β2-adrenoceptor - Gs

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8GEF

RESOLUTION

3.8 Å

REFERENCE

10.1038/S41586-024-07153-1

PUBLICATION DATE

March 6, 2024

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

34 interactions (Click to see)

LIGANDS

(5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol - Agonist pubchem

PHYSIOLOGICAL LIGANDS

L-NOREPINEPHRINE, (-)-adrenaline, noradrenaline, Zn2+, (-)-noradrenaline



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic ICL2 F 139 34x51 G.S3 V 217 G.S3.01
Ionic TM5 K 232 5x71 G.H5 D 381 G.H5.13
Polar TM5 K 232 5x71 G.H5 D 381 G.H5.13
Hydrophobic TM5 K 232 5x71 G.H5 D 381 G.H5.13
Van-der-waals TM5 K 232 5x71 G.H5 D 381 G.H5.13
Hydrophobic TM5 Q 229 5x68 G.H5 L 388 G.H5.20
Van-der-waals TM5 Q 229 5x68 G.H5 L 388 G.H5.20
Hydrophobic TM3 I 135 3x54 G.H5 H 387 G.H5.19
Van-der-waals TM3 I 135 3x54 G.H5 H 387 G.H5.19
Hydrophobic TM5 I 233 5x72 G.h4s6 Y 358 G.h4s6.20
Van-der-waals TM5 I 233 5x72 G.h4s6 Y 358 G.h4s6.20
Polar TM5 Q 229 5x68 G.H5 Q 384 G.H5.16
Hydrophobic TM5 Q 229 5x68 G.H5 Q 384 G.H5.16
Van-der-waals TM5 Q 229 5x68 G.H5 Q 384 G.H5.16
Hydrophobic ICL2 P 138 34x50 G.H5 R 380 G.H5.12
Hydrophobic ICL2 P 138 34x50 G.H5 I 383 G.H5.15
Van-der-waals ICL2 P 138 34x50 G.H5 I 383 G.H5.15
Hydrophobic ICL2 F 139 34x51 G.H5 I 383 G.H5.15
Hydrophobic TM5 I 233 5x72 G.H5 R 385 G.H5.17
Van-der-waals TM5 I 233 5x72 G.H5 R 385 G.H5.17
Polar TM5 Q 229 5x68 G.H5 R 385 G.H5.17
Hydrophobic TM5 Q 229 5x68 G.H5 R 385 G.H5.17
Van-der-waals TM5 Q 229 5x68 G.H5 R 385 G.H5.17
Polar TM5 S 236 5x75 G.H4 R 342 G.H4.12
Hydrophobic ICL2 F 139 34x51 G.H5 F 376 G.H5.08
Van-der-waals ICL2 F 139 34x51 G.H5 F 376 G.H5.08
Hydrophobic TM6 L 275 6x37 G.H5 L 393 G.H5.25
Van-der-waals TM6 L 275 6x37 G.H5 L 393 G.H5.25
Polar TM6 A 271 6x33 G.H5 L 393 G.H5.25
Hydrophobic TM6 A 271 6x33 G.H5 L 393 G.H5.25
Van-der-waals TM6 A 271 6x33 G.H5 L 393 G.H5.25
Hydrophobic ICL2 F 139 34x51 G.H5 C 379 G.H5.11
Hydrophobic TM5 A 226 5x65 G.H5 L 388 G.H5.20
Van-der-waals TM5 A 226 5x65 G.H5 L 388 G.H5.20
Aromatic ICL2 F 139 34x51 G.S1 H 41 G.S1.02
Hydrophobic ICL2 F 139 34x51 G.S1 H 41 G.S1.02
Van-der-waals ICL2 F 139 34x51 G.S1 H 41 G.S1.02
Hydrophobic TM6 T 274 6x36 G.H5 E 392 G.H5.24
Polar TM3 A 134 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM3 A 134 3x53 G.H5 H 387 G.H5.19
Van-der-waals TM3 A 134 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM5 V 222 5x61 G.H5 L 393 G.H5.25
Van-der-waals TM5 V 222 5x61 G.H5 L 393 G.H5.25
Hydrophobic TM3 I 135 3x54 G.H5 L 388 G.H5.20
Van-der-waals TM3 I 135 3x54 G.H5 L 388 G.H5.20
Hydrophobic ICL2 F 139 34x51 G.H5 R 380 G.H5.12
Van-der-waals ICL2 F 139 34x51 G.H5 R 380 G.H5.12
Polar TM5 Q 229 5x68 G.H5 D 381 G.H5.13
Hydrophobic TM5 Q 229 5x68 G.H5 D 381 G.H5.13
Van-der-waals TM5 Q 229 5x68 G.H5 D 381 G.H5.13
Hydrophobic ICL2 F 139 34x51 G.S3 F 219 G.S3.03
Aromatic TM3 R 131 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM3 R 131 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM6 K 270 6x32 G.H5 L 394 G.H5.26
Hydrophobic ICL2 Y 141 34x53 G.H5 H 387 G.H5.19