ORF74 - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Human herpesvirus 8

GPCR PREFERRED CHAIN

E

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8K4O

RESOLUTION

3.0 Å

REFERENCE

PUBLICATION DATE

July 24, 2024

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

109 interactions (Click to see)

LIGANDS

Growth-regulated alpha protein - Agonist

PHYSIOLOGICAL LIGANDS

No physiological ligands available



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic ICL3 A 245 - G.H5 F 354 G.H5.26
Hydrophobic ICL3 T 241 - G.H5 D 341 G.H5.13
Hydrophobic ICL3 L 243 - G.H5 L 348 G.H5.20
Hydrophobic ICL3 L 243 - G.H5 I 344 G.H5.16
Hydrophobic TM3 L 146 3x53 G.H5 C 351 G.H5.23
Van-der-waals TM3 L 146 3x53 G.H5 C 351 G.H5.23
Hydrophobic ICL3 L 243 - G.h4s6 E 318 G.h4s6.12
Hydrophobic TM3 R 143 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM6 I 253 6x37 G.H5 L 353 G.H5.25
Hydrophobic ICL3 K 242 - G.S6 Y 320 G.S6.02
Polar ICL2 S 153 - G.HN E 28 G.HN.52
Polar ICL2 S 150 - G.H5 N 347 G.H5.19
Hydrophobic ICL2 S 150 - G.H5 N 347 G.H5.19
Van-der-waals ICL2 S 150 - G.H5 N 347 G.H5.19
Polar ICL3 Q 244 - G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL3 Q 244 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 Q 244 - G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL3 K 248 - G.H5 F 354 G.H5.26
Hydrophobic TM6 V 249 6x33 G.H5 F 354 G.H5.26
Hydrophobic TM5 V 238 5x65 G.H5 I 344 G.H5.16
Hydrophobic ICL2 T 151 - G.S3 L 194 G.S3.01
Hydrophobic ICL3 Q 244 - G.h4s6 D 315 G.h4s6.09
Van-der-waals ICL3 Q 244 - G.h4s6 D 315 G.h4s6.09
Hydrophobic ICL2 S 150 - G.H5 I 343 G.H5.15
Hydrophobic TM5 V 238 5x65 G.H5 L 348 G.H5.20
Van-der-waals TM5 V 238 5x65 G.H5 L 348 G.H5.20
Hydrophobic ICL2 T 151 - G.H5 I 343 G.H5.15
Hydrophobic ICL3 T 241 - G.H5 I 344 G.H5.16
Polar ICL3 K 242 - G.H5 D 341 G.H5.13
Hydrophobic TM2 I 82 2x39 G.H5 C 351 G.H5.23
Van-der-waals TM2 I 82 2x39 G.H5 C 351 G.H5.23
Polar TM7 G 318 7x57 G.H5 G 352 G.H5.24
Hydrophobic TM7 G 318 7x57 G.H5 G 352 G.H5.24
Van-der-waals TM7 G 318 7x57 G.H5 G 352 G.H5.24
Polar TM3 L 146 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 L 146 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 L 146 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL2 S 150 - G.H5 I 344 G.H5.16
Hydrophobic ICL3 L 243 - G.H5 K 345 G.H5.17
Van-der-waals ICL3 L 243 - G.H5 K 345 G.H5.17
Hydrophobic TM3 R 143 3x50 G.H5 L 353 G.H5.25
Hydrophobic TM3 V 147 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 V 147 3x54 G.H5 L 348 G.H5.20
Hydrophobic TM6 V 252 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM6 V 249 6x33 G.H5 L 353 G.H5.25
Hydrophobic ICL3 L 243 - G.H5 D 341 G.H5.13
Van-der-waals ICL3 L 243 - G.H5 D 341 G.H5.13
Polar ICL2 S 153 - G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 S 153 - G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 S 153 - G.hns1 R 32 G.hns1.03
Hydrophobic TM6 V 249 6x33 G.H5 L 348 G.H5.20