 F 452 G.H5.06
Hydrophobic ICL3 L 238 - G.S6 Y 438 G.S6.02
Van-der-waals ICL3 L 238 - G.S6 Y 438 G.S6.02
Polar TM5 S 232 5x56 G.H5 S 455 G.H5.09
Van-der-waals TM5 S 232 5x56 G.H5 S 455 G.H5.09
Polar TM2 I 80 2x42 G.H5 I 469 G.H5.23
Hydrophobic TM2 I 80 2x42 G.H5 I 469 G.H5.23
Van-der-waals TM2 I 80 2x42 G.H5 I 469 G.H5.23
Hydrophobic ICL2 D 157 - G.H5 T 458 G.H5.12
Hydrophobic ICL2 G 156 - G.H5 T 458 G.H5.12
Van-der-waals ICL2 G 156 - G.H5 T 458 G.H5.12
Hydrophobic TM6 S 243 6x35 G.H5 I 472 G.H5.26
Hydrophobic ICL2 F 159 - G.S2 I 310 G.S2.08
Van-der-waals ICL2 F 159 - G.S2 I 310 G.S2.08
Hydrophobic TM7 A 296 7x56 G.H5 I 472 G.H5.26
Hydrophobic TM3 V 152 3x53 G.H5 N 465 G.H5.19
Van-der-waals TM3 V 152 3x53 G.H5 N 465 G.H5.19
Hydrophobic TM3 I 153 3x54 G.H5 L 466 G.H5.20
Hydrophobic TM5 L 236 5x60 G.H5 A 456 G.H5.10
Polar ICL2 N 158 - G.s2s3 S 312 G.s2s3.02
Van-der-waals ICL2 N 158 - G.s2s3 S 312 G.s2s3.02
Hydrophobic TM3 F 148 3x49 G.H5 I 469 G.H5.23
Van-der-waals TM3 F 148 3x49 G.H5 I 469 G.H5.23
Polar TM3 T 155 3x56 G.H5 N 465 G.H5.19
Van-der-waals TM3 T 155 3x56 G.H5 N 465 G.H5.19
Aromatic TM5 F 235 5x59 G.H5 K 451 G.H5.05
Hydrophobic TM5 F 235 5x59 G.H5 K 451 G.H5.05
Polar TM5 R 233 5x57 G.H5 Q 463 G.H5.17
Hydrophobic TM5 R 233 5x57 G.H5 Q 463 G.H5.17
Van-der-waals TM5 R 233 5x57 G.H5 Q 463 G.H5.17