Gi2 - M4 receptor complex model

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

Gi/o

DATE

2023-09-15

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.


Tertiary structure confidence

(pLDDT score) avg.: 89.5

Very high (>90)
Confident (90-70)
Low (70-50)
Very low (<50)
Disordered

PTM Score: 0.8
iPTM Score: 0.8
PAE mean: 11.4


GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic ICL2 P 141 34x54 G.H5 N 348 G.H5.19
Hydrophobic TM6 R 394 6x29 G.H5 I 345 G.H5.16
Van-der-waals TM6 R 394 6x29 G.H5 I 345 G.H5.16
Polar TM5 R 225 5x68 G.H5 D 342 G.H5.13
Hydrophobic TM5 R 225 5x68 G.H5 D 342 G.H5.13
Van-der-waals TM5 R 225 5x68 G.H5 D 342 G.H5.13
Ionic TM5 H 229 5x72 G.H5 D 338 G.H5.09
Polar TM5 H 229 5x72 G.H5 D 338 G.H5.09
Hydrophobic TM5 H 229 5x72 G.H5 D 338 G.H5.09
Van-der-waals TM5 H 229 5x72 G.H5 D 338 G.H5.09
Hydrophobic TM6 R 394 6x29 G.H5 L 349 G.H5.20
Van-der-waals TM6 R 394 6x29 G.H5 L 349 G.H5.20
Polar TM6 T 401 6x36 G.H5 L 354 G.H5.25
Hydrophobic TM6 T 401 6x36 G.H5 L 354 G.H5.25
Hydrophobic TM7 C 456 7x56 G.H5 G 353 G.H5.24
Van-der-waals TM7 C 456 7x56 G.H5 G 353 G.H5.24
Hydrophobic ICL2 L 138 34x51 G.H5 F 337 G.H5.08
Polar ICL2 A 142 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 A 142 34x55 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 A 142 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 L 138 34x51 G.S3 L 195 G.S3.01
Van-der-waals ICL2 L 138 34x51 G.S3 L 195 G.S3.01
Hydrophobic TM5 H 229 5x72 G.H5 Q 334 G.H5.05
Hydrophobic ICL2 P 137 34x50 G.H5 I 344 G.H5.15
Van-der-waals ICL2 P 137 34x50 G.H5 I 344 G.H5.15
Hydrophobic TM5 S 222 5x65 G.H5 L 349 G.H5.20
Hydrophobic TM5 R 225 5x68 G.H5 I 345 G.H5.16
Hydrophobic TM5 V 226 5x69 G.H5 F 335 G.H5.06
Van-der-waals TM5 V 226 5x69 G.H5 F 335 G.H5.06
Hydrophobic ICL3 P 234 - G.H4 D 298 G.H4.04
Van-der-waals ICL3 P 234 - G.H4 D 298 G.H4.04
Ionic ICL3 R 386 - G.H4 D 310 G.H4.17
Polar ICL3 R 386 - G.H4 D 310 G.H4.17
Van-der-waals ICL3 R 386 - G.H4 D 310 G.H4.17
Polar TM6 Q 390 6x25 G.h4s6 K 318 G.h4s6.11
Van-der-waals TM6 Q 390 6x25 G.h4s6 K 318 G.h4s6.11
Polar TM3 C 133 3x53 G.H5 N 348 G.H5.19
Hydrophobic TM3 C 133 3x53 G.H5 N 348 G.H5.19
Van-der-waals TM3 C 133 3x53 G.H5 N 348 G.H5.19
Polar TM6 K 397 6x32 G.H5 F 355 G.H5.26
Aromatic TM6 K 397 6x32 G.H5 F 355 G.H5.26
Hydrophobic TM6 K 397 6x32 G.H5 F 355 G.H5.26
Van-der-waals TM6 K 397 6x32 G.H5 F 355 G.H5.26
Hydrophobic ICL2 P 137 34x50 G.H5 T 341 G.H5.12
Hydrophobic ICL3 R 386 - G.h4s6 K 315 G.h4s6.08
Van-der-waals ICL3 R 386 - G.h4s6 K 315 G.h4s6.08
Hydrophobic TM5 H 229 5x72 G.H5 F 335 G.H5.06
Ionic ICL3 K 387 - G.H4 E 309 G.H4.16
Polar ICL3 K 387 - G.H4 E 309 G.H4.16
Hydrophobic ICL3 K 387 - G.H4 E 309 G.H4.16
Van-der-waals ICL3 K 387 - G.H4 E 309 G.H4.16
Polar TM3 R 130 3x50 G.H5 C 352 G.H5.23
Hydrophobic TM3 R 130 3x50 G.H5 C 352 G.H5.23
Van-der-waals TM3 R 130 3x50 G.H5 C 352 G.H5.23
Polar TM6 R 394 6x29 G.H5 D 342 G.H5.13
Van-der-waals TM6 R 394 6x29 G.H5 D 342 G.H5.13
Polar ICL2 P 137 34x50 G.H5 N 348 G.H5.19
Van-der-waals ICL2 P 137 34x50 G.H5 N 348 G.H5.19
Hydrophobic TM5 V 226 5x69 G.H5 D 338 G.H5.09
Hydrophobic TM6 I 402 6x37 G.H5 L 354 G.H5.25
Hydrophobic TM5 I 218 5x61 G.H5 L 354 G.H5.25
Hydrophobic ICL2 P 137 34x50 G.H5 I 345 G.H5.16
Van-der-waals ICL2 P 137 34x50 G.H5 I 345 G.H5.16
Hydrophobic TM3 C 133 3x53 G.H5 C 352 G.H5.23
Hydrophobic TM3 R 130 3x50 G.H5 L 354 G.H5.25
Ionic C-term R 479 - G.h2s4 E 217 G.h2s4.02
Polar C-term R 479 - G.h2s4 E 217 G.h2s4.02
Van-der-waals C-term R 479 - G.h2s4 E 217 G.h2s4.02
Polar ICL3 K 387 - G.S6 T 322 G.S6.03
Van-der-waals ICL3 K 387 - G.S6 T 322 G.S6.03
Polar TM5 S 224 5x67 G.H5 D 342 G.H5.13
Hydrophobic TM5 S 224 5x67 G.H5 D 342 G.H5.13
Van-der-waals TM5 S 224 5x67 G.H5 D 342 G.H5.13
Hydrophobic TM5 R 225 5x68 G.H5 T 341 G.H5.12
Hydrophobic ICL2 L 138 34x51 G.H5 I 344 G.H5.15
Van-der-waals ICL2 L 138 34x51 G.H5 I 344 G.H5.15
Hydrophobic TM5 V 226 5x69 G.S6 Y 321 G.S6.02
Van-der-waals TM5 V 226 5x69 G.S6 Y 321 G.S6.02
Polar TM5 V 226 5x69 G.H5 D 342 G.H5.13
Hydrophobic TM5 V 226 5x69 G.H5 D 342 G.H5.13
Van-der-waals TM5 V 226 5x69 G.H5 D 342 G.H5.13
Hydrophobic TM4 T 145 4x38 G.HN E 28 G.HN.52
Polar ICL3 K 387 - G.S6 I 320 G.S6.01
Hydrophobic ICL3 K 387 - G.S6 I 320 G.S6.01
Van-der-waals ICL3 K 387 - G.S6 I 320 G.S6.01
Hydrophobic TM3 V 134 3x54 G.H5 I 345 G.H5.16
Hydrophobic TM6 R 394 6x29 G.H5 K 346 G.H5.17
Van-der-waals TM6 R 394 6x29 G.H5 K 346 G.H5.17
Hydrophobic TM6 V 398 6x33 G.H5 L 349 G.H5.20
Polar TM6 Q 390 6x25 G.h4s6 K 315 G.h4s6.08
Hydrophobic TM6 Q 390 6x25 G.h4s6 K 315 G.h4s6.08
Van-der-waals TM6 Q 390 6x25 G.h4s6 K 315 G.h4s6.08
Polar ICL3 N 383 - G.H4 E 309 G.H4.16
Van-der-waals ICL3 N 383 - G.H4 E 309 G.H4.16
Hydrophobic TM6 I 405 6x40 G.H5 L 354 G.H5.25
Hydrophobic TM5 V 226 5x69 G.H5 A 339 G.H5.10
Ionic ICL3 K 238 - G.H4 D 298 G.H4.04
Polar ICL3 K 238 - G.H4 D 298 G.H4.04
Hydrophobic ICL3 K 238 - G.H4 D 298 G.H4.04
Van-der-waals ICL3 K 238 - G.H4 D 298 G.H4.04
Hydrophobic TM6 R 394 6x29 G.H5 F 355 G.H5.26
Hydrophobic TM3 V 134 3x54 G.H5 L 349 G.H5.20
Van-der-waals TM3 V 134 3x54 G.H5 L 349 G.H5.20
Polar TM2 N 67 2x39 G.H5 C 352 G.H5.23
Hydrophobic TM2 N 67 2x39 G.H5 C 352 G.H5.23
Van-der-waals TM2 N 67 2x39 G.H5 C 352 G.H5.23