VPAC1 receptor - Gs

GPCR CLASS (FAMILY)

Class B1 (Secretin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8E3Z

RESOLUTION

2.7 Å

REFERENCE

10.1038/S41467-022-34629-3

PUBLICATION DATE

Nov. 23, 2022

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

80 interactions (Click to see)

LIGANDS

Vasoactive intestinal polypeptide - Agonist pubchem

PHYSIOLOGICAL LIGANDS

PHM, GHRH, VIP, PACAP-27, PHI, secretin, PACAP-38



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM3 L 243 3x57 G.H5 Y 391 G.H5.23
Polar TM5 K 322 5x64 G.H5 Q 384 G.H5.16
Hydrophobic TM5 K 322 5x64 G.H5 Q 384 G.H5.16
Van-der-waals TM5 K 322 5x64 G.H5 Q 384 G.H5.16
Hydrophobic ICL2 F 248 - G.H5 I 383 G.H5.15
Van-der-waals ICL2 F 248 - G.H5 I 383 G.H5.15
Hydrophobic ICL3 I 328 - G.H4 R 342 G.H4.12
Van-der-waals ICL3 I 328 - G.H4 R 342 G.H4.12
Polar TM2 R 174 2x46 G.H5 Q 390 G.H5.22
Hydrophobic TM2 R 174 2x46 G.H5 Q 390 G.H5.22
Van-der-waals TM2 R 174 2x46 G.H5 Q 390 G.H5.22
Hydrophobic H8 N 392 8x47 G.H5 E 392 G.H5.24
Hydrophobic TM3 L 243 3x57 G.H5 H 387 G.H5.19
Van-der-waals TM3 L 243 3x57 G.H5 H 387 G.H5.19
Hydrophobic TM3 L 244 3x58 G.H5 L 388 G.H5.20
Van-der-waals TM3 L 244 3x58 G.H5 L 388 G.H5.20
Polar ICL3 R 329 - G.hgh4 D 323 G.hgh4.13
Hydrophobic ICL3 R 329 - G.hgh4 D 323 G.hgh4.13
Van-der-waals ICL3 R 329 - G.hgh4 D 323 G.hgh4.13
Hydrophobic TM3 Y 239 3x53 G.H5 Y 391 G.H5.23
Van-der-waals TM3 Y 239 3x53 G.H5 Y 391 G.H5.23
Polar ICL2 S 247 - G.H5 Q 384 G.H5.16
Hydrophobic ICL2 S 247 - G.H5 Q 384 G.H5.16
Van-der-waals ICL2 S 247 - G.H5 Q 384 G.H5.16
Polar TM3 A 245 3x59 G.H5 R 380 G.H5.12
Van-der-waals TM3 A 245 3x59 G.H5 R 380 G.H5.12
Hydrophobic TM4 R 252 4x40 G.HN Q 35 G.HN.52
Van-der-waals TM4 R 252 4x40 G.HN Q 35 G.HN.52
Hydrophobic ICL3 D 327 - G.H4 L 346 G.H4.16
Van-der-waals ICL3 D 327 - G.H4 L 346 G.H4.16
Hydrophobic TM6 L 345 6x44 G.H5 L 393 G.H5.25
Van-der-waals TM6 L 345 6x44 G.H5 L 393 G.H5.25
Hydrophobic TM5 L 323 5x65 G.H5 L 394 G.H5.26
Aromatic ICL2 F 248 - G.S1 H 41 G.S1.02
Hydrophobic ICL2 F 248 - G.S1 H 41 G.S1.02
Polar TM6 S 342 6x41 G.H5 L 393 G.H5.25
Hydrophobic TM5 L 319 5x61 G.H5 L 388 G.H5.20
Hydrophobic ICL2 F 248 - G.H5 C 379 G.H5.11
Hydrophobic ICL3 D 327 - G.S6 P 361 G.S6.03
Van-der-waals ICL3 D 327 - G.S6 P 361 G.S6.03
Hydrophobic ICL2 S 247 - G.H5 I 383 G.H5.15
Van-der-waals ICL2 S 247 - G.H5 I 383 G.H5.15
Ionic ICL3 D 327 - G.H4 R 342 G.H4.12
Hydrophobic TM5 L 319 5x61 G.H5 L 394 G.H5.26
Hydrophobic TM6 L 346 6x45 G.H5 L 393 G.H5.25
Hydrophobic ICL2 F 248 - G.S3 F 219 G.S3.03
Polar TM3 L 244 3x58 G.H5 Q 384 G.H5.16
Hydrophobic TM3 L 244 3x58 G.H5 Q 384 G.H5.16
Van-der-waals TM3 L 244 3x58 G.H5 Q 384 G.H5.16
Aromatic TM2 R 174 2x46 G.H5 Y 391 G.H5.23
Hydrophobic TM2 R 174 2x46 G.H5 Y 391 G.H5.23
Van-der-waals TM2 R 174 2x46 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 F 248 - G.S3 V 217 G.S3.01
Van-der-waals ICL2 F 248 - G.S3 V 217 G.S3.01
Hydrophobic ICL3 I 328 - G.H4 L 346 G.H4.16
Hydrophobic TM3 L 240 3x54 G.H5 Y 391 G.H5.23
Van-der-waals TM3 L 240 3x54 G.H5 Y 391 G.H5.23
Hydrophobic TM3 L 240 3x54 G.H5 L 393 G.H5.25
Aromatic ICL2 F 248 - G.H5 F 376 G.H5.08
Hydrophobic ICL2 F 248 - G.H5 F 376 G.H5.08
Van-der-waals ICL2 F 248 - G.H5 F 376 G.H5.08
Ionic TM5 K 322 5x64 G.H5 D 381 G.H5.13
Hydrophobic TM5 K 322 5x64 G.H5 D 381 G.H5.13
Van-der-waals TM5 K 322 5x64 G.H5 D 381 G.H5.13
Hydrophobic ICL3 P 326 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 P 326 - G.H5 R 385 G.H5.17
Polar H8 G 393 8x48 G.H5 E 392 G.H5.24
Van-der-waals H8 G 393 8x48 G.H5 E 392 G.H5.24
Polar TM5 K 322 5x64 G.H5 R 385 G.H5.17
Hydrophobic TM5 K 322 5x64 G.H5 R 385 G.H5.17
Aromatic ICL2 F 248 - G.H5 R 380 G.H5.12
Hydrophobic ICL2 F 248 - G.H5 R 380 G.H5.12
Hydrophobic TM5 K 322 5x64 G.H5 L 388 G.H5.20
Van-der-waals TM5 K 322 5x64 G.H5 L 388 G.H5.20
Polar ICL2 S 247 - G.H5 R 380 G.H5.12
Hydrophobic ICL2 S 247 - G.H5 R 380 G.H5.12
Van-der-waals ICL2 S 247 - G.H5 R 380 G.H5.12