Refined structure (8GEB) of Gs - β2-adrenoceptor

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

Gs

DATE

2024-11-26

3D VIEW

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Structure
GPCR

Model
AlphaFold2-Multimer

REFINED G PROTEIN RESIDUES

Residue backbones - 233 from structure (59.1%), 161 from model (40.9%)
Residue sidechains - 156 from structure (39.6%), 238 from model (60.4%)

REFINED GPCR RESIDUES

Residue backbones - 281 from structure (82.4%), 60 from model (17.6%)
Residue sidechains - 194 from structure (56.9%), 147 from model (43.1%)



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:
H02G.S1W17G.H3R01G.h3s5Y08D13G.hgh4D13G.H4C01G.S6R12Q16R17H19L25G.H5GproteinQ5x68T3x55A6x33R-E5x76G-F34x51Y-E5x64A3x53GPCRInteractions:AromaticHydrophobicIonicPolarVan der waalsG Protein segments:G.S1G.H3G.h3s5G.hgh4G.H4G.S6G.H5GPCR segments:ICL2TM3ICL3TM5TM6C-term

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

ACDEFGHIKLMNPQRSTVWY-_+van-der-waalshydrophobicionicaromaticpolar
null2x393x503x533x543x5534x5034x5134x545x615x645x655x685x715x725x766x296x326x336x366x377x558x47G.H5.26G.H5.25G.H5.24G.H5.23G.H5.22G.H5.20G.H5.19G.H5.17G.H5.16G.H5.15G.H5.13G.H5.12G.H5.11G.H5.08G.S6.01G.h4s6.20G.h4s6.03G.H4.16G.H4.13G.H4.12G.H4.08G.hgh4.13G.hgh4.11G.hgh4.08G.h3s5.01G.H3.17G.S3.03G.S3.01G.S1.02G.hns1.02Res. Pos.β2 (8GEB_REFINED)- (8GEB_REFINED)-----------------------TRAITPFQVEAQKIEKKATLRSTPFQRAITVEAQKIEKKATLRSYYRIQFCHVFRHLLDRYCTLEQWYRDLRYPD

, , , GPCR
Interaction cut-off: strict (stick), loose (line)

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